Mouse SHP-2 ELISA Kit (PTPN11) is a single-wash 90-min Simplestep used to quantify Mouse SHP-2 (PTPN11) with a sensitivity of 239 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.63 - 40 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 239 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed:14967142). Positively regulates MAPK signal transduction pathway (By similarity). Dephosphorylates GAB1, ARHGAP35 and EGFR (By similarity). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (By similarity). Dephosphorylates CDC73 (By similarity). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (PubMed:29644115). Dephosphorylates tyrosine-phosphorylated NEDD9/CAS-L (By similarity).
Alternative names
Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase SYP, SH-PTP2, SHP-2, Shp2, Ptpn11
What's included?
Recommended products
Mouse SHP-2 ELISA Kit (PTPN11) is a single-wash 90-min Simplestep used to quantify Mouse SHP-2 (PTPN11) with a sensitivity of 239 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.63 - 40 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 239 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample PC12 Extract | n 5 | mean - | SD - | C.V. = 2.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample PC12 Extract | n 3 | mean - | SD - | C.V. = 4.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 103 | Range 95 - 112 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse SHP-2 ELISA Kit (PTPN11) (ab206309) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SHP-2 (PTPN11) protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse SHP-2 (PTPN11) with 239 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
SHP-2 (Tyrosine-protein phosphatase non-receptor type 11 or PTPN11) is a widely expressed protein tyrosine phosphatase that functions in intracellular signal transduction. SHP-2 contains two phospho-tyrosine binding Src homology domains. SHP-2 is mutated in Noonan syndrome 1. Human, mouse and rat SHP-2 have >99% sequence identity on the amino acid level.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SHP2 also known as PTPN11 is a protein tyrosine phosphatase with a molecular mass of approximately 68 kDa. It is expressed in various tissues including the heart liver and immune cells. SHP2 belongs to the non-receptor class of protein tyrosine phosphatases and plays a critical role in cell signaling by acting as a regulator of signal transduction processes. SHP2 mediates these processes by dephosphorylating specific phosphotyrosine residues on target proteins influencing various cellular functions like proliferation differentiation and survival.
- Biological function summary
The role of SHP2 extends to involvement in several signaling cascades such as the Ras/MAPK and PI3K/AKT pathways. It functions as an essential component within protein complexes that facilitate cell communication and response to external signals. The protein modulates growth factor signaling and cytokine signaling highlighting its significance in normal cell function and development. SHP2's statement in signaling processes makes it an important regulator of cellular dynamics.
- Pathways
SHP2 participates in the Ras/MAPK and PI3K/AKT signaling pathways which are important for regulating cell growth survival and differentiation. Within these pathways SHP2 interacts with various signaling molecules including Grb2 Sos and Gab family adaptors. These interactions coordinate cellular responses to growth factors and other extracellular cues ensuring proper pathway activation and control. By serving as a critical mediator SHP2 integrates signals that are necessary for appropriate cellular outcomes.
- Associated diseases and disorders
SHP2 is associated with several conditions such as Noonan syndrome and various cancers. Mutations in the PTPN11 gene which encodes SHP2 often result in aberrant signaling that leads to developmental anomalies or tumorigenesis. In Noonan syndrome the mutated SHP2 protein results in disrupted Ras/MAPK pathway signaling. As for cancers SHP2 is often found to be overactive leading to enhanced cell proliferation and survival. In these contexts SHP2 is interconnected with other proteins like RAS and RAF which also contribute to oncogenic pathway activation and disease progression.
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2 product images
Sandwich ELISA - Mouse SHP-2 ELISA Kit (PTPN11) (ab206309)
Example of SHP-2standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse SHP-2 ELISA Kit (PTPN11) (ab206309)
Interpolated concentrations of SHP-2 in PC12 (rat), L929 (mouse) and HeLa (human) cell extracts.
The concentrations of SHP-2 were measured in duplicate and interpolated from the SHP-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SHP-2 concentration was determined to be 79.6 pg/μg PC12 cell extract, 37.3 pg/μg L929 cell extract, and 23 pg/μg HeLa cell extract.
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