AB206309

Mouse SHP-2 ELISA Kit (PTPN11)

Recombinant
SimpleStep
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Mouse SHP-2 ELISA Kit (PTPN11) is a single-wash 90-min Simplestep used to quantify Mouse SHP-2 (PTPN11) with a sensitivity of 239 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase SYP, SH-PTP2, SHP-2, Shp2, Ptpn11

2 Images

Sandwich ELISA - Mouse SHP-2 ELISA Kit (PTPN11) (AB206309)

sELISA

Supplier Data

Sandwich ELISA - Mouse SHP-2 ELISA Kit (PTPN11) (AB206309)

Example of SHP-2 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse SHP-2 ELISA Kit (PTPN11) (AB206309)

Interpolated concentrations of SHP-2 in PC12 (rat), L929 (mouse) and HeLa (human) cell extracts.

The concentrations of SHP-2 were measured in duplicate and interpolated from the SHP-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SHP-2 concentration was determined to be 79.6 pg/μg PC12 cell extract, 37.3 pg/μg L929 cell extract, and 23 pg/μg HeLa cell extract.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 239 pg/mL

Range

0.63 - 40 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse SHP-2 ELISA Kit (PTPN11) (ab206309) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SHP-2 (PTPN11) protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse SHP-2 (PTPN11) with 239 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

SHP-2 (Tyrosine-protein phosphatase non-receptor type 11 or PTPN11) is a widely expressed protein tyrosine phosphatase that functions in intracellular signal transduction. SHP-2 contains two phospho-tyrosine binding Src homology domains. SHP-2 is mutated in Noonan syndrome 1. Human, mouse and rat SHP-2 have >99% sequence identity on the amino acid level.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "PC12 Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.1" }, { "reproducibilityType": "Intra", "sample": "PC12 Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.7" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "95 - 112 %", "average": "= 103" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SHP2 also known as PTPN11 is a protein tyrosine phosphatase with a molecular mass of approximately 68 kDa. It is expressed in various tissues including the heart liver and immune cells. SHP2 belongs to the non-receptor class of protein tyrosine phosphatases and plays a critical role in cell signaling by acting as a regulator of signal transduction processes. SHP2 mediates these processes by dephosphorylating specific phosphotyrosine residues on target proteins influencing various cellular functions like proliferation differentiation and survival.

Biological function summary

The role of SHP2 extends to involvement in several signaling cascades such as the Ras/MAPK and PI3K/AKT pathways. It functions as an essential component within protein complexes that facilitate cell communication and response to external signals. The protein modulates growth factor signaling and cytokine signaling highlighting its significance in normal cell function and development. SHP2's statement in signaling processes makes it an important regulator of cellular dynamics.

Pathways

SHP2 participates in the Ras/MAPK and PI3K/AKT signaling pathways which are important for regulating cell growth survival and differentiation. Within these pathways SHP2 interacts with various signaling molecules including Grb2 Sos and Gab family adaptors. These interactions coordinate cellular responses to growth factors and other extracellular cues ensuring proper pathway activation and control. By serving as a critical mediator SHP2 integrates signals that are necessary for appropriate cellular outcomes.

SHP2 is associated with several conditions such as Noonan syndrome and various cancers. Mutations in the PTPN11 gene which encodes SHP2 often result in aberrant signaling that leads to developmental anomalies or tumorigenesis. In Noonan syndrome the mutated SHP2 protein results in disrupted Ras/MAPK pathway signaling. As for cancers SHP2 is often found to be overactive leading to enhanced cell proliferation and survival. In these contexts SHP2 is interconnected with other proteins like RAS and RAF which also contribute to oncogenic pathway activation and disease progression.

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Product protocols

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Target data

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed : 14967142). Positively regulates MAPK signal transduction pathway (By similarity). Dephosphorylates GAB1, ARHGAP35 and EGFR (By similarity). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (By similarity). Dephosphorylates CDC73 (By similarity). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (PubMed : 29644115). Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L (By similarity).

See full target information Ptpn11

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse SHP-2 ELISA Kit (PTPN11)

Sandwich ELISA - Mouse SHP-2 ELISA Kit (PTPN11) (AB206309)

Sandwich ELISA - Mouse SHP-2 ELISA Kit (PTPN11) (AB206309)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise