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AB277724

Mouse soluble IL-2 Receptor ELISA Kit

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Mouse soluble IL-2 Receptor ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse soluble IL-2 Receptor with a sensitivity of 15 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD25, Il2r, Il2ra, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55

2 Images
Sandwich ELISA - Mouse soluble IL-2 Receptor ELISA Kit (AB277724)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse soluble IL-2 Receptor ELISA Kit (AB277724)

Interpolated concentrations of native soluble IL-2 Receptor in mouse serum, plasma and spleen supernatant samples.

The concentrations of soluble IL-2 Receptor were measured in duplicates, interpolated from the soluble IL-2 Receptor standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 25% and spleen supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean soluble IL-2 Receptor concentration was determined to be 7,908 pg/mL in neat serum, 7,484 pg/mL in neat plasma (citrate), 5,442 pg/mL in neat plasma (EDTA), 7,172 pg/mL in neat plasma (heparin) and 837 pg/mL in neat spleen supernatant.

Sandwich ELISA - Mouse soluble IL-2 Receptor ELISA Kit (AB277724)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse soluble IL-2 Receptor ELISA Kit (AB277724)

Example of mouse soluble IL-2 Receptor standard curve in Sample Diluent NS.

The soluble IL-2 Receptor standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 15 pg/mL

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human NFAT2 ELISA kit (ab277724) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human NFAT2 protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human NFAT2 with 4 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interleukin-2 Receptor is a plasma membrane receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. Interleukin-2 receptor is a non-covalent dimer of an alpha and a beta subunit. Interleukin-2 receptor exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. This kit is designed to detect the soluble form of Interleukin-2 receptor subunit alpha (soluble IL-2 Receptor).

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "101 - 107 %", "average": "= 105" }, { "sample": "Serum", "range": "79 - 112 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "79 - 107 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "104 - 105 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "86 - 118 %", "average": "= 97" }, { "sample": "Cell culture media", "range": "119 - 122 %", "average": "= 120" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277724-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB277724-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB277724-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB277724-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB277724-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB277724-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB277724-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB277724-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse soluble IL-2 Receptor Detector Antibody", "number":"AB277724-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse soluble IL-2 Receptor Lyophilized Recombinant Protein", "number":"AB277724-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse soluble IL-2 Receptor Capture Antibody", "number":"AB277724-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
See full target information Il2ra
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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