AB252366

Mouse SFTPC ELISA Kit

Recombinant
SimpleStep
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Mouse SFTPC ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse SFTPC with a sensitivity of 40 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Sftp2, Sftpc, Surfactant protein C, SP-C, Pulmonary surfactant-associated protein C, Pulmonary surfactant-associated proteolipid SPL(Val), SP5

4 Images

ELISA - Mouse SFTPC ELISA Kit (AB252366)

ELISA

Supplier Data

ELISA - Mouse SFTPC ELISA Kit (AB252366)

sELISA

Unknown

Sandwich ELISA - Mouse SFTPC ELISA Kit (AB252366)

Interpolated concentrations of native SFTPC in mouse tissue supernatant and tissue samples.

The concentrations of SFTPC were measured in duplicates, interpolated from the SFTPC standard curves and corrected for sample dilution. Undiluted samples are as follows : Mouse lung supernatant 50%, mouse lung extract 25 mg/mL. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SFTPC concentration was determined to be 5.31 ng/mL in lung supernatant and 3.99 ng/mL in lung extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse SFTPC ELISA Kit (AB252366)

Representative image of Mouse SFTPC ELISA Kit (ab252366)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse SFTPC Capture Antibody

- 10X Mouse SFTPC Detector Antibody

- Mouse SFTPC Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Sample diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Unknown

Sandwich ELISA - Mouse SFTPC ELISA Kit (AB252366)

Example of mouse SFTPC standard curve in 1X Cell Extraction Buffer PTR.

The SFTPC standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Tissue Culture Media

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 40 pg/mL

Range

117.19 - 7500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse SFTPC ELISA Kit ab252366 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse SFTPC in tissue, tissue extracts. This SimpleStep sensitivity is 40 pg/mL.

How the assay works

Mouse SFTPC SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse SFTPC ELISA Kit ab252366 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Prosurfactant Protein C antibody [EPR21012-72] - BSA and Azide free (Capture) ab259668
- Anti-Prosurfactant Protein C antibody [EPR21012-112] - BSA and Azide free (Detector) ab259668

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]

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Recovery

[ { "sample": "Tissue Culture Media", "range": "99 - 108 %", "average": "= 104" }, { "sample": "Tissue Extracts", "range": "99 - 110 %", "average": "= 105" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab252366-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB252366-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB252366-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB252366-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB252366-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB252366-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB252366-CMP12", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB252366-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB252366-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse SFTPC Detector Antibody", "number":"AB252366-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB252366-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse SFTPC Capture Antibody", "number":"AB252366-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse SFTPC Lyophilized Recombinant Protein", "number":"AB252366-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prosurfactant Protein C (Pro-SP-C) often referred to as SPC or by its abbreviated form SP-C is a hydrophobic peptide critical for lung function. This protein has a mass of approximately 21 kDa and is expressed prominently in the alveolar type II cells of the lung tissue. Beyond its full-length variant various pro forms such as proSP-C are studied for their roles during protein maturation and trafficking. These pro forms are essential for producing the mature functional SP-C a critical component of lung surfactant which reduces surface tension in alveoli and prevents lung collapse.

Biological function summary

In surfactant homeostasis Prosurfactant Protein C associates with surfactant protein complexes where it interacts with phospholipids like phosphatidylcholine to stabilize the alveolar structures. As part of this complex it aids in reducing surface tension at the air-liquid interface in the alveoli and contributes to the host defense system by fostering pathogen clearance and modulating inflammation. The intricate processing of Pro-SP-C into its mature form involves fucosylation and other post-translational modifications which are key for functional surfactant activity.

Pathways

The role of Prosurfactant Protein C is integral to the surfactant metabolism pathway affecting lung function and homeostasis. This pathway is important for proper respiratory mechanics and is closely linked to other surfactant proteins like SP-B and SP-D. These proteins work together within the lipid-protein matrix of the surfactant to ensure proper alveolar deflation and reinflation during respiration. Disruption within these pathways can have significant impact on lung health and respiratory physiology.

Mutations or dysfunctions in Prosurfactant Protein C are linked to interstitial lung diseases including pulmonary alveolar proteinosis and idiopathic pulmonary fibrosis. These conditions often result from misfolded proteins or improper surfactant metabolism leading to compromised lung function. Connections with disorders like these highlight interactions between Prosurfactant Protein C and C-reactive protein uncovering underlying inflammatory processes. Therapy and research often target these protein interactions and modifications as understanding them holds potential for strategic intervention in surfactant-related pathologies.

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Product protocols

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Target data

Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.

See full target information Sftpc

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse SFTPC ELISA Kit

ELISA - Mouse SFTPC ELISA Kit (AB252366)

Sandwich ELISA - Mouse SFTPC ELISA Kit (AB252366)

Sandwich ELISA - Mouse SFTPC ELISA Kit (AB252366)

Sandwich ELISA - Mouse SFTPC ELISA Kit (AB252366)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Assay Kits

Secondary Antibodies

Assay Kits

Product promise