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AB309114

Mouse SOST ELISA Kit

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Mouse SOST ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse SOST in Mouse in Cell Culture Media, Biofluids samples.

View Alternative Names

Sclerostin, Sost

3 Images
Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)

Example of mouse SOST standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)

Interpolated concentration of native SOST was measured in duplicate at different sample concentrations. Undiluted samples are 100% serum, plasma (citrate), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse SOST ELISA Kit (AB309114)

Standard curve comparison between mouse SOST SimpleStep ELISA kit (ab309114) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, EDTA Plasma, Citrate plasma, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 2.1 pg/mL

Range

10.938 - 700 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse SOST SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SOST protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Mouse SOST with 2.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 98 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "87 - 103 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "91 - 109 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "83 - 110 %", "average": "= 95" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309114-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB309114-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB309114-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB309114-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB309114-CMP06", "productcode":"" }, { "size":"1 x 6 Vial", "name":"Antibody Diluent CPI2", "number":"AB309114-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse SOST Lyophilized Recombinant Protein", "number":"AB309114-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse SOST Capture Antibody", "number":"AB309114-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB309114-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB309114-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse SOST Detector Antibody", "number":"AB309114-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
See full target information Sost
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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