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AB325454

Mouse Serum Albumin ELISA Kit, Chemiluminescent

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Mouse Serum Albumin ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Mouse Serum Albumin with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :4.6 - 10000 pg/mL with a sensitivity of 1.4 pg/mL
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Alb-1, Alb1, Alb, Albumin

5 Images
Sandwich ELISA - Mouse Serum Albumin ELISA Kit, Chemiluminescent (AB325454)
  • sELISA

Lab

Sandwich ELISA - Mouse Serum Albumin ELISA Kit, Chemiluminescent (AB325454)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Mouse Serum Albumin ELISA Kit, Chemiluminescent (AB325454)
  • sELISA

Lab

Sandwich ELISA - Mouse Serum Albumin ELISA Kit, Chemiluminescent (AB325454)

Interpolated concentration of native Albumin was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 10,000,000 mouse serum, plasma-citrate, plasma-EDTA and plasma-heparin. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse Serum Albumin ELISA Kit, Chemiluminescent (AB325454)
  • sELISA

Lab

Sandwich ELISA - Mouse Serum Albumin ELISA Kit, Chemiluminescent (AB325454)

Dynamic range comparison between the Chemiluminescent Mouse Albumin SimpleStep ELISA and colorimetric Mouse Albumin SimpleStep ELISA (ab207620). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Mouse Serum Albumin ELISA Kit, Chemiluminescent (AB325454)
  • sELISA

Lab

Sandwich ELISA - Mouse Serum Albumin ELISA Kit, Chemiluminescent (AB325454)

Standard curve comparison between the Chemiluminescent Mouse Albumin SimpleStep ELISA and colorimetric Mouse Albumin SimpleStep ELISA (ab207620) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Serum Albumin ELISA Kit, Chemiluminescent (AB325454)
  • sELISA

Lab

Sandwich ELISA - Mouse Serum Albumin ELISA Kit, Chemiluminescent (AB325454)

Interpolated concentration of native Albumin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Urine 1 : 1,600. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Urine

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 1.4 pg/mL

Range

4.6 - 10000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Serum Albumin ELISA Kit, Chemiluminescent (ab325454) is a rapid single-wash sandwich 55-min ELISA to measure Mouse Serum Albumin in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Urine  with a sensitivity of 1.4 pg/ml

How the assay works

Mouse Serum Albumin ELISA Kit, Chemiluminescent (ab325454) SimpleStep ELISA®  employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.<br>Please refer to our protocol booklet for more details.

Mouse Serum Albumin (ab325454) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-mouse Serum Albumin antibody [EPR18741-18] - BSA and Azide free (Capture) ab244635

- Anti-mouse Serum Albumin antibody [EPR18741-67] - BSA and Azide free (Detector) ab242698

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.3" } ]
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Recovery

[ { "sample": "Serum", "range": "96 - 99 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "84 - 99 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "84 - 101 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "104 - 108 %", "average": "= 107" }, { "sample": "Urine", "range": "73 - 107 %", "average": "= 87" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (By similarity). Does not prevent iron uptake by the bacterial siderophore aerobactin (By similarity).
See full target information Alb
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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