Mouse SCF ELISA Kit

Product details

Mouse SCF ELISA Kit (ab197750) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SCF protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse SCF with 1.11 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Stem Cell Factor (SCF), also known as c-kit ligand, mast cell growth factor (MGF), and Steel Factor, is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. Mutations in either SCF or its receptor, c-kit tyrosine kinase receptor, have the same complex phenotype that affects hematopoiesis, gametogenesis, and melanogenesis. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both Myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Additionally, fibroblasts, bone marrow stromal cells, macrophages, astroglia, and neurons widely express mouse SCF.Mouse SCF cDNA encodes a 248 amino acid (aa) residue. This residue is a type I transmembrane protein containing a 25 aa signal peptide, a 189 aa extracellular region, a 23 aa TM domain, and a 36 aa cytoplasmic tail. Through alternative splicing, two isoforms of cell-associated SCF exist: KL-1 and KL-2. These isoforms differ by the presence or absence of exon 6, which encodes a proteolytic cleavage site. The 164-165 aa secreted soluble form of SCF is generated by proteolytic cleavage from KL-1. Human SCF and rat SCF are 80% and 95% identical to mouse SCF, respectively.The standard is affinity purified.