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AB100740

Mouse SCF ELISA Kit

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(5 Publications)

Mouse SCF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse SCF in Mouse, Rat in Biofluids, Cell Culture Media samples.

View Alternative Names

Kitl, Mgf, Sl, Slf, Kitlg, Kit ligand, Hematopoietic growth factor KL, Mast cell growth factor, Steel factor, Stem cell factor, c-Kit ligand, MGF, SCF

3 Images
Sandwich ELISA - Mouse SCF ELISA Kit (AB100740)
  • sELISA

Lab

Sandwich ELISA - Mouse SCF ELISA Kit (AB100740)

SCF measured in various fluids showing quantity (pg) per mL of tested sample

Sandwich ELISA - Mouse SCF ELISA Kit (AB100740)
  • sELISA

Unknown

Sandwich ELISA - Mouse SCF ELISA Kit (AB100740)

Representative standard curve using ab100740

Sandwich ELISA - Mouse SCF ELISA Kit (AB100740)
  • sELISA

Unknown

Sandwich ELISA - Mouse SCF ELISA Kit (AB100740)

Representative standard curve using ab100740

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse, Rat

Assay type

Sandwich (quantitative)

Sensitivity

< 5 pg/mL

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's SCF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse SCF in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse SCF coated on a 96-well plate. Standards and samples are pipetted into the wells and SCF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse SCF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SCF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 106 %", "average": "= 98.64" }, { "sample": "Serum", "range": "85 - 105 %", "average": "= 95.86" }, { "sample": "Plasma", "range": "84 - 104 %", "average": "= 94.56" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100740-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100740-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100740-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse SCF", "number":"AB100740-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"90X HRP-Streptavidin Concentrate", "number":"AB100740-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse SCF Standard (lyophilized)", "number":"AB100740-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SCF Microplate (12 x 8 wells)", "number":"AB100740-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100740-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100740-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100740-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Stem Cell Factor (SCF) also known as kit ligand c-kit ligand or KITLG is a critical protein involved in cell signaling. It usually appears as a 30 kDa glycoprotein. SCF is widely expressed in various tissues including the bone marrow brain and fetal liver. Its major mechanical function is to bind to the c-KIT receptor triggering various intracellular pathways that influence cell proliferation survival and differentiation. Aside from its other names SCF in mouse plasma is often monitored including in assays like c-kit ligand ELISA.
Biological function summary

SCF acts as an indispensable growth factor promoting hematopoiesis and melanogenesis. It plays a significant role in the regulation of hematopoietic stem cells and mast cells. SCF and its receptor c-KIT form a complex that activates signaling pathways essential for these processes. This protein is also involved in gametogenesis by aiding the proliferation and survival of primordial germ cells. SCF's action is not limited to mammals as studies demonstrate its activity in other species like mouse SCF.

Pathways

SCF impacts multiple important biological systems by engaging with c-KIT. The SCF/c-KIT interaction contributes to the MAPK/ERK pathway influencing cell division and growth. It also plays a role in the PI3K/AKT pathway that governs cell survival. Beyond its direct association with c-KIT SCF's involvement in these pathways connects it to several other proteins like RAS and PI3K which are important for signal transduction.

SCF has associations with several pathological conditions. Dysregulation of the SCF/c-KIT pathway can lead to disorders like mastocytosis and certain leukemias. The overactivation or mutations in c-KIT are often implicated in these diseases due to its cooperative action with SCF. Additionally aberrant SCF signaling can contribute to the progression of gastrointestinal stromal tumors (GIST) where c-KIT has known relevance. Understanding SCF's role can therefore provide insights into diagnostic and therapeutic strategies for these conditions.
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Product protocols

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Target data

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
See full target information Kitlg
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:648 PubMed35115536

2022

Endothelial cell-specific expression of serine/threonine kinase 11 modulates dendritic cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Zhao,Young-Min Han,Ping Song,Zhixue Liu,Zuyi Yuan,Ming-Hui Zou

Molecular medicine reports 24: PubMed34368880

2021

Atractylenolide‑1 alleviates gastroparesis in diabetic rats by activating the stem cell factor/c‑kit signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Li,Wen Cao,Xun-Bin Zhang,Xi-Xia Zhang,Chao Gu,Li-Mei Gu,Cheng-Yu Pan,Yao-Zhou Tian,Min Lu

International journal of molecular sciences 21: PubMed33339427

2020

Cell Sheet Comprised of Mesenchymal Stromal Cells Overexpressing Stem Cell Factor Promotes Epicardium Activation and Heart Function Improvement in a Rat Model of Myocardium Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Konstantin V Dergilev,Evgeny K Shevchenko,Zoya I Tsokolaeva,Irina B Beloglazova,Ekaterina S Zubkova,Maria A Boldyreva,Mikhail Yu Menshikov,Elizaveta I Ratner,Dmitry Penkov,Yelena V Parfyonova

Journal of molecular and cellular cardiology 53:469-74 PubMed22850284

2012

Stem cell factor is responsible for the rapid response in mature mast cell density in the acutely stressed heart.

Applications

Unspecified application

Species

Unspecified reactive species

Jianping Li,Hong Lu,Eric Plante,Giselle C Meléndez,Scott P Levick,Joseph S Janicki

American journal of physiology. Heart and circulatory physiology 302:H811-7 PubMed22160000

2011

Prevention of adverse cardiac remodeling to volume overload in female rats is the result of an estrogen-altered mast cell phenotype.

Applications

Unspecified application

Species

Rat

Hong Lu,Giselle C Meléndez,Scott P Levick,Joseph S Janicki
View all publications
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