Mouse TFPI ELISA Kit
- Recombinant
- SimpleStep
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(1 Publication)
Mouse TFPI ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse TFPI with a sensitivity of 1.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Tissue factor pathway inhibitor, TFPI, Extrinsic pathway inhibitor, Lipoprotein-associated coagulation inhibitor, EPI, LACI, Tfpi
- sELISA
Supplier Data
Sandwich ELISA - Mouse TFPI ELISA Kit (AB217776)
Interpolated concentrations of native TFPI in mouse serum, and plasma samples.
The concentrations of TFPI were measured in duplicates, interpolated from the TFPI standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 100, plasma (citrate) 1 : 400, plasma (EDTA) 1 : 400 and plasma (heparin) 1 : 400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFPI concentration was determined to be 57.10 ng/mL in neat serum, 241.24 ng/mL in neat plasma (citrate), 259.59 ng/mL in neat plasma (EDTA) and 303.08 ng/mL in neat plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Mouse TFPI ELISA Kit (AB217776)
Interpolated concentrations of native and spiked TFPI in mouse urine and spiked TFPI in Cell Culture Media samples.
The concentrations of TFPI were measured in duplicates, interpolated from the TFPI standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 50%, and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFPI concentration was determined to be 120.64 pg/mL in native, neat urine, 1,353.96 pg/mL in spiked, neat urine and 1,130.58 pg/mL in spiked, neat cell culture media.
- sELISA
Supplier Data
Sandwich ELISA - Mouse TFPI ELISA Kit (AB217776)
Example of mouse TFPI standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse TFPI ELISA Kit (AB217776)
Interpolated concentrations of native TFPI in mouse heart and liver tissue extract samples based on a 500 μg/mL extract load.
The concentrations of TFPI were measured in duplicate and interpolated from the TFPI standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFPI concentration was determined to be 525.44 pg/mL in mouse heart extract and 69.55 pg/mL in mouse liver extract.
- sELISA
Supplier Data
Sandwich ELISA - Mouse TFPI ELISA Kit (AB217776)
Example of mouse TFPI standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Mouse TFPI ELISA Kit (ab217776) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TFPI protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TFPI with 1.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mouse TFPI (tissue factor pathway inhibitor) is a secreted protein located on the endothelial surface, within platelets, in plasma, and on monocytes. Functionally, mouse TFPI is the primary inhibitor of intravascular tissue factor (TF) activity. TF is the principal protein that initiates blood coagulation in vivo. Thus, Mouse TFPI inhibits coagulation.
Mouse TFPI has an acidic N-terminal region, three Kunitz (K) domains separated by two linker regions, and a C-terminal basic region. The first K domain inhibits TF from binding coagulation factor VIIa. The second K domain inhibits factor Xa. The third K domain binds to heparin. Human and rat TFPI exhibit 58% and 71% amino acid identity to mouse TFPI, respectively.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Publications (1)
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International journal of molecular sciences 25: PubMed38256259
2024
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