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AB155460

Mouse TCA-3 ELISA Kit (CCL1)

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Mouse TCA-3 ELISA Kit (CCL1) is a Sandwich (quantitative) ELISA for the measurement of Mouse TCA-3 (CCL1) in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Scya1, Tca3, Ccl1, C-C motif chemokine 1, P500, SIS-epsilon, Small-inducible cytokine A1, T-cell activation protein 3, TCA-3, TCA3

2 Images
Sandwich ELISA - Mouse TCA-3 ELISA Kit (CCL1) (AB155460)
  • sELISA

Unknown

Sandwich ELISA - Mouse TCA-3 ELISA Kit (CCL1) (AB155460)

Representative standard curve using ab155460 - Assay Diluent D.

Sandwich ELISA - Mouse TCA-3 ELISA Kit (CCL1) (AB155460)
  • sELISA

Unknown

Sandwich ELISA - Mouse TCA-3 ELISA Kit (CCL1) (AB155460)

Representative standard curve using ab155460 - Assay Diluent B.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 30 pg/mL

Range

0.033 - 8 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TCA-3 (CCL1) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of TCA-3 (CCL1) in mouse serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse TCA-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and TCA-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TCA-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and colour develops in proportion to the amount of TCA-3 bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "77 - 103 %", "average": "= 91.6" }, { "sample": "Serum", "range": "119 - 135 %", "average": "= 127.7" }, { "sample": "Plasma", "range": "109 - 139 %", "average": "= 126.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155460-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155460-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155460-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB155460-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155460-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TCA-3 Microplate (12 x 8 wells)", "number":"AB155460-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse TCA-3 Standard (lyophilized)", "number":"AB155460-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse TCA-3", "number":"AB155460-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin Concentrate", "number":"AB155460-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155460-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCA-3 also known as CCL1 is a chemokine protein with an approximate molecular weight of 10 kDa. It belongs to the CC chemokine family and functions as a signaling molecule in immune responses. TCA-3 exhibits high expression in activated T lymphocytes macrophages and dendritic cells. This protein plays a role in the regulation of immune cell trafficking by interacting with specific G-protein-coupled receptors on the surfaces of target cells influencing cell movement towards the sites of inflammation or injury.
Biological function summary

TCA-3 impacts the immune system by contributing to cytokine receptor-mediated signaling pathways. It is involved in promoting T cell activation and differentiation facilitating immune surveillance and response. TCA-3 operates not as part of a complex but mainly influences cell behavior through its receptor CCR8. This interaction modulates key functions such as cell migration and immune response regulation.

Pathways

TCA-3 participates significantly in the chemokine signaling pathway and the innate immune response. It is vital in mediating inflammatory responses allowing TCA-3 to direct the movement of cells to inflammation sites. TCA-3 has functional links with proteins like CCR8 its receptor enabling T cells to respond selectively to TCA-3 signals. These associations highlight its role in shaping the body's ability to respond to immune challenges.

The dysregulation of TCA-3 has implications in conditions like rheumatoid arthritis and certain allergic reactions. Elevated levels of TCA-3 can lead to excessive recruitment of inflammatory cells exacerbating disease symptoms. Through its receptor CCR8 TCA-3 may influence disease progression and severity by altering immune cell behavior. Understanding TCA-3's role and connections in these disorders can inform therapeutic strategies targeting immune and inflammatory processes.
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Product protocols

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Target data

Cytokine that is chemotactic for neutrophils.
See full target information Ccl1
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websiteProtocolBooklet
en
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