Mouse Syndecan-1 ELISA kit is a ELISA kit for the measurement of Mouse Syndecan-1 in Mouse in Cell culture supernatant, Other biological fluids, Plasma, Serum samples.
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- Science signaling 16:eadd65272023Skewing cPLAα activity toward oxoeicosanoid production promotes neutrophil N2 polarization, wound healing, and the response to sepsis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKenneth D Maus,Daniel J Stephenson,H Patrick Macknight,Ngoc T Vu,L Alexis Hoeferlin,Minjung Kim,Robert F Diegelmann,Xiujie Xie,Charles E ChalfantPubMed 37433004
Key facts
- Sample types
- Cell culture supernatant, Other biological fluids, Plasma, Serum
- Reactive species
- Mouse
- Range
- 0.5 - 16 ng/mL
- Assay time
- 2h 45m
- Sensitivity
- < 0.35 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix (PubMed:8163535). Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (By similarity). Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway (PubMed:8898217).
Alternative names
CD138, Synd-1, Synd1, Sdc1, Syndecan-1, SYND1
What's included?
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Mouse Syndecan-1 ELISA kit is a ELISA kit for the measurement of Mouse Syndecan-1 in Mouse in Cell culture supernatant, Other biological fluids, Plasma, Serum samples.
Key facts
- Sample types
- Cell culture supernatant, Other biological fluids, Plasma, Serum
- Reactive species
- Mouse
- Range
- 0.5 - 16 ng/mL
- Assay time
- 2h 45m
- Sensitivity
- < 0.35 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Various | n 0 | mean - | SD - | C.V. 6.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Various | n 0 | mean - | SD - | C.V. 7.6 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Syndecan-1 ELISA kit (ab273165) is for the in-vitro qualitative and quantitative determination of Syndecan-1 in cell culture supernatants, buffered solutions or mouse serum, plasma, or other body fluids. This assay will recognize both natural and recombinant mouse Syndecan-1.
A capture antibody highly specific for Syndecan-1 has been coated to the wells of the microtiter strip plate provided during manufacture.
Binding of Syndecan-1 in samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-Syndecan-1 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is then removed.
The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing.
A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of Syndecan-1 present in the samples and standards.
The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of Syndecan-1 in any sample tested.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Syndecan-1 also known as CD138 is a transmembrane heparan sulfate proteoglycan with a molecular weight of approximately 30 to 80 kDa. The size variation arises from differences in its glycosaminoglycan chains. This protein resides predominantly on the surface of epithelial cells and plasma cells. Researchers commonly use the CD138 marker to identify these cells in biological samples. It plays a significant role in cell-cell and cell-matrix interactions acting as a co-receptor for a variety of growth factors cytokines and extracellular matrix proteins.
- Biological function summary
Syndecan-1 participates in tissue repair inflammation and cell signaling ensuring epithelial integrity and communication. Syndecan-1 influences cell behavior by interacting with other components of the extracellular matrix and different membrane receptors. It is also involved in forming and regulating proteoglycan complexes at the cell surface. These complex interactions facilitate the modulation of cell proliferation migration and differentiation which are fundamental to maintaining tissue architecture and function.
- Pathways
Syndecan-1 actively contributes to both the Wnt and FGF signaling pathways. Through its interaction with proteins such as miR-15 syndecan-1 modulates cellular responses essential for developmental processes and tissue homeostasis. The connection with these pathways allows syndecan-1 to integrate and relay extracellular signals impacting gene expression and cellular behavior. Its relationship with other proteins like A38 as a co-receptor emphasizes its role in finely tuning signaling cascades within cellular environments.
- Associated diseases and disorders
Syndecan-1 shows a connection to multiple myeloma and breast cancer. The overexpression of syndecan-1 in these conditions disrupts normal cellular functions and promotes tumorigenesis by aiding in the tumor cell's adhesion migration and invasion. Its interaction with proteins such as perCP further demonstrates its role in disease progression providing potential as a therapeutic target. These relationships make syndecan-1 an important focus for research in oncology and other fields of disease study.
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ELISA - Mouse Syndecan-1 ELISA kit (ab273165)
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.Example of Mouse Syndecan-1 ELISA kit standard curve.
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Product protocols
- Visit the general protocols
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