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AB253228

Mouse TFF3 ELISA Kit

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Mouse TFF3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse TFF3 with a sensitivity of 2.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Itf, Tff3, Trefoil factor 3, Intestinal trefoil factor, mITF

2 Images
Sandwich ELISA - Mouse TFF3 ELISA Kit (AB253228)
  • sELISA

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Sandwich ELISA - Mouse TFF3 ELISA Kit (AB253228)

Interpolated concentrations of native TFF3 in mouse plasma and cell samples.

The concentrations of TFF3 were measured in duplicates, interpolated from the TFF3 standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (citrate) 20%, plasma (EDTA) 20%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFF3 concentration was determined to be 1599.11 pg/mL plasma (citrate) in 2601.71 pg/mL in plasma (citrate), and 2822.63 pg/mL in plasma (heparin).

Sandwich ELISA - Mouse TFF3 ELISA Kit (AB253228)
  • sELISA

Unknown

Sandwich ELISA - Mouse TFF3 ELISA Kit (AB253228)

Example of mouse TFF3 standard curve in Sample Diluent NS.

The TFF3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 2.9 pg/mL

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse TFF3 ELISA Kit (ab253228) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TFF3 protein in cit plasma, edta plasma, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TFF3 with 2.9 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Trefoil factor 3 is a protein that is encoded by the TFF3 gene. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to anti-apoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. TFF3 is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies. Mouse TFF3 shares 85.2%, 94.8%, 82.5%, and 72.9% sequence homology with human, rat, bovine, and primate respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "82 - 85 %", "average": "= 84" }, { "sample": "EDTA Plasma", "range": "82 - 90 %", "average": "= 87" }, { "sample": "Heparin Plasma", "range": "85 - 99 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "87 - 101 %", "average": "= 95" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Trefoil Factor 3 (TFF3) also known as intestinal trefoil factor is a small peptide with a molecular mass around 7 kDa. This protein is characterized by the presence of a trefoil domain which consists of a stable compact structure important for its function. TFF3 has significant expression in the gastrointestinal tract notably in the colon and small intestine where it acts to maintain the integrity of the mucosal barrier. It can also be found in other tissues but its concentrations vary depending on factors like tissue type and health status.
Biological function summary

TFF3 plays an important role in protecting and repairing epithelial tissues particularly those lining the gastrointestinal tract. This protein promotes epithelial restitution by aiding cell migration and inhibits apoptosis contributing to tissue healing processes. While TFF3 does not form part of a large protein complex it often interacts with mucins specifically mucin 2 to bolster the protective mucus layer which is essential for shielding epithelial cells.

Pathways

Trefoil Factor 3 is notably involved in pathways associated with mucosal immune response and inflammation. A significant pathway involving TFF3 is the mucosal defense pathway where it works alongside other mucins and proteins like epidermal growth factor (EGF) to mediate regeneration and defense of epithelial tissues after injury. It also engages in the Wnt signaling pathway where it affects cell proliferation and migration critical for maintaining epithelial homeostasis.

TFF3 has links to inflammatory bowel disease (IBD) and colorectal cancer. In IBD its expression levels may be altered affecting the ability of the intestine to protect itself and heal contributing to the disease's progression. In colorectal cancer TFF3 can exhibit upregulated expression linking it to tumor growth and metastasis. Proteins such as beta-catenin in the Wnt signaling pathway which are associated with cell cycle regulation have connections with TFF3 highlighting its role in cancerous transformations.
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Product protocols

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Target data

Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
See full target information Tff3
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websiteProtocolBooklet
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