Mouse SDF1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse SDF1 in Mouse in Plasma, Cell culture supernatant, Serum samples.
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- Frontiers in endocrinology 14:12527272023Potential involvement of the bone marrow in experimental Graves' disease and thyroid eye disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne Gulbins,Mareike Horstmann,Simone Keitsch,Matthias Soddemann,Barbara Wilker,Gregory C Wilson,Ryan Zeidan,Gary D Hammer,Anke Daser,Nikolaos E Bechrakis,Gina-Eva Görtz,Anja EcksteinPubMed 37810891
- Nature communications 14:55002023Astrocyte reactivity and inflammation-induced depression-like behaviors are regulated by Orai1 calcium channels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichaela M Novakovic,Kirill S Korshunov,Rogan A Grant,Megan E Martin,Hiam A Valencia,G R Scott Budinger,Jelena Radulovic,Murali PrakriyaPubMed 37679321
- Acta pharmaceutica Sinica. B 13:2202-22182023Click chemistry extracellular vesicle/peptide/chemokine nanocarriers for treating central nervous system injuries.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuitong Ruan,Yongfang Li,Cheng Wang,Yixu Jiang,Yulong Han,Yiwei Li,Dandan Zheng,Jing Ye,Gang Chen,Guo-Yuan Yang,Lianfu Deng,Ming Guo,Xingcai Zhang,Yaohui Tang,Wenguo CuiPubMed 37250158
- PLoS biology 21:e30017462023Tumor-derived interleukin-1α and leukemia inhibitory factor promote extramedullary hematopoiesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDerek A G Barisas,Ashraf Ul Kabir,Jun Wu,Karen Krchma,Minseo Kim,Madhav Subramanian,Bernd H Zinselmeyer,Colin L Stewart,Kyunghee ChoiPubMed 37134077
- Nature immunology 23:605-6182022B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaximilian J Schloss,Maarten Hulsmans,David Rohde,I-Hsiu Lee,Nicolas Severe,Brody H Foy,Fadi E Pulous,Shuang Zhang,Konstantinos D Kokkaliaris,Vanessa Frodermann,Gabriel Courties,Chongbo Yang,Yoshiko Iwamoto,Anders Steen Knudsen,Cameron S McAlpine,Masahiro Yamazoe,Stephen P Schmidt,Gregory R Wojtkiewicz,Gustavo Santos Masson,Karin Gustafsson,Diane Capen,Dennis Brown,John M Higgins,David T Scadden,Peter Libby,Filip K Swirski,Kamila Naxerova,Matthias NahrendorfPubMed 35352063
- Theranostics 11:1059-10782021CXCL12-mediated monocyte transmigration into brain perivascular space leads to neuroinflammation and memory deficit in neuropathic pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Lin Mai et. al.PubMed 33391521
- Drug and alcohol dependence 212:1080042020Voluntary wheel running protects against the increase in ethanol consumption induced by social stress in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM D Reguilón et. al.PubMed 32408137
- Nature medicine 25:1761-17712019Exercise reduces inflammatory cell production and cardiovascular inflammation via instruction of hematopoietic progenitor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVanessa Frodermann et. al.PubMed 31700184
- Artificial cells, nanomedicine, and biotechnology 47:980-9882019Accelerated infected wound healing by topical application of encapsulated Rosemary essential oil into nanostructured lipid carriers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeyvan Khezri,Mohammad Reza Farahpour,Shokoofeh Mounesi RadPubMed 30857435
- Cell death & disease 10:1912019CXCR7 promotes melanoma tumorigenesis via Src kinase signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiran Xu et. al.PubMed 30804329
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 9.38 - 600 pg/mL
- Sensitivity
- < 3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Mouse SDF1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse SDF1 in Mouse in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 9.38 - 600 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 3 pg/mL
- General recovery
- 99%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 99.34 | Range 89 - 107 % |
Sample type Serum | Average % = 99 | Range 91 - 107 % |
Sample type Plasma | Average % = 100.36 | Range 90 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Mouse SDF1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100741) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse SDF1 in serum, plasma and cell culture supernatants. (Mouse SDF1 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).
This assay employs an antibody specific for mouse SDF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse SDF1antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SDF1 alpha also known as CXCL12 is a chemokine with a molecular mass of approximately 8 kDa. It plays a significant role in the immune system by directing the movement of cells through chemotaxis. This protein is predominantly expressed in various tissues including bone marrow lymph nodes and lungs. Its expression occurs across different cell types such as endothelial cells fibroblasts and stromal cells making it an essential component of the cell signaling environment.
- Biological function summary
SDF1 alpha participates in regulating the movement and positioning of hematopoietic stem cells supporting tissue homeostasis and repair. It operates often by binding to the G-protein coupled receptor CXCR4. This interaction is especially important in hematopoiesis vasculogenesis and neuron guidance. Although SDF1 alpha does not function as part of a larger complex its interaction with CXCR4 plays an important role for processes like development and wound healing.
- Pathways
SDF1 alpha is heavily involved in the chemokine signaling pathway which is pivotal for immune responses and cellular communication. Its interaction with CXCR4 also links it to the MAPK/ERK pathway contributing to cell proliferation differentiation and survival. These pathways highlight the essential interplay between SDF1 alpha and proteins such as CXCR4 and CCR7 which are part of broader cellular responses.
- Associated diseases and disorders
SDF1 alpha has notable implications in cancer and HIV. Its interaction with CXCR4 is important in tumor growth and metastasis influencing the tumor microenvironment. For HIV SDF1 alpha and its receptor CXCR4 are critical in viral entry into host cells. A deeper understanding of these relationships can aid in identifying therapeutic targets and diagnosing these conditions effectively.
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4 product images
ELISA - Mouse SDF1 ELISA Kit (ab100741)
SDF1 measured in culture supernatants from control or activated cells (P/L), stimulated for 24 hours with 50 ng x mL-1 of PMA (Phorbol 12-myristate 13-acetate (PMA), PKC activator ab120297) with the addition of 1 ug x mL-1 of LPS (Sigma) for the last 6 hours, and in biological fluids (duplicates +/- SD). All samples tested undiluted.
ELISA - Mouse SDF1 ELISA Kit (ab100741)
Standard curve with background signal subtracted (duplicates; +/- SD).
Sandwich ELISA - Mouse SDF1 ELISA Kit (ab100741)
Representation of a standard curve.
Representative Standard Curve using ab100741
Sandwich ELISA - Mouse SDF1 ELISA Kit (ab100741)
Representative of a standard curve.
Representative Standard Curve using ab100741
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