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AB213474

Mouse TARC ELISA Kit (CCL17)

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Mouse TARC ELISA Kit (CCL17) is a single-wash 90-min Simplestep used to quantify Mouse TARC (CCL17) with a sensitivity of 2.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Tarc, Ccl17, C-C motif chemokine 17, ABCD-2, CC chemokine TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine

8 Images
ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)
  • ELISA

Supplier Data

ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)
Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)

Example of mouse TARC standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)
  • sELISA

Unknown

Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)

Mouse TARC standard curve comparison data.

Standard curve comparison between mouse TARC SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)

Example of mouse TARC standard curve in Sample Diluent 50BS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)

Interpolated concentrations of native TARC in mouse splenocyte and lung supernatant samples.

The concentrations of TARC were measured in duplicates, interpolated from the TARC standard curves and corrected for sample dilution. Undiluted samples are as follows : splenocyte supernatant 50%, and lung supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TARC concentration was determined to be 25 pg/mL in splenocyte supernatant and 756 pg/mL in lung supernatant.

Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)

Interpolated concentrations of native TARC in mouse serum and plasma samples.

The concentrations of TARC were measured in duplicates, interpolated from the TARC standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TARC concentration was determined to be 91 pg/mL in serum, 25.4 pg/mL in plasma (citrate), 23.0 pg/mL in plasma (EDTA) and 25.4 pg/mL in plasma (heparin).

Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)

Example of mouse TARC standard curve in Sample Diluent 5BS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (AB213474)

Interpolated concentrations of spiked TARC in mouse serum and plasma samples.

The concentrations of TARC were measured in duplicates, interpolated from the TARC standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.5 pg/mL

Range

7.8 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse TARC ELISA Kit (CCL17) ab213474 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse TARC (CCL17)in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 2.5 pg/mL.

How the assay works

Mouse TARC (CCL17)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse TARC ELISA Kit (CCL17) ab213474 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

TARC (also known as C-C motif chemokine 17) is a chemokine that functions through chemokine receptors CCR4 and CCR8. TARC is expressed in the thymus as well as by dendritic cells, keratinocytes and bronchial epithelial cells. The standard is affinity purified.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.6" } ]
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Recovery

[ { "sample": "Serum", "range": "81 - 92 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "103 - 112 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "76 - 84 %", "average": "= 81" }, { "sample": "Citrate plasma", "range": "95 - 107 %", "average": "= 101" }, { "sample": "Cell culture media", "range": "93 - 101 %", "average": "= 98" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213474-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB213474-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB213474-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB213474-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB213474-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB213474-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB213474-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse TARC Lyophilized Recombinant Protein", "number":"AB213474-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB213474-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse TARC Capture Antibody", "number":"AB213474-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent NBS", "number":"AB213474-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse TARC Detector Antibody", "number":"AB213474-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TARC also known as CCL17 is a chemokine with a molecular mass of approximately 8 kDa. It is mainly expressed in the thymus but also found in the lung liver and lymph nodes. CCL17 plays an important role in immune response by signaling through the CCR4 receptor. It functions as a ligand that guides the movement of T-helper 2 (Th2) cells regulatory T cells (Tregs) and other immune cells to sites of inflammation.
Biological function summary

CCL17 acts as a chemotactic agent attracting specific immune cells especially T lymphocytes to inflammatory regions. This protein does not operate as part of a larger protein complex but engages directly by binding to receptors on the target immune cells. Localized expression of CCL17 increases in conditions where enhanced Th2 cell response is needed such as allergic or autoimmune responses.

Pathways

CCL17 plays a significant role in the cell signaling pathway called the chemokine signaling pathway. Through this pathway CCL17 interacts with the CCR4 receptor which helps mediate immune cell migration and adhesion. This interaction is critical for establishing the adaptive immune response. CCL17 and CCR4 support the trafficking of Th2 cells during immune reactions and pathogen defense.

CCL17 associates with asthma and atopic dermatitis where Th2 cell recruitment and activity are heightened. In asthma increased CCL17 levels exacerbate inflammatory response in the airways. In atopic dermatitis CCL17 presence corresponds with the intensity of the skin inflammation. These conditions are linked to the overactivity of Th2 cytokines such as IL-4 (H4L3) which collaborate with CCL17 to drive allergic responses.
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Product protocols

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Target data

Chemokine, which displays chemotactic activity for T lymphocytes, preferentially Th2 cells, but not monocytes or granulocytes. Therefore plays an important role in a wide range of inflammatory and immunological processes (PubMed : 10508243, PubMed : 10508268). Acts by binding to CCR4 at T-cell surface (By similarity). Mediates GM-CSF/CSF2-driven pain and inflammation (PubMed : 27525438). In the brain, required to maintain the typical, highly branched morphology of hippocampal microglia under homeostatic conditions. May be important for the appropriate adaptation of microglial morphology and synaptic plasticity to acute lipopolysaccharide (LPS)-induced neuroinflammation (PubMed : 30277599). Plays a role in wound healing, mainly by inducing fibroblast migration into the wound (PubMed : 21521373).
See full target information Ccl17
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websiteProtocolBooklet
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