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AB269560

Mouse TECK ELISA Kit (CCL25)

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Mouse TECK ELISA Kit (CCL25) is a single-wash 90-min Simplestep used to quantify Mouse TECK (CCL25) with a sensitivity of 7.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Scya25, Teck, Ccl25, C-C motif chemokine 25, Chemokine TECK, Small-inducible cytokine A25, Thymus-expressed chemokine

2 Images
Sandwich ELISA - Mouse TECK ELISA Kit (CCL25) (AB269560)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TECK ELISA Kit (CCL25) (AB269560)

Interpolated concentrations of native TECK in mouse thymus extract samples based on a 75 μg/mL extract load.

The concentrations of TECK were measured in duplicate and interpolated from the TECK standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TECK concentration was determined to be 658 pg/mL in thymus extract.

Sandwich ELISA - Mouse TECK ELISA Kit (CCL25) (AB269560)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TECK ELISA Kit (CCL25) (AB269560)

Example of mouse TECK standard curve in 1X Cell Extraction Buffer PTR.

The TECK standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 7.4 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse TECK ELISA Kit (CCL25) (ab269560) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TECK (CCL25) protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TECK (CCL25) with 7.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Mouse TECK (CCL25) is a CC chemokine produced by stromal cells in the thymus and epithelial cells of the small intestine. TECK is involved in human T-cell development inducing chemoattraction through CCR9 on immature pre-T cells and thymocytes. TECK induces the homing of several lymphocyte populations to the small intestine, including integrin a4b7+ gamma/delta T cells, Integrin aEb7+ CD8+ T cells, and IgA-producing plasma cells. In human cancer, TECK promotes the metastasis of melanoma cells, migration and invasion of some breast carcinomas, and mesenchymal stromal cells in multiple myelomas. TECK attracts CCR9+ monocytes and macrophages in rheumatoid arthritis and CCR9+ macrophages in atherosclerosis. Mouse TECK shares 50% ,81% and 61 % sequence homology with mouse, rat and cow, respectively

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "89 - 114 %", "average": "= 99" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab269560-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB269560-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB269560-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB269560-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse TECK (CCL25) Capture Antibody", "number":"AB269560-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse TECK (CCL25) Lyophilized Recombinant Protein", "number":"AB269560-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB269560-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB269560-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse TECK (CCL25) Detector Antibody", "number":"AB269560-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB269560-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB269560-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB269560-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL25 also known as TECK stands as a chemokine with a central role in immune cell attraction. It weighs approximately 15 kDa and is mainly expressed in the thymus and small intestine. This chemokine's expression is associated with its activity in guiding the movement of various immune cells like lymphocytes. Additionally CCL25 interacts with its receptor CCR9 facilitating important cellular signaling necessary for immune responses.
Biological function summary

This chemokine aids in the migration and homing of T-cell subsets particularly to the gut and small intestine hinting at its role in mucosal immunity. CCL25 does not belong to a larger multiprotein complex but it plays a standalone role in controlling immune surveillance and response. Its involvement contributes to the precise regulation of immune cell localization especially under inflammatory conditions.

Pathways

CCL25 participates significantly in the chemokine signaling pathway and the integrin-mediated adhesion pathway. It cooperates with proteins like CCR9 which is its receptor to activate downstream signaling cascades necessary for immune cell trafficking and adhesion. Through these pathways CCL25 ensures correct immune cell distribution throughout the body supporting effective immune surveillance and response.

CCL25 is associated with inflammatory bowel disease (IBD) and celiac disease. In these conditions CCL25 together with its receptor CCR9 contributes to the recruitment of lymphocytes to the inflamed gut. This recruitment exacerbates inflammation and may also link to other inflammatory proteins like TNF-alpha fostering an environment of sustained inflammation. Understanding CCL25's role in such diseases could inform therapeutic strategies aimed at modulating immune cell trafficking.
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Product protocols

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Target data

Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
See full target information Ccl25
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websiteProtocolBooklet
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Product promise

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