AB235639

Mouse SAP ELISA Kit

Recombinant
SimpleStep
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Mouse SAP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse SAP with a sensitivity of 11.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Ptx2, Sap, Apcs, Serum amyloid P-component, SAP

6 Images

Sandwich ELISA - Mouse SAP ELISA Kit (AB235639)

sELISA

Supplier Data

Sandwich ELISA - Mouse SAP ELISA Kit (AB235639)

Leading Competitor Comparison.

Standard curve comparison between Mouse SAP SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Mouse SAP ELISA Kit (AB235639)

Interpolated concentrations of native SAP in mouse serum and plasma samples.

The concentrations of SAP were measured in duplicates, interpolated from the SAP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 100,000, plasma (citrate) 1 : 100,000, plasma (EDTA) 1 : 100,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SAP concentration was determined to be 197 μg/mL in neat serum, 98.7 μg/mL in neat plasma (citrate) and 176 μg/mL in neat plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Mouse SAP ELISA Kit (AB235639)

Serum samples from three strains of mice were measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SAP concentration was determined to be 193 μg/mL in neat CD-1 serum, 433 μg/mL in neat C57BL-6 serum, and 583 μg/mL in neat BALB/c serum.

sELISA

Supplier Data

Sandwich ELISA - Mouse SAP ELISA Kit (AB235639)

Representative image of Mouse SAP ELISA Kit (ab235639)

Components shown from left to right :

- Sample diluent NS

- Denaturant

- 10X Mouse SAP Capture Antibody

- 10X Mouse SAP Detector Antibody

- Mouse SAP Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Mouse SAP ELISA Kit (AB235639)

Example of mouse SAP standard curve in Sample Diluent NS + Enhancer.

The SAP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse SAP ELISA Kit (AB235639)

Interpolated concentrations of native SAP in mouse kidney and lung tissue supernatant samples.

The concentrations of SAP were measured in duplicates, interpolated from the SAP standard curves and corrected for sample dilution. Undiluted samples are as follows : mouse kidney tissue supernatant 1 : 100 and mouse lung tissue supernatant 1 : 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SAP concentration was determined to be 43 ng/mL in neat kidney tissue supernatant and 113 ng/mL in neat lung tissue supernatant.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 11.3 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse SAP ELISA Kit (ab235639) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SAP protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse SAP with 11.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Mouse Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Intra", "sample": "Mouse Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]

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Recovery

[ { "sample": "Serum", "range": "109 - 124 %", "average": "= 118" }, { "sample": "EDTA Plasma", "range": "95 - 122 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "83 - 109 %", "average": "= 97" }, { "sample": "Cell culture media", "range": "95 - 102 %", "average": "= 98" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab235639-1x96Tests", "assetComponentDetails": [ { "size":"1 x 500 µL", "name":"Denaturant", "number":"AB235639-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB235639-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB235639-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB235639-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB235639-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB235639-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB235639-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB235639-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse SAP Detector Antibody", "number":"AB235639-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB235639-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse SAP Lyophilized Recombinant Protein", "number":"AB235639-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse SAP Capture Antibody", "number":"AB235639-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Serum Amyloid P component also known as SAP P-SAP or APCS protein is a pentraxin family member. It has a molecular mass of approximately 25 kDa. SAP expresses mainly in the liver and circulates in blood plasma. The protein consists of five identical subunits which form a homopentameric structure. This structure enables SAP to bind to amyloid fibrils a defining mechanical action of this protein.

Biological function summary

SAP plays a role in the immune system by recognizing damaged cells and pathogens. It acts as an opsonin marking these cellular targets for phagocytosis. As part of a complex SAP stabilizes extracellular matrix and amyloid deposits. It binds specifically to amyloid fibrils found in various tissues potentially facilitating their removal by immune cells. The protein's binding actions are important for its role in maintaining tissue and fluid homeostasis.

Pathways

SAP is actively involved in the innate immune response and amyloidogenic pathways. It interacts with the complement system to enhance the clearance of cell debris and amyloid fibrils. The complement system is an important component of innate immunity where SAP can engage with complement proteins like C1q. This interaction suggests that SAP plays roles in modulating both inflammation and tissue repair processes.

SAP has significant connections. SAP is related to systemic amyloidosis where it binds to amyloid deposits affecting multiple organs. Moreover SAP has been implicated in Alzheimer's disease due to its interaction with beta-amyloid protein plaques. In these disorders the involvement of SAP aids researchers in understanding the pathological mechanisms and could open avenues for targeted therapies aimed at modulating SAP functions.

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Product protocols

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Target data

See full target information Apcs

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse SAP ELISA Kit

Sandwich ELISA - Mouse SAP ELISA Kit (AB235639)

Sandwich ELISA - Mouse SAP ELISA Kit (AB235639)

Sandwich ELISA - Mouse SAP ELISA Kit (AB235639)

Sandwich ELISA - Mouse SAP ELISA Kit (AB235639)

Sandwich ELISA - Mouse SAP ELISA Kit (AB235639)

Sandwich ELISA - Mouse SAP ELISA Kit (AB235639)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise