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AB137994

Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT)

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(29 Publications)

Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT) is a sandwich ELISA designed to quantify Mouse Thrombin-Antithrombin Complexes (TAT) with a sensitivity of 1.5 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 1.953 - 500 pg/mL
- Cited in over 20 publications
2 Images
ELISA - Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT) (AB137994)
  • ELISA

Lab

ELISA - Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT) (AB137994)

Mouse TAT measured in murine biological fluids (diluted 1/256; duplicates +/- SD).

Sandwich ELISA - Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT) (AB137994)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT) (AB137994)

Representative Standard Curve using ab137994.

Key facts

Detection method

Colorimetric

Sample types

Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

>= 1.5 pg/mL

Range

1.953 - 500 pg/mL

Assay time

5h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT) ab137994 is a sandwich ELISA to measure Mouse Thrombin-Antithrombin Complexes (TAT) in plasma with a sensitivity of 1.5 pg/ml.

How the assay works

A TAT Complexes specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TAT Complexes specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TAT Complexes captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT) ab137994 protocol summary

1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab137994-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB137994-CMP04", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB137994-CMP09", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB137994-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"TAT Complexes Standard", "number":"AB137994-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB137994-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TAT Complexes Microplate (12 x 8 well strips)", "number":"AB137994-CMP06", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB137994-CMP08", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Mouse TAT Complexes Antibody", "number":"AB137994-CMP01", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB137994-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Thrombin-Antithrombin Complex also referred to as TAT complex plays a significant mechanical role in regulating blood coagulation. It forms from the interaction of thrombin an enzyme and antithrombin a serpin inhibitor. Thrombin shows an approximate molecular mass of 36 kDa while antithrombin weighs around 58 kDa. This complex is expressed in the liver and circulates in the bloodstream where it helps maintain hemostasis by neutralizing excess thrombin preventing unwanted clotting events.
Biological function summary

The complex participates in the regulation of the coagulation cascade. It functions as an essential inhibitor forming part of a larger set of interactions that ensure blood coagulation occurs correctly. As a result it plays a role in maintaining the balance between coagulation and anticoagulation reducing the risk of thrombotic conditions. The TAT complex can be detected using specific kits like TAT kits which measure its levels important for understanding coagulation status in conditions using both human and mouse models.

Pathways

The thrombin-antithrombin complex is integrally involved in the intrinsic and extrinsic pathways of the coagulation cascade. It serves as a feedback mechanism to control and limit thrombin activity. The balance it provides is important to the effective regulation of fibrin formation working alongside proteins such as fibrinogen and factor Xa. Antithrombin in particular requires heparin or heparin-like molecules to enhance its inhibitory effects on thrombin ensuring precise modulation of coagulation.

Thrombin-antithrombin complex levels correlate with various thrombotic and hemorrhagic disorders. Elevated levels often indicate ongoing activation of the coagulation system seen in conditions like deep vein thrombosis and disseminated intravascular coagulation (DIC). These elevated levels can result from increased thrombin generation and antithrombin consumption. The complex's interaction with proteins like factor V and fibrin is pertinent in the pathology of these disorders making it a valuable marker in diagnosis and monitoring therapeutic responses.
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Product protocols

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Target data

See full target information Thrombin-Antithrombin Complex
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Publications (29)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed40094828

2025

Inhibiting NINJ1-dependent plasma membrane rupture protects against inflammasome-induced blood coagulation and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Cui,Hua Li,Dien Ye,Guoying Zhang,Yan Zhang,Ling Yang,Martha M S Sim,Jeremy P Wood,Yinan Wei,Zhenyu Li,Congqing Wu

Frontiers in immunology 16:1516120 PubMed40034692

2025

Caspase-11 and NLRP3 exacerbate systemic Klebsiella infection through reducing mitochondrial ROS production.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqi Zhou,Zhuodong Chai,Ankit Pandeya,Ling Yang,Yan Zhang,Guoying Zhang,Congqing Wu,Zhenyu Li,Yinan Wei

Hepatology communications 8: PubMed39101776

2024

Neutrophil extracellular traps induce intrahepatic thrombotic tendency and liver damage in cholestatic liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Muxin Yu,Xiaowen Li,Long Xu,Chuwei Zheng,Weiwei Pan,Hui Chen,Xiaoyu Liu,Xianshan Zhang,Jinming Zhang

International journal of molecular sciences 25: PubMed38256259

2024

Exploring the Hemostatic Effects of Platelet Lysate-Derived Vesicles: Insights from Mouse Models.

Applications

Unspecified application

Species

Unspecified reactive species

Nobuhisa Hirayu,Osamu Takasu

Frontiers in pharmacology 14:1304697 PubMed38143504

2023

Efficacies of S-nitrosoglutathione (GSNO) and GSNO reductase inhibitor in SARS-CoV-2 spike protein induced acute lung disease in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Judong Kim,Fei Qiao,Avtar K Singh,Jeseong Won,Inderjit Singh

Cell 186:4851-4867.e20 PubMed37848036

2023

Serotonin reduction in post-acute sequelae of viral infection.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea C Wong,Ashwarya S Devason,Iboro C Umana,Timothy O Cox,Lenka Dohnalová,Lev Litichevskiy,Jonathan Perla,Patrick Lundgren,Zienab Etwebi,Luke T Izzo,Jihee Kim,Monika Tetlak,Hélène C Descamps,Simone L Park,Stephen Wisser,Aaron D McKnight,Ryan D Pardy,Junwon Kim,Niklas Blank,Shaan Patel,Katharina Thum,Sydney Mason,Jean-Christophe Beltra,Michaël F Michieletto,Shin Foong Ngiow,Brittany M Miller,Megan J Liou,Bhoomi Madhu,Oxana Dmitrieva-Posocco,Alex S Huber,Peter Hewins,Christopher Petucci,Candice P Chu,Gwen Baraniecki-Zwil,Leila B Giron,Amy E Baxter,Allison R Greenplate,Charlotte Kearns,Kathleen Montone,Leslie A Litzky,Michael Feldman,Jorge Henao-Mejia,Boris Striepen,Holly Ramage,Kellie A Jurado,Kathryn E Wellen,Una O'Doherty,Mohamed Abdel-Mohsen,Alan L Landay,Ali Keshavarzian,Timothy J Henrich,Steven G Deeks,Michael J Peluso,Nuala J Meyer,E John Wherry,Benjamin A Abramoff,Sara Cherry,Christoph A Thaiss,Maayan Levy

Microbiological research 275:127460 PubMed37467711

2023

Inflammasome activation and pyroptosis mediate coagulopathy and inflammation in Salmonella systemic infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ankit Pandeya,Yan Zhang,Jian Cui,Ling Yang,Jeffery Li,Guoying Zhang,Congqing Wu,Zhenyu Li,Yinan Wei

TH open : companion journal to thrombosis and haemostasis 7:e1-e13 PubMed36751299

2023

Edoxaban, a Factor Xa-Specific Direct Oral Anticoagulant, Significantly Suppresses Tumor Growth in Colorectal Cancer Colon26-Inoculated BALB/c Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Keiichi Hiramoto,Nobuyuki Akita,Junji Nishioka,Koji Suzuki

EMBO molecular medicine 14:e15809 PubMed36345711

2022

Sex differences and risk factors for bleeding in Alagille syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Simona Hankeova,Noemi Van Hul,Jakub Laznovsky,Elisabeth Verboven,Katrin Mangold,Naomi Hensens,Csaba Adori,Elvira Verhoef,Tomas Zikmund,Feven Dawit,Michaela Kavkova,Jakub Salplachta,Marika Sjöqvist,Bengt R Johansson,Mohamed G Hassan,Linda Fredriksson,Karsten Baumgärtel,Vitezslav Bryja,Urban Lendahl,Andrew Jheon,Florian Alten,Kristina Teär Fahnehjelm,Björn Fischler,Jozef Kaiser,Emma R Andersson

Cell death & disease 13:722 PubMed35982051

2022

Novel 1-hydroxy phenothiazinium-based derivative protects against bacterial sepsis by inhibiting AAK1-mediated LPS internalization and caspase-11 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Yuan,Kelong Ai,Menghua Xiang,Chengliang Xie,Mingyi Zhao,Ming Wu,Hongli Li,Yueren Wu,Yueqing Cao,Can Li,Yanjun Zhong,Xiaomeng Pei,Helen Ka Wai Law,Liqian Gao,Qicai Xiao,Xinyu Yang
View all publications
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