Mouse TARC ELISA Kit (CCL17) is a single-wash 90-min Simplestep used to quantify Mouse TARC (CCL17) with a sensitivity of 2.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 7.8 - 500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2.5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Chemokine, which displays chemotactic activity for T lymphocytes, preferentially Th2 cells, but not monocytes or granulocytes. Therefore plays an important role in a wide range of inflammatory and immunological processes (PubMed:10508243, PubMed:10508268). Acts by binding to CCR4 at T-cell surface (By similarity). Mediates GM-CSF/CSF2-driven pain and inflammation (PubMed:27525438). In the brain, required to maintain the typical, highly branched morphology of hippocampal microglia under homeostatic conditions. May be important for the appropriate adaptation of microglial morphology and synaptic plasticity to acute lipopolysaccharide (LPS)-induced neuroinflammation (PubMed:30277599). Plays a role in wound healing, mainly by inducing fibroblast migration into the wound (PubMed:21521373).
Alternative names
Tarc, Ccl17, C-C motif chemokine 17, ABCD-2, CC chemokine TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine
What's included?
Recommended products
Mouse TARC ELISA Kit (CCL17) is a single-wash 90-min Simplestep used to quantify Mouse TARC (CCL17) with a sensitivity of 2.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 7.8 - 500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2.5 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 5 | mean - | SD - | C.V. 9.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 6.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 86 | Range 81 - 92 % |
Sample type EDTA Plasma | Average % = 107 | Range 103 - 112 % |
Sample type Heparin Plasma | Average % = 81 | Range 76 - 84 % |
Sample type Citrate plasma | Average % = 101 | Range 95 - 107 % |
Sample type Cell culture media | Average % = 98 | Range 93 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse TARC ELISA Kit (CCL17) (ab213474) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TARC (CCL17) protein in cell culture supernatant, cit plasma, hep plasma, serum, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TARC (CCL17) with 2.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
TARC (also known as C-C motif chemokine 17) is a chemokine that functions through chemokine receptors CCR4 and CCR8. TARC is expressed in the thymus as well as by dendritic cells, keratinocytes and bronchial epithelial cells.The standard is affinity purified.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TARC also known as CCL17 is a chemokine with a molecular mass of approximately 8 kDa. It is mainly expressed in the thymus but also found in the lung liver and lymph nodes. CCL17 plays an important role in immune response by signaling through the CCR4 receptor. It functions as a ligand that guides the movement of T-helper 2 (Th2) cells regulatory T cells (Tregs) and other immune cells to sites of inflammation.
- Biological function summary
CCL17 acts as a chemotactic agent attracting specific immune cells especially T lymphocytes to inflammatory regions. This protein does not operate as part of a larger protein complex but engages directly by binding to receptors on the target immune cells. Localized expression of CCL17 increases in conditions where enhanced Th2 cell response is needed such as allergic or autoimmune responses.
- Pathways
CCL17 plays a significant role in the cell signaling pathway called the chemokine signaling pathway. Through this pathway CCL17 interacts with the CCR4 receptor which helps mediate immune cell migration and adhesion. This interaction is critical for establishing the adaptive immune response. CCL17 and CCR4 support the trafficking of Th2 cells during immune reactions and pathogen defense.
- Associated diseases and disorders
CCL17 associates with asthma and atopic dermatitis where Th2 cell recruitment and activity are heightened. In asthma increased CCL17 levels exacerbate inflammatory response in the airways. In atopic dermatitis CCL17 presence corresponds with the intensity of the skin inflammation. These conditions are linked to the overactivity of Th2 cytokines such as IL-4 (H4L3) which collaborate with CCL17 to drive allergic responses.
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7 product images
Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (ab213474)
Mouse TARC standard curve comparison data.
Standard curve comparison between mouse TARC SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (ab213474)
Example of mouse TARC standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (ab213474)
Example of mouse TARC standard curve in Sample Diluent 50BS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (ab213474)
Example of mouse TARC standard curve in Sample Diluent 5BS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (ab213474)
Interpolated concentrations of spiked TARC in mouse serum and plasma samples.
The concentrations of TARC were measured in duplicates, interpolated from the TARC standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (ab213474)
Interpolated concentrations of native TARC in mouse serum and plasma samples.
The concentrations of TARC were measured in duplicates, interpolated from the TARC standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TARC concentration was determined to be 91 pg/mL in serum, 25.4 pg/mL in plasma (citrate), 23.0 pg/mL in plasma (EDTA) and 25.4 pg/mL in plasma (heparin).
Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (ab213474)
Interpolated concentrations of native TARC in mouse splenocyte and lung supernatant samples.
The concentrations of TARC were measured in duplicates, interpolated from the TARC standard curves and corrected for sample dilution. Undiluted samples are as follows: splenocyte supernatant 50%, and lung supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TARC concentration was determined to be 25 pg/mL in splenocyte supernatant and 756 pg/mL in lung supernatant.
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