Mouse Syndecan-1 ELISA kit

Product details

Mouse Syndecan-1 ELISA kit ab273165 is a sandwich ELISA to measure Mouse Syndecan-1 in serum, plasma, cell culture supernatant, other biological fluids with a sensitivity of 0.35 ng/ml.

How the assay works

A capture antibody highly specific for Syndecan-1 has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of Syndecan-1 in samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-Syndecan-1 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is then removed.The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of Syndecan-1 present in the samples and standards.The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of Syndecan-1 in any sample tested.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Syndecan-1 ELISA kit ab273165 protocol summary

1. Add 100 μl of sample and diluted standard.
2. Add 50 μl of biotinylated detection antibody. Incubate 2 hours at room temperature.<3> Wash three times then add 100 μl of Streptavidin-HRP.Incubate 30 mins at room temperature.
4.Wash three times then add 100 μl of ready-to-use TMB. Protect from light. Let the color develop for 5-15 mins.
5. Add 100 μl H2SO4 and read absorbance at 450 nm.