AB202408

Mouse sTNF RI ELISA Kit (TNFRSF1A)

Recombinant
SimpleStep
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(2 Publications)

Mouse sTNF RI ELISA Kit (TNFRSF1A) is a single-wash 90-min Simplestep used to quantify Mouse sTNF RI (TNFRSF1A) with a sensitivity of 1.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD120a, Tnfr-1, Tnfr1, Tnfrsf1a, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor type I, p55, p60, TNF-R1, TNF-RI, TNFR-I

5 Images

Sandwich ELISA - Mouse sTNF RI ELISA Kit (TNFRSF1A) (AB202408)

sELISA

Unknown

Sandwich ELISA - Mouse sTNF RI ELISA Kit (TNFRSF1A) (AB202408)

Mouse TNFRSF1A standard curve comparison data.

Standard curve comparison between mouse TNFRSF1A SimpleStep ELISA^® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Mouse sTNF RI ELISA Kit (TNFRSF1A) (AB202408)

Interpolated concentrations of sTNF RI in mouse urine and L929 stimulated cell culture supernatant.

The concentrations of sTNF RI were measured in duplicate and interpolated from the sTNF RI standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RI concentration was determined to be 1,754 pg/mL in mouse urine, 538.3 pg/mL L929 stimulated cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse sTNF RI ELISA Kit (TNFRSF1A) (AB202408)

Example of sTNF RI standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse sTNF RI ELISA Kit (TNFRSF1A) (AB202408)

Comparison of secreted sTNF RI in unstimulated and PMA/PHA-stimulated L929 and J774A.1 Cells.

L929 and J774A.1 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. sTNF RI was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and J774A.1 and cell culture media. Measured values were interpolated from the sTNF RI Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed. sTNF RI is undetectable in media.

sELISA

Supplier Data

Sandwich ELISA - Mouse sTNF RI ELISA Kit (TNFRSF1A) (AB202408)

Interpolated concentrations of sTNF RI in mouse serum, plasma (citrate), and platelet poor plasma (EDTA).

The concentrations of sTNF RI were measured in duplicate and interpolated from the sTNF RI standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RI concentration was determined to be 3,427 pg/mL in mouse serum, 2,484 pg/mL in mouse plasma (Citrate), and 2,982 pg/mL in mouse plasma (EDTA).

Key facts

Detection method

Colorimetric

Sample types

Urine, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.2 pg/mL

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse sTNF RI ELISA Kit (TNFRSF1A) (ab202408) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of sTNF RI (TNFRSF1A) protein in cell culture supernatant, cit plasma, edta plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse sTNF RI (TNFRSF1A) with 1.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Tumor necrosis factor receptor superfamily member 1A (TNF RI) is a transmembrane protein with an extracellular domain that binds to TNF alpha. This extracellular domain can be proteolytically cleaved to make soluble TNF RI. The standard protein in this kit is soluble TNF RI and the capture and detector antibodies were raised against this region of TNF RI.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "13.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.5" } ]

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Recovery

[ { "sample": "Serum", "range": "107 - 118 %", "average": "= 113" }, { "sample": "EDTA Plasma", "range": "97 - 109 %", "average": "= 102" }, { "sample": "Urine", "range": "101 - 109 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "97 - 112 %", "average": "= 106" }, { "sample": "Cell culture media", "range": "94 - 95 %", "average": "= 95" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab202408-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB202408-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB202408-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB202408-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB202408-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB202408-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB202408-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse sTNF RI Capture Antibody", "number":"AB202408-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BC", "number":"AB202408-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse sTNF RI Lyophilized Recombinant Protein", "number":"AB202408-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse sTNF RI Detector Antibody", "number":"AB202408-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis (By similarity).

See full target information Tnfrsf1a

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 73:131-144 PubMed38091015

2023

Homer1 ameliorates ischemic stroke by inhibiting necroptosis-induced neuronal damage and neuroinflammation.

Applications

sELISA

Species

Horse

Weihao Lv,Qianqian Zhang,Yuanming Li,Dan Liu,Xiuquan Wu,Xin He,Yuanyuan Han,Xiaowei Fei,Lei Zhang,Zhou Fei

Diabetes, metabolic syndrome and obesity : targets and therapy 14:4679-4690 PubMed34916815

2021

Astragalus-Saffron-Rhubarb Mixture Delays the Progress of Diabetic Nephropathy in db/db Mice.

Applications

sELISA

Species

Horse

Xiao-Chun Zhou,Yao-Jun Liang,Long Qin,Guo-Hua Wei,Jian-Qin Wang

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse sTNF RI ELISA Kit (TNFRSF1A)

Sandwich ELISA - Mouse sTNF RI ELISA Kit (TNFRSF1A) (AB202408)

Sandwich ELISA - Mouse sTNF RI ELISA Kit (TNFRSF1A) (AB202408)

Sandwich ELISA - Mouse sTNF RI ELISA Kit (TNFRSF1A) (AB202408)

Sandwich ELISA - Mouse sTNF RI ELISA Kit (TNFRSF1A) (AB202408)

Sandwich ELISA - Mouse sTNF RI ELISA Kit (TNFRSF1A) (AB202408)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

Product promise