AB275104

Mouse ST6Gal1 ELISA Kit

Recombinant
SimpleStep
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Mouse ST6Gal1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse ST6Gal1 with a sensitivity of 27.53 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Siat1, St6gal1, ST6Gal I, Sialyltransferase 1, ST6GalI

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Sandwich ELISA - Mouse ST6Gal1 ELISA Kit (AB275104)

sELISA

Supplier Data

Sandwich ELISA - Mouse ST6Gal1 ELISA Kit (AB275104)

Interpolated concentrations of native ST6Gal1 in mouse serum and plasma samples.

The concentrations of ST6Gal1 were measured in duplicates, interpolated from the ST6Gal1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ST6Gal1 concentration was determined to be 5.53 ng/mL in serum, 7.66 ng/mL in plasma (citrate), 5.00 ng/mL in, plasma (EDTA), and 4.15 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Mouse ST6Gal1 ELISA Kit (AB275104)

Example of mouse ST6Gal1 standard curve in Sample Diluent NS.

The ST6Gal1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 27.53 pg/mL

Range

46.8 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse ST6Gal1 ELISA kit (ab275104) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse ST6Gal1 protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse ST6Gal1 with 27.53 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ST6GAL1 is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Literature has sited ST6GAL1 is expressed in mouse high endothelial cells of mesenteric lymph nodes and Peyer's patches. This kit recognizes the soluble form of mouse ST6Gal1 protein. Mouse ST6GAL1 shares 95%, 81%, 81%, 76% sequence homology with rat, human, primate, and cow, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]

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Recovery

[ { "sample": "Serum", "range": "92 - 106 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "83 - 114 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "97 - 117 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "89 - 95 %", "average": "= 92" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ST6GAL1 is an enzyme that belongs to the glycosyltransferase family specifically classified as a sialyltransferase. ST6GAL1 catalyzes the addition of sialic acid to glycoproteins and glycolipids impacting their function and stability. It has an approximate molecular mass of 45 kDa. ST6GAL1 is expressed in a variety of cell types including B lymphocytes and epithelial cells. Its expression can be observed on the cell surface where it plays important roles in cell-cell interactions.

Biological function summary

ST6GAL1 contributes to the mechanics of cell signaling and immune system modulation. ST6GAL1 is involved in the biosynthesis of sialylated glycoconjugates which affect cellular recognition and communication. This enzyme does not form part of a multi-protein complex but interacts with other proteins upon glycosylation events. Through its action ST6GAL1 impacts multiple cellular processes including inflammation regulation and pathogen recognition.

Pathways

ST6GAL1 plays roles in glycosylation and immune response mechanisms. It participates in the N-glycan biosynthesis pathway which influences protein folding and stability. Additionally ST6GAL1 impacts the sialylation pathway where it modifies the surface proteins for lymphocyte activation. Importantly ST6GAL1 interacts with proteins such as Siglecs which are involved in immune response modulation and cellular recognition.

ST6GAL1 is implicated in cancer progression and autoimmune diseases. In various cancers such as colon cancer altered ST6GAL1 expression affects metastatic potential and tumor cell survival. Additionally aberrant ST6GAL1 expression occurs in autoimmune diseases like lupus wherein it modulates immune cell activity. ST6GAL1 interacts with proteins like CD22 a sialic acid-binding lectin on B cells influencing disease pathogenesis and progression through immune system pathways.

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Product protocols

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Target data

Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.

See full target information St6gal1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse ST6Gal1 ELISA Kit

Sandwich ELISA - Mouse ST6Gal1 ELISA Kit (AB275104)

Sandwich ELISA - Mouse ST6Gal1 ELISA Kit (AB275104)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

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Assay Kits

Primary Antibodies

Product promise