AB277719

Mouse TGF beta Receptor II ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Mouse TGF beta Receptor II ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse TGF beta Receptor II with a sensitivity of 15.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

TGF-beta receptor type-2, TGFR-2, TGF-beta type II receptor, Transforming growth factor-beta receptor type II, TGF-beta receptor type II, TbetaR-II, Tgfbr2

4 Images

Sandwich ELISA - Mouse TGF beta Receptor II ELISA Kit (AB277719)

sELISA

Supplier Data

Sandwich ELISA - Mouse TGF beta Receptor II ELISA Kit (AB277719)

Example of mouse TGF beta Receptor II standard curve in Sample Diluent NS.

The TGF beta Receptor II standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse TGF beta Receptor II ELISA Kit (AB277719)

Interpolated concentrations of native TGF beta Receptor II in mouse serum, plasma and cell culture supernatant samples.

The concentrations of TGF beta Receptor II were measured in duplicates, interpolated from the TGF beta Receptor II standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 100%, plasma (EDTA) 100%, plasma (heparin) 50%, and lung supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TGF beta Receptor II concentration was determined to be 5.71 ng/mL in serum, 3.76 ng/mL in plasma (citrate), 4.36 ng/mL in plasma (EDTA), 6.30 ng/mL in plasma (heparin), and 2.14 ng/mL in and lung supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse TGF beta Receptor II ELISA Kit (AB277719)

Interpolated concentrations of native TGF beta Receptor II in mouse lung extract based on a 2,000 μg/mL extract load and mouse spleen extract based on a 500 μg/mL extract load.

The concentrations of TGF beta Receptor II were measured in duplicate and interpolated from the TGF beta Receptor II standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TGF beta Receptor II concentration was determined to be 2.94 ng/mL in lung extract and 1.34 ng/mL in spleen extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse TGF beta Receptor II ELISA Kit (AB277719)

Example of mouse TGF beta Receptor II standard curve in 1X Cell Extraction Buffer PTR.

The TGF beta Receptor II standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 15.2 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse TGF beta Receptor II ELISA kit (ab277719) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse TGF beta Receptor II protein in mouse serum, plasma, cell and tissue culture supernatant, and cell and tissue culture extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TGF beta Receptor II with 15.2 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Mouse TGF beta Receptor II is a transmembrane serine/threonine kinase that is formed with TGF-beta type I receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. TGF beta Receptor II transduces the TGFB1, TGFB2 and TGFB3 signal from surface of the cell to the cytoplasm. TGF beta Receptor II is involved in the regulation of cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Mouse TGF beta Receptor II shares 88.2%, 96.2%, 87.0%, and 91.7% sequence homology with human, rat, cow, and monkey, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 97 %", "average": "= 95" }, { "sample": "Serum", "range": "89 - 96 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "93 - 98 %", "average": "= 98" }, { "sample": "Tissue Extracts", "range": "81 - 86 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "92 - 94 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "90 - 95 %", "average": "= 93" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways (By similarity).. Isoform RII-1. Has transforming growth factor beta-activated receptor activity.. Isoform RII-2. Has transforming growth factor beta-activated receptor activity.

See full target information Tgfbr2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 30:59 PubMed38745316

2024

Polyphyllin I alleviates neuroinflammation after cerebral ischemia-reperfusion injury via facilitating autophagy-mediated M2 microglial polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyang Kang,Qiuling Sang,Dingxi Liu,Libo Wang,Jia Li,Xiaoyang Liu

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse TGF beta Receptor II ELISA Kit

Sandwich ELISA - Mouse TGF beta Receptor II ELISA Kit (AB277719)

Sandwich ELISA - Mouse TGF beta Receptor II ELISA Kit (AB277719)

Sandwich ELISA - Mouse TGF beta Receptor II ELISA Kit (AB277719)

Sandwich ELISA - Mouse TGF beta Receptor II ELISA Kit (AB277719)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

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