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AB285309

Mouse Superoxide Dismutase 1 ELISA Kit

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(5 Publications)

Mouse Superoxide Dismutase 1 ELISA Kit is a sandwich ELISA designed to quantify Mouse Superoxide Dismutase 1 with a sensitivity of 0.469 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.781 - 50 ng/mL

View Alternative Names

Superoxide dismutase [Cu-Zn], Hydrogen sulfide oxidase, Sod1

1 Images
Sandwich ELISA - Mouse Superoxide Dismutase 1 ELISA Kit (AB285309)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Superoxide Dismutase 1 ELISA Kit (AB285309)

Mouse Superoxide Dismutase 1 ELISA Kit.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Serum, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 0.469 ng/mL

Range

0.781 - 50 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Superoxide Dismutase 1 ELISA Kit ab285309 is a sandwich ELISA to measure Mouse Superoxide Dismutase 1 in serum, plasma, tissue homogenate, other biological fluids with a sensitivity of 0.469 ng/mL

How the assay works:

The density of color is proportional to the amount of SOD1 captured from the samples.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Superoxide Dismutase 1 ELISA Kit ab285309 protocol summary

1.Wash plate and add standards or samples to appropriate wells. Incubate
2. Add Biotin-detection antibody work solution to the wells. Incubate
3. Wash the plate
4. Add SABC working solution into each well. Incubate
5. Wash the plate
6. Add TMB substrate to the wells. Incubate
6. Add stop solutionand read at 450 nm
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8" } ]
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Recovery

[ { "sample": "Serum", "range": "86 - 102 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "89 - 100 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "93 - 103 %", "average": "= 99" } ]
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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285309-96Test", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"25X Wash buffer", "number":"AB285309-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB285309-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285309-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Standard", "number":"AB285309-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"Antibody dilution buffer", "number":"AB285309-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample / Standard Dilution Buffer", "number":"AB285309-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"SABC dilution buffer", "number":"AB285309-CMP05", "productcode":"" }, { "size":"1 x 120 µL", "name":"HRP-Streptavidin Conjugate (SABC)", "number":"AB285309-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution", "number":"AB285309-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"Biotin/Detection antibody (Concentrated)", "number":"AB285309-CMP02", "productcode":"" }, { "size":"5 x 1 Unit", "name":"Plate Sealer", "number":"AB285309-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Superoxide Dismutase 1 (SOD1) also known as Cu/Zn SOD or simply dismutase is an enzyme important for the detoxification of superoxide radicals. SOD1 catalyzes the conversion of two superoxide molecules into oxygen and hydrogen peroxide maintaining cellular redox balance. This enzyme typically exhibits a mass of approximately 32 kDa. SOD1 is expressed in the cytoplasm of cells throughout the body including tissues like liver kidney and brain.
Biological function summary

The enzyme functions as a homodimer with each subunit containing a copper and zinc ion. These metal ions are essential for the catalytic activity of SOD1 as the copper ion participates in electron transfer while the zinc ion provides structural stability. The enzyme protects cells from oxidative stress by neutralizing excess reactive oxygen species ensuring cellular health and functioning.

Pathways

SOD1 plays an important role in the cellular antioxidant defense system and is a part of the reactive oxygen species (ROS) metabolic pathway. It works in conjunction with catalase and glutathione peroxidase to limit oxidative damage within cells. The close interaction between these enzymes highlights the interdependence within the antioxidant defense network emphasizing their role in maintaining cellular homeostasis.

Mutations in the SOD1 gene are linked to familial Amyotrophic Lateral Sclerosis (ALS) a neurodegenerative condition. In ALS aberrant SOD1 proteins may lead to increased oxidative stress and motor neuron damage. Research also connects SOD1 to the pathogenesis of Alzheimer's disease where oxidative stress is a contributing factor. These associations underline SOD1's significance in neurodegenerative diseases highlighting potential therapeutic targets for interventions focused on oxidative stress management.
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Product protocols

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Target data

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
See full target information Sod1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 21:2770-2780 PubMed39512683

2024

LCN2 depletion aggravates sepsis-induced liver injury by regulating PTGS2-dependent ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Jiang,Zhi-Tian Jiang,Gang Zhao,Jing-Wen Cai,Jie Song,Jing Wang,Zhen Zhou,Qian Wang,Qi-Hua Ling

Allergologia et immunopathologia 52:21-28 PubMed39278847

2024

extract relieved sepsis-induced myocardial ferroptosis and inflammation by inhibiting lncRNA MIAT/TRAF6/NF-κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Shi,Xiaobo Yang,Hong Jiang,Shanxia Wu,Yan Hong,Wei Su,Xuan Wang

Frontiers in aging neuroscience 16:1365086 PubMed38464467

2024

(L.) Huds. and L. hydrosols mitigate aging related comorbidities in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Khadija Boualam,Hind Ibork,Zakaria Lahboub,Mansour Sobeh,Khalid Taghzouti

International journal of biological sciences 19:1831-1845 PubMed37063418

2023

Interaction between dual specificity phosphatase-1 and cullin-1 attenuates alcohol-related liver disease by restoring p62-mediated mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ruibing Li,Zhe Dai,Xiaoman Liu,Chunling Wang,Jia Huang,Ting Xin,Ying Tong,Yijin Wang

Theranostics 12:3196-3216 PubMed35547748

2022

Neuronal STAT3/HIF-1α/PTRF axis-mediated bioenergetic disturbance exacerbates cerebral ischemia-reperfusion injury via PLA2G4A.

Applications

Unspecified application

Species

Unspecified reactive species

Weili Jin,Jixing Zhao,Eryan Yang,Yunfei Wang,Qixue Wang,Ye Wu,Fei Tong,Yanli Tan,Junhu Zhou,Chunsheng Kang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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