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AB100743

Mouse TECK ELISA Kit

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Mouse TECK ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse TECK in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Scya25, Teck, Ccl25, C-C motif chemokine 25, Chemokine TECK, Small-inducible cytokine A25, Thymus-expressed chemokine

2 Images
Sandwich ELISA - Mouse TECK ELISA Kit (AB100743)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TECK ELISA Kit (AB100743)

Representative Standard Curve using ab100743

Sandwich ELISA - Mouse TECK ELISA Kit (AB100743)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TECK ELISA Kit (AB100743)

Representative Standard Curve using ab100743

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 15 pg/mL

Range

20.5 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TECK Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TECK in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse TECK coated on a 96- well plate. Standards and samples are pipetted into the wells and TECK present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TECK antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TECK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 107 %", "average": "= 97.42" }, { "sample": "Serum", "range": "82 - 105 %", "average": "= 94.26" }, { "sample": "Plasma", "range": "80 - 106 %", "average": "= 92.68" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100743-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100743-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100743-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TECK Microplate (12 x 8 wells)", "number":"AB100743-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"120X HRP-Streptavidin Concentrate", "number":"AB100743-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100743-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100743-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100743-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse TECK", "number":"AB100743-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse TECK Standard (lyophilized)", "number":"AB100743-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TECK also known as thymus-expressed chemokine or CCL25 is a small cytokine with a molecular mass of approximately 15 kDa. It belongs to the CC chemokine family. TECK is highly expressed in the thymus and small intestine where it plays a significant role in immune system regulation. Its mechanical function mainly involves binding to the CCR9 receptor on cells prompting chemotaxis of T cells and other leukocytes to specific tissues. This function facilitates thymocyte migration and homing of T cells to the gut.
Biological function summary

TECK influences immune response regulation beyond its role in cell migration. It participates in the development of the gut-associated lymphoid tissues. Although TECK is not part of a larger protein complex its interaction with CCR9 is essential for T cell localization and differentiation impacting immune surveillance and response. This specificity to certain tissues marks TECK as a critical player in maintaining immune homeostasis in the gut and thymus.

Pathways

TECK is significantly involved in the chemokine signaling pathway essential for lymphocyte trafficking and immune cell positioning. It also plays a part in the G protein-coupled receptor (GPCR) signaling pathway through its interaction with CCR9 aligning with proteins such as SDF-1 and its receptor CXCR4 which have overlapping functions in managing cell migration. These pathways highlight the importance of TECK in orchestrating immune activities and cell positioning within specific tissues.

TECK has shown associations with inflammatory diseases and certain cancers. Inflammatory bowel disease (IBD) for instance illustrates a context where TECK's regulation of immune cell migration can become dysregulated contributing to disease pathology. Similarly TECK's role in T cell localization implicates it in some cancers like small intestine adenocarcinoma where its interaction with CCR9 can influence cancer cell movement and metastasis. Understanding TECK in these contexts helps in identifying potential therapeutic targets or biomarkers for disease management.
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Product protocols

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Target data

Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
See full target information Ccl25
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