AB202401

Mouse Survivin ELISA Kit (Birc5)

Recombinant
SimpleStep
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Mouse Survivin ELISA Kit (Birc5) is a single-wash 90-min Simplestep used to quantify Mouse Survivin (Birc5) with a sensitivity of 9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Api4, Iap4, Birc5, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, TIAP

3 Images

Sandwich ELISA - Mouse Survivin ELISA Kit (Birc5) (AB202401)

sELISA

Supplier Data

Sandwich ELISA - Mouse Survivin ELISA Kit (Birc5) (AB202401)

Nocodazole treatment of NIH/3T3 cells stimulates expression of Survivin.

NIH/3T3 cells were cultured in the absence or presence of 200 ng/mL nocodazole for 17 hours. The extracts of these cells (adjusted to the same protein concentration) were measured in dilution series (as indicated) with this kit. Interpolated concentrations of Survivin adjusted for sample dilution are graphed in pg of Survivin per mL of 1X Diluted extract (mean +/- SD, n = 2).

sELISA

Supplier Data

Sandwich ELISA - Mouse Survivin ELISA Kit (Birc5) (AB202401)

Example of Survivin standard curve prepared in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Survivin ELISA Kit (Birc5) (AB202401)

Interpolated concentrations of Survivin in NIH/3T3 cell extract samples.

The concentrations of Survivin were measured in duplicates, interpolated from the Survivin standard curve and corrected for sample dilution. Note that 1X Diluted extract samples were at 1 mg/mL. The interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 9 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Survivin ELISA Kit (Birc5) (ab202401) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Survivin (Birc5) protein in cell culture extracts, cell culture supernatant, cit plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Survivin (Birc5) with 9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Survivin is a multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Survivin is a component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Survivin acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and it participates in the organization of the center spindle by associating with polymerized microtubules. Survivin complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Survivin may counteract a default induction of apoptosis in G2/M phase. The acetylated form of Survivin represses STAT3 transactivation of target gene promoters. Survivin may play a role in neoplasia. Survivin may be an inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 of Survivin do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "NIH 3T3", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "13.1" }, { "reproducibilityType": "Intra", "sample": "NIH 3T3", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]

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Recovery

[ { "sample": "Serum", "range": "172.5 - 181.7 %", "average": "= 177" }, { "sample": "Heparin Plasma", "range": "105.9 - 121.4 %", "average": "= 112.3" }, { "sample": "Citrate plasma", "range": "85.4 - 98.3 %", "average": "= 90" }, { "sample": "Cell culture media", "range": "122.5 - 149.8 %", "average": "= 133.3" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab202401-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB202401-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB202401-CMP14", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB202401-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB202401-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB202401-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB202401-CMP13", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BC", "number":"AB202401-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Survivin (Birc5) Capture Antibody", "number":"AB202401-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BP", "number":"AB202401-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent NBP", "number":"AB202401-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Survivin (Birc5) Lyophilized Recombinant Protein", "number":"AB202401-CMP12", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB202401-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Survivin (Birc5) Detector Antibody", "number":"AB202401-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB202401-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Survivin also known as 'BIRC5 protein' is a small protein with a molecular weight of approximately 16.5 kDa. It belongs to the Inhibitor of Apoptosis (IAP) family carrying out its functions through inhibiting caspases and averting apoptosis. Survivin is expressed highly in embryonic tissues and various cancers but its expression in adult differentiated tissues is low. This selective expression pattern makes Survivin an attractive target for cancer research and therapeutic development.

Biological function summary

Survivin aids cell division and regulates apoptosis. It plays a major role as a chromosomal passenger protein functioning in concert with other members of the chromosomal passenger complex including Aurora B kinase INCENP and Borealin. During mitosis Survivin helps ensure proper chromosome alignment segregation and cytokinesis. Its ability to inhibit apoptosis allows it to enhance cell survival contributing to tumor progression when deregulated.

Pathways

Survivin is part of the cell cycle regulation and apoptosis pathways. It plays an integral role in the mitotic spindle checkpoint working closely with Aurora B kinase to regulate mitosis. Additionally the protein interfaces with the extrinsic apoptosis pathway interacting with members like XIAP to prevent caspase activation and cell death. These interactions reveal Survivin's dual function in promoting mitosis and regulating apoptosis which is essential for maintaining cellular homeostasis.

Survivin is highly connected to cancer and neurodegenerative disorders. Its overexpression links with cancers such as colorectal cancer where it promotes malignancy through inhibiting apoptosis and ensuring unchecked cell division. In neurodegenerative disorders abnormal regulation of Survivin could lead to insufficient cellular survival contributing to diseases like Alzheimer's. The relationship between Survivin and XIAP in cancer highlights its potential as a therapeutic target aiming to induce apoptosis in cancer cells by overcoming Survivin-mediated resistance.

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Product protocols

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Target data

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed : 25778398). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (By similarity). Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (By similarity). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (By similarity). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (By similarity). May counteract a default induction of apoptosis in G2/M phase (By similarity). The acetylated form represses STAT3 transactivation of target gene promoters (By similarity). May play a role in neoplasia. Inhibitor of CASP3 and CASP7 (By similarity). Essential for the maintenance of mitochondrial integrity and function (PubMed : 25778398).

See full target information Birc5

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Survivin ELISA Kit (Birc5)

Sandwich ELISA - Mouse Survivin ELISA Kit (Birc5) (AB202401)

Sandwich ELISA - Mouse Survivin ELISA Kit (Birc5) (AB202401)

Sandwich ELISA - Mouse Survivin ELISA Kit (Birc5) (AB202401)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Assay Kits

Assay Kits

Assay Kits

Product promise