AB240683

Mouse SP-D ELISA Kit

Recombinant
SimpleStep
What is this?

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(2 Publications)

Mouse SP-D ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse SP-D with a sensitivity of 2.16 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Sftp4, Sftpd, Pulmonary surfactant-associated protein D, PSP-D, SP-D, Lung surfactant protein D

8 Images

ELISA

Supplier Data

ELISA - Mouse SP-D ELISA Kit (AB240683)

sELISA

Supplier Data

Sandwich ELISA - Mouse SP-D ELISA Kit (AB240683)

Representative image of Mouse SP-D ELISA Kit (ab240683)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse SP-D ELISA Kit Capture Antibody

- 10X Mouse SP-D Detector Antibody

- 10X Mouse SP-D ELISA Kit Lyophilised Purified Protein (2 vials)

- Mouse SP-D ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Unknown

Sandwich ELISA - Mouse SP-D ELISA Kit (AB240683)

Interpolated concentrations of native SP-D in mouse serum and plasma samples. The concentrations of SP-D were measured in duplicates, interpolated from the SP-D standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 20%, plasma (citrate) 15%, plasma (EDTA) 30%, and plasma (heparin) 30%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SP-D concentration was determined to be 4172.67 pg/mL in serum, 5466.91 pg/mL in plasma (citrate), 3129.78 pg/mL in plasma (EDTA), and 2563.11 pg/mL in plasma (heparin).

sELISA

Unknown

Sandwich ELISA - Mouse SP-D ELISA Kit (AB240683)

Example of mouse SP-D standard curve in Sample Diluent NS. The SP-D standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse SP-D ELISA Kit (AB240683)

Interpolated concentrations of native SP-D in mouse lung extract based on a 50 μg/mL extract load. The concentrations of SP-D were measured in duplicate and interpolated from the SP-D standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SP-D concentration was determined to be 929.77 pg/mL in lung extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse SP-D ELISA Kit (AB240683)

Example of mouse SP-D standard curve in 1X Cell Extraction Buffer PTR. The SP-D standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse SP-D ELISA Kit (AB240683)

Interpolated concentrations of native SP-D in mouse cell culture supernatant samples. The concentrations of SP-D were measured in duplicates, interpolated from the SP-D standard curves and corrected for sample dilution. Undiluted samples are as follows : LPS treated lung supernatant 1%, and untreated lung supernatant 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SP-D concentration was determined to be 82.93 ng/mL in LPS treated lung supernatant and 26.10 ng/mL in untreated lung supernatant.

sELISA

Unknown

Sandwich ELISA - Mouse SP-D ELISA Kit (AB240683)

Mouse SP-D Standard Curve Comparison. Standard Curve comparison between mouse SP-D SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 2.16 pg/mL

Range

15.63 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse SP-D ELISA Kit ab240683 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse SP-D in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 2.16 pg/mL.

How the assay works

Mouse SP-D SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse SP-D ELISA Kit ab240683 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Surfactant protein D/SP-D antibody [EPR21774-52] - BSA and Azide free (Capture) ab245095
- Anti-Surfactant protein D/SP-D antibody [EPR21774-110] - BSA and Azide free (Detector) ab245095

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "103 - 108 %", "average": "= 104" }, { "sample": "Serum", "range": "94 - 102 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "89 - 110 %", "average": "= 98" }, { "sample": "Tissue Extracts", "range": "83 - 88 %", "average": "= 86" }, { "sample": "Heparin Plasma", "range": "99 - 115 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "89 - 102 %", "average": "= 100" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab240683-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB240683-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB240683-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB240683-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB240683-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB240683-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB240683-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse SP-D Capture Antibody", "number":"AB240683-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse SP-D Lyophilized recombinant Protein", "number":"AB240683-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB240683-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Mouse SP-D Detector Antibody", "number":"AB240683-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB240683-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB240683-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Surfactant protein D (SP-D) also known as protein D functions as an important component of the innate immune system. It weighs around 43 kDa and is primarily expressed in pulmonary alveoli where lung cells synthesize it. SP-D belongs to the collectin family and has a C-type lectin domain that allows binding to carbohydrate structures on microbial surfaces. This ability enables SP-D to interact directly with various pathogens and infected cells targeting them for clearance. Researchers often study SP-D with 'anti-surfactant' antibodies using methods like the ELISA protocol including versions like ELISA Sp and ELISA Denise.

Biological function summary

SP-D contributes to host defense by enhancing the uptake and elimination of pathogens by immune cells such as macrophages. Part of a larger protein complex SP-D can opsonize bacteria and viruses facilitating phagocytosis. Its role extends to modulating inflammation by affecting the production of pro-inflammatory cytokines. By maintaining homeostasis in surfactant composition it indirectly supports lung function and protects against lung injury. SP-D’s function involves communication and transmission of signals within the immune system making it integral to pulmonary immune processes.

Pathways

Surfactant protein D is involved in the regulation of the complement and coagulation cascades as well as the pathogen recognition pathway. It is closely related to other collectins such as surfactant protein A (SP-A) which share similar mechanisms in the immune response. SP-D's interaction within these pathways highlights its role in identifying and neutralizing pathogens. Through its connections with related proteins SP-D plays an important part in modulating immune responses ensuring effective pathogen elimination without excessive inflammation.

SP-D has been linked with respiratory diseases such as chronic obstructive pulmonary disease (COPD) and pulmonary fibrosis. These conditions may involve impaired SP-D function or expression levels potentially due to genetic factors or environmental influences. SP-D also interacts with proteins like SP-A in these diseases participating in lung immune responses. Disrupted regulation of SP-D can contribute to chronic inflammatory states in the lungs exacerbating disease progression and leading to compromised lung function. Understanding SP-D's role in these contexts offers potential therapeutic insights for targeting pathological inflammation.

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Product protocols

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Target data

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

See full target information Sftpd

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Publications (2)

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Species

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Paul R Stabach,Dominique Sims,Eduardo Gomez-Bañuelos,Sandra Zehentmeier,Kris Dammen-Brower,Andrew Bernhisel,Sophia Kujawski,Sam G Lopez,Michelle Petri,Daniel W Goldman,Ethan R Lester,Quan Le,Tayyaba Ishaq,Hana Kim,Shivani Srivastava,Deepika Kumar,Joao P Pereira,Kevin J Yarema,Fotios Koumpouras,Felipe Andrade,Demetrios T Braddock

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Xinyu Fan,Jiachen Shi,Ye Liu,Mengqiu Zhang,Min Lu,Ding Qu

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse SP-D ELISA Kit

ELISA - Mouse SP-D ELISA Kit (AB240683)

Sandwich ELISA - Mouse SP-D ELISA Kit (AB240683)

Sandwich ELISA - Mouse SP-D ELISA Kit (AB240683)

Sandwich ELISA - Mouse SP-D ELISA Kit (AB240683)

Sandwich ELISA - Mouse SP-D ELISA Kit (AB240683)

Sandwich ELISA - Mouse SP-D ELISA Kit (AB240683)

Sandwich ELISA - Mouse SP-D ELISA Kit (AB240683)

Sandwich ELISA - Mouse SP-D ELISA Kit (AB240683)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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