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AB206312

Mouse TACI ELISA Kit (TNFRSF13B)

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Mouse TACI ELISA Kit (TNFRSF13B) is a single-wash 90-min Simplestep used to quantify Mouse TACI (TNFRSF13B) with a sensitivity of 4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD267, Taci, Tnfrsf13b, Tumor necrosis factor receptor superfamily member 13B, Transmembrane activator and CAML interactor

4 Images
Sandwich ELISA - Mouse TACI ELISA Kit (TNFRSF13B) (AB206312)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TACI ELISA Kit (TNFRSF13B) (AB206312)

Example of mouse TACI standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TACI ELISA Kit (TNFRSF13B) (AB206312)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TACI ELISA Kit (TNFRSF13B) (AB206312)

Interpolated concentrations of TACI in mouse spleen and A20 cell extracts.

The concentrations of TACI were measured in duplicate and interpolated from the TACI standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TACI concentration was determined to be 5174.4 pg/mL in 4 mg/mL mouse spleen extract and 23 ng/mL in 150 μg/mL A20 cell extract.

Sandwich ELISA - Mouse TACI ELISA Kit (TNFRSF13B) (AB206312)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TACI ELISA Kit (TNFRSF13B) (AB206312)

Interpolated concentrations of TACI in mouse serum, mouse plasma (citrate), and mouse plasma (EDTA).

The concentrations of TACI were measured in duplicate and interpolated from the TACI standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TACI concentration was determined to be 801.6 pg/mL in neat mouse serum,1744.4 pg/mL in neat mouse plasma (Citrate), 1766.4 pg/mL in neat mouse plasma (EDTA), and 16.2 ng/mL in neat A20 Cell Culture Supernatant.

Sandwich ELISA - Mouse TACI ELISA Kit (TNFRSF13B) (AB206312)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TACI ELISA Kit (TNFRSF13B) (AB206312)

Example of mouse TACI standard curve in 1X Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 4 pg/mL

Range

15.83 - 1013 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse TACI ELISA Kit (TNFRSF13B) (ab206312) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TACI (TNFRSF13B) protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TACI (TNFRSF13B) with 4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Transmembrane activator and CAML interactor (TACI), also known as TNF receptor super family member 13B (TNFRSF13B), is a transmembrane receptor expressed primarily in mature B cells and is a regulator of B-cell homeostasis and immune response. Specifically, TACI binds both a proliferation-inducing ligand (APRIL) and B cell activating factor of the tumor necrosis factor family (BAFF) similarly with high affinity.Taci−/−mice have demonstrated that inadequate TACI signaling promotes the development of autoimmune diseases. This assay recognizesthe extracellular N-terminus of TACI.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Mouse Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" }, { "reproducibilityType": "Intra", "sample": "Mouse Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "100 - 110 %", "average": "= 106" }, { "sample": "Serum", "range": "90 - 96 %", "average": "= 94" }, { "sample": "Cell culture extracts", "range": "99 - 107 %", "average": "= 103" }, { "sample": "Tissue Extracts", "range": "100 - 112 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "95 - 98 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "90 - 97 %", "average": "= 94" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab206312-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB206312-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse TACI Lyophilized Recombinant Protein", "number":"AB206312-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB206312-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB206312-CMP13", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB206312-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse TACI Capture Antibody", "number":"AB206312-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB206312-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB206312-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB206312-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB206312-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse TACI Detector Antibody", "number":"AB206312-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB206312-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transmembrane Activator and CAML Interactor known as TACI also goes by the names TNFRSF13B and CD267. TACI is a receptor with a molecular weight of approximately 33 kDa. It is expressed on B cells a type of lymphocyte and to some extent on activated T cells. This receptor plays a part in the immune system helping manage the production and function of certain antibodies.
Biological function summary

TACI involves the regulation of B cell function and survival. It binds to two ligands APRIL and BAFF and TACI is not part of any larger complex. Upon ligand binding TACI can promote antibody class-switching which adjusts the type of antibody that B cells produce. This action supports the immune response by allowing the body to adapt to different pathogens effectively.

Pathways

Immune responses involve TACI in the BAFF-APRIL signaling pathway. The interplay between TACI and its ligands influences the NF-kB signaling cascade leading to B cell proliferation and survival. TACI interacts with other proteins such as BCMA and BAFF-R which are important in regulating B cell homeostasis and function ensuring balanced and effective immune responses.

Aberrant TACI function contributes to conditions like Common Variable Immunodeficiency (CVID) and Systemic Lupus Erythematosus (SLE). In CVID mutations in the TACI gene may lead to impaired antibody production resulting in frequent infections. In the context of SLE TACI's interactions with BAFF and APRIL can contribute to the overactivation of B cells causing autoimmune characteristics of the disease. TACI's role in these conditions makes it a focus for studying therapeutic interventions.
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Product protocols

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Target data

Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T-cell function and the regulation of humoral immunity (By similarity).
See full target information Tnfrsf13b
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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