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AB267583

Mouse SLPI ELISA kit (ALP)

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(4 Publications)

Mouse SLPI ELISA kit (ALP) is a Sandwich ELISA for the measurement of Mouse SLPI (ALP) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Antileukoproteinase, ALP, Secretory leukocyte protease inhibitor, Slpi

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Sandwich ELISA - Mouse SLPI ELISA kit (ALP) (AB267583)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse SLPI ELISA kit (ALP) (AB267583)

Mouse SLPI ELISA kit example standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.41 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse SLPI ELISA Kit (ALP) is designed for the quantitative determination of SLPI in cell culture supernatants, plasma and serum samples.

Δ Note: Mouse SLPI concentration is low in normal serum/plasma and may not be detectable in this assay.

This assay employs an antibody specific for mouse SLPI coated on a 96-well plate. Standards and samples are pipetted into the wells and SLPI present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse SLPI antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SLPI bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-H2, BLAME, BMP-8, CD28, Common beta Chain (CD131), Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF-17, FOLR2, Galectin-8, Glypican 1, Glypican 5, GHR, IFNgamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL-7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "103 - 146 %", "average": "= 126.4" }, { "sample": "Plasma", "range": "101 - 147 %", "average": "= 123.4" }, { "sample": "Cell culture media", "range": "94 - 148 %", "average": "= 119.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267583-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267583-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267583-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267583-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Mouse SLPI coated Microplate (12 x 8 wells)", "number":"AB267583-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB267583-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB267583-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse SLPI Standard (Lyophilized)", "number":"AB267583-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Mouse SLPI Detection Antibody", "number":"AB267583-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267583-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Secretory leukocyte protease inhibitor (SLPI) also known as antileukoproteinase and neutrophil elastase inhibitor is a protein with a molecular mass of approximately 12 kDa. SLPI is found mainly in epithelial tissues and is expressed in locations such as the respiratory tract skin and cervix. Mechanically SLPI functions as a serine protease inhibitor binding to and neutralizing enzymes like neutrophil elastase cathepsin G and trypsin which helps to limit tissue damage during inflammation.
Biological function summary

SLPI serves as a protector of epithelial surfaces from proteolytic degradation and acts as an anti-inflammatory agent. It displays broad-spectrum antimicrobial properties providing protection against bacterial and viral infections. Although SLPI is not part of a complex itself its interactions with extracellular matrix proteins help modulate wound healing processes and maintain tissue integrity.

Pathways

SLPI participates in the innate immune response and inflammation regulation pathways. It interacts within pathways that involve the complement cascade and kallikrein-kinin system helping to regulate inflammation and modulate immune responses. During these processes SLPI is related to proteins such as alpha-1-antitrypsin in its role as a protease inhibitor contributing to the regulation of protease activity and inflammatory responses.

SLPI is implicated in conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis where excessive protease activity leads to tissue damage. In these diseases SLPI shows a protective role against lung tissue degradation. SLPI is also associated with the progression of certain cancers like ovarian cancer where its overexpression may support tumor growth and metastasis. In these contexts SLPI relates to other protease inhibitors such as tissue inhibitors of metalloproteinases (TIMPs) due to their shared function in regulating protease activity and tissue remodeling.
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Product protocols

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Target data

Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G (PubMed : 9126337). Modulates the innate immune response after bacterial infection (PubMed : 12615907). Contributes to regulate the inflammatory and immune responses to the intracellular parasite L.major (PubMed : 25030421). Down-regulates responses to bacterial lipopolysaccharide (LPS) (PubMed : 12615907, PubMed : 25030421, PubMed : 9039268). Plays a role in regulating the activation of NF-kappa-B and inflammatory responses (PubMed : 11017147, PubMed : 12615907). Has antimicrobial activity against mycobacteria, but not against salmonella (PubMed : 18322212). Contributes to normal resistance against infection by M.tuberculosis (PubMed : 18322212). Required for normal resistance to L.major (PubMed : 25030421). Required for normal wound healing, probably by preventing tissue damage by limiting protease activity (PubMed : 11017147, PubMed : 25030421). Together with ELANE, required for normal differentiation and proliferation of bone marrow myeloid cells (By similarity).
See full target information Slpi
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 30:1829-1848 PubMed37337032

2023

GAS5 protects against nonalcoholic fatty liver disease via miR-28a-5p/MARCH7/NLRP3 axis-mediated pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tianxing Chen,Yao Meng,Zhihang Zhou,Haitao Li,Lingfeng Wan,Aiwen Kang,Wei Guo,Ke Ren,Xueru Song,Yu Chen,Wei Zhao

Frontiers in pharmacology 13:893336 PubMed35774609

2022

Exploring Ganweikang Tablet as a Candidate Drug for NAFLD Through Network Pharmacology Analysis and Experimental Validation.

Applications

Unspecified application

Species

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Chuanrui Ma,Xinyu Wang,Jing Zhang,Yun Zhao,Yunqing Hua,Chao Zhang,Guobin Zheng,Guangyan Yang,Jianli Guan,Huahuan Li,Meng Li,Lin Kang,Jiaqing Xiang,Guanwei Fan,Shu Yang

Bioengineered 12:2459-2468 PubMed34227919

2021

Pyrroloquinoline quinone (PQQ) alleviated sepsis-induced acute liver injury, inflammation, oxidative stress and cell apoptosis by downregulating CUL3 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhong Wu,Meiling Zhao,Zhaoheng Lin

British journal of pharmacology 178:2443-2460 PubMed33661551

2021

Tectorigenin alleviates intrahepatic cholestasis by inhibiting hepatic inflammation and bile accumulation via activation of PPARγ.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqing Xiang,Guangyan Yang,Chuanrui Ma,Lingling Wei,Han Wu,Wei Zhang,Xiuhua Tao,Lingyun Jiang,Zhen Liang,Lin Kang,Shu Yang
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