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AB100741

Mouse SDF1 ELISA Kit

4

(2 Reviews)

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(17 Publications)

Mouse SDF1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse SDF1 in Mouse in Biofluids, Cell Culture Media samples.
4 Images
ELISA - Mouse SDF1 ELISA Kit (AB100741)
  • ELISA

Lab

ELISA - Mouse SDF1 ELISA Kit (AB100741)

SDF1 measured in culture supernatants from control or activated cells (P/L), stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) with the addition of 1 ug x mL-1 of LPS (Sigma) for the last 6 hours, and in biological fluids (duplicates +/- SD). All samples tested undiluted.

ELISA - Mouse SDF1 ELISA Kit (AB100741)
  • ELISA

Lab

ELISA - Mouse SDF1 ELISA Kit (AB100741)

Standard curve with background signal subtracted (duplicates; +/- SD).

Sandwich ELISA - Mouse SDF1 ELISA Kit (AB100741)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse SDF1 ELISA Kit (AB100741)

Representative of a standard curve.

Representative Standard Curve using ab100741

Sandwich ELISA - Mouse SDF1 ELISA Kit (AB100741)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse SDF1 ELISA Kit (AB100741)

Representation of a standard curve.

Representative Standard Curve using ab100741

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 3 pg/mL

Range

9.38 - 600 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse SDF1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100741) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse SDF1 in serum, plasma and cell culture supernatants. (Mouse SDF1 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

This assay employs an antibody specific for mouse SDF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse SDF1antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 107 %", "average": "= 99.34" }, { "sample": "Serum", "range": "91 - 107 %", "average": "= 99" }, { "sample": "Plasma", "range": "90 - 107 %", "average": "= 100.36" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100741-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100741-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100741-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SDF1 Microplate (12 strips x 8 wells)", "number":"AB100741-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse SDF1", "number":"AB100741-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100741-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100741-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse SDF1 Standard (lyophilized)", "number":"AB100741-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100741-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100741-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SDF1 alpha also known as CXCL12 is a chemokine with a molecular mass of approximately 8 kDa. It plays a significant role in the immune system by directing the movement of cells through chemotaxis. This protein is predominantly expressed in various tissues including bone marrow lymph nodes and lungs. Its expression occurs across different cell types such as endothelial cells fibroblasts and stromal cells making it an essential component of the cell signaling environment.
Biological function summary

SDF1 alpha participates in regulating the movement and positioning of hematopoietic stem cells supporting tissue homeostasis and repair. It operates often by binding to the G-protein coupled receptor CXCR4. This interaction is especially important in hematopoiesis vasculogenesis and neuron guidance. Although SDF1 alpha does not function as part of a larger complex its interaction with CXCR4 plays an important role for processes like development and wound healing.

Pathways

SDF1 alpha is heavily involved in the chemokine signaling pathway which is pivotal for immune responses and cellular communication. Its interaction with CXCR4 also links it to the MAPK/ERK pathway contributing to cell proliferation differentiation and survival. These pathways highlight the essential interplay between SDF1 alpha and proteins such as CXCR4 and CCR7 which are part of broader cellular responses.

SDF1 alpha has notable implications in cancer and HIV. Its interaction with CXCR4 is important in tumor growth and metastasis influencing the tumor microenvironment. For HIV SDF1 alpha and its receptor CXCR4 are critical in viral entry into host cells. A deeper understanding of these relationships can aid in identifying therapeutic targets and diagnosing these conditions effectively.
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Product protocols

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Target data

See full target information Cxcl12
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

The journal of headache and pain 25:213 PubMed39627724

2024

Elevated plasma CXCL12 leads to pain chronicity via positive feedback upregulation of CXCL12/CXCR4 axis in pain synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Ze Leng,Mei-Jia Fang,Yi-Min Wang,Zhen-Jia Lin,Qian-Yi Li,Ya-Nan Xu,Chun-Lin Mai,Jun-Ya Wan,Yangyinhui Yu,Ming Wei,Ying Li,Yu-Fan Zheng,Kai-Lang Zhang,Ya-Juan Wang,Li-Jun Zhou,Zhi Tan,Hui Zhang

Biomolecules 14: PubMed39456139

2024

Impact of SDF-1 and AMD3100 on Hair Follicle Dynamics in a Chronic Stress Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yinglin Zhao,Wenzi Liang,Zhehui Liu,Xiuwen Chen,Changmin Lin

Frontiers in endocrinology 14:1252727 PubMed37810891

2023

Potential involvement of the bone marrow in experimental Graves' disease and thyroid eye disease.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Gulbins,Mareike Horstmann,Simone Keitsch,Matthias Soddemann,Barbara Wilker,Gregory C Wilson,Ryan Zeidan,Gary D Hammer,Anke Daser,Nikolaos E Bechrakis,Gina-Eva Görtz,Anja Eckstein

Nature communications 14:5500 PubMed37679321

2023

Astrocyte reactivity and inflammation-induced depression-like behaviors are regulated by Orai1 calcium channels.

Applications

Unspecified application

Species

Unspecified reactive species

Michaela M Novakovic,Kirill S Korshunov,Rogan A Grant,Megan E Martin,Hiam A Valencia,G R Scott Budinger,Jelena Radulovic,Murali Prakriya

Acta pharmaceutica Sinica. B 13:2202-2218 PubMed37250158

2023

Click chemistry extracellular vesicle/peptide/chemokine nanocarriers for treating central nervous system injuries.

Applications

Unspecified application

Species

Unspecified reactive species

Huitong Ruan,Yongfang Li,Cheng Wang,Yixu Jiang,Yulong Han,Yiwei Li,Dandan Zheng,Jing Ye,Gang Chen,Guo-Yuan Yang,Lianfu Deng,Ming Guo,Xingcai Zhang,Yaohui Tang,Wenguo Cui

PLoS biology 21:e3001746 PubMed37134077

2023

Tumor-derived interleukin-1α and leukemia inhibitory factor promote extramedullary hematopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Derek A G Barisas,Ashraf Ul Kabir,Jun Wu,Karen Krchma,Minseo Kim,Madhav Subramanian,Bernd H Zinselmeyer,Colin L Stewart,Kyunghee Choi

Nature immunology 23:605-618 PubMed35352063

2022

B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian J Schloss,Maarten Hulsmans,David Rohde,I-Hsiu Lee,Nicolas Severe,Brody H Foy,Fadi E Pulous,Shuang Zhang,Konstantinos D Kokkaliaris,Vanessa Frodermann,Gabriel Courties,Chongbo Yang,Yoshiko Iwamoto,Anders Steen Knudsen,Cameron S McAlpine,Masahiro Yamazoe,Stephen P Schmidt,Gregory R Wojtkiewicz,Gustavo Santos Masson,Karin Gustafsson,Diane Capen,Dennis Brown,John M Higgins,David T Scadden,Peter Libby,Filip K Swirski,Kamila Naxerova,Matthias Nahrendorf

Theranostics 11:1059-1078 PubMed33391521

2021

CXCL12-mediated monocyte transmigration into brain perivascular space leads to neuroinflammation and memory deficit in neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Lin Mai,Zhi Tan,Ya-Nan Xu,Jing-Jun Zhang,Zhen-Hua Huang,Dong Wang,Hui Zhang,Wen-Shan Gui,Jun Zhang,Zhen-Jia Lin,Ying-Tong Meng,Xiao Wei,Ying-Tao Jie,Peter M Grace,Long-Jun Wu,Li-Jun Zhou,Xian-Guo Liu

Drug and alcohol dependence 212:108004 PubMed32408137

2020

Voluntary wheel running protects against the increase in ethanol consumption induced by social stress in mice.

Applications

Unspecified application

Species

Unspecified reactive species

M D Reguilón,C Ferrer-Pérez,R Ballestín,J Miñarro,M Rodríguez-Arias

Nature medicine 25:1761-1771 PubMed31700184

2019

Exercise reduces inflammatory cell production and cardiovascular inflammation via instruction of hematopoietic progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Frodermann,David Rohde,Gabriel Courties,Nicolas Severe,Maximilian J Schloss,Hajera Amatullah,Cameron S McAlpine,Sebastian Cremer,Friedrich F Hoyer,Fei Ji,Ian D van Koeverden,Fanny Herisson,Lisa Honold,Gustavo Santos Masson,Shuang Zhang,Jana Grune,Yoshiko Iwamoto,Stephen P Schmidt,Gregory R Wojtkiewicz,I-Hsiu Lee,Karin Gustafsson,Gerard Pasterkamp,Saskia C A de Jager,Ruslan I Sadreyev,Jean MacFadyen,Peter Libby,Paul Ridker,David T Scadden,Kamila Naxerova,Kate L Jeffrey,Filip K Swirski,Matthias Nahrendorf
View all publications
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