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AB230933

Mouse Thrombin ELISA Kit

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(3 Publications)

Mouse Thrombin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Thrombin with a sensitivity of 17.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Cf2, F2, Prothrombin, Coagulation factor II

5 Images
ELISA - Mouse Thrombin ELISA Kit (AB230933)
  • ELISA

Supplier Data

ELISA - Mouse Thrombin ELISA Kit (AB230933)
Sandwich ELISA - Mouse Thrombin ELISA Kit (AB230933)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombin ELISA Kit (AB230933)

Example of mouse Thrombin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Thrombin ELISA Kit (AB230933)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombin ELISA Kit (AB230933)

Interpolated concentrations of native Thrombin in mouse serum and plasma.

The concentrations of Thrombin were measured in duplicate, interpolated from the Thrombin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 12000, plasma (citrate) 1 : 2000, plasma (EDTA) 1 : 500, and plasma (heparin) 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombin concentration was determined to be 37.1 μg/mL in serum, 3.7 μg/mL in plasma (citrate), 5.4 ug/mL in plasma (EDTA), and 4.48 μg/mL in plasma (heparin).

Sandwich ELISA - Mouse Thrombin ELISA Kit (AB230933)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombin ELISA Kit (AB230933)

Example of mouse Thrombin standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse Thrombin ELISA Kit (AB230933)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombin ELISA Kit (AB230933)

Interpolated concentrations of native Thrombin in mouse liver extract based on a 500 μg/mL extract load.

The concentrations of Thrombin were measured in duplicate and interpolated from the Thrombin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombin concentration was determined to be 2.29 ng/mL in mouse liver extract.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 17.5 pg/mL

Range

0.31 - 20 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Thrombin ELISA Kit ab230933 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Thrombin in citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 17.5 pg/mL.

How the assay works

Mouse Thrombin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Thrombin ELISA Kit ab230933 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Thrombin antibody [EPR20131-28] - BSA and Azide free (Capture) ab244973
- Anti-Thrombin antibody [EPR20131-271] - BSA and Azide free (Detector) ab244973

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
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Recovery

[ { "sample": "Serum", "range": "94 - 104 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "95 - 117 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "106 - 112 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "91 - 102 %", "average": "= 97" }, { "sample": "Cell culture media", "range": "92 - 103 %", "average": "= 99" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab230933-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB230933-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB230933-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB230933-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB230933-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB230933-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB230933-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Thrombin Lyophilized Recombinant Protein", "number":"AB230933-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB230933-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Thrombin Capture Antibody", "number":"AB230933-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB230933-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Thrombin Detector Antibody", "number":"AB230933-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB230933-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombin also known as Factor II or thrombin protein is a 36 kDa serine protease essential for blood coagulation. It is produced in the liver as prothrombin and activated in the coagulation cascade. Its expression occurs extensively in the liver where it plays an important role in converting soluble fibrinogen into insoluble fibrin forming blood clots. Thrombin also activates various other coagulation factors including Factors V VIII and XI amplifying the coagulation response. Biotinylated thrombin variations help in detection and research applications.
Biological function summary

Thrombin modulates several physiological processes beyond clot formation. It serves as a signaling molecule interacting with protease-activated receptors (PARs) to influence cell functions including proliferation migration and apoptosis. Thrombin forms part of the prothrombinase complex comprised of prothrombin activated Factor X (Xa) and Factor V on phospholipid surfaces. This complex is critical for thrombin generation during the clotting cascade. Thrombin's activity extends to involvement in wound healing and inflammation regulation.

Pathways

Thrombin significantly participates in the coagulation and fibrinolytic pathways. It not only converts fibrinogen to fibrin in the coagulation pathway but also activates inhibitors like antithrombin which regulates thrombin and other protease activities. Thrombin's interaction with fibrinolysis where tPA and plasminogen are substrates integrates clot disintegration processes. Thrombin's connectivity with proteins such as Factor VII Protein C and antithrombin outlines its diverse role in maintaining hemostatic balance.

Thrombin associates with conditions such as venous thromboembolism and disseminated intravascular coagulation (DIC). Dysregulation of thrombin leads to excessive clotting contributing to thromboembolic diseases. In DIC uncontrolled thrombin activity results in systemic coagulation consuming clotting factors and increasing bleeding risk. Its function alongside proteins like Factor V Leiden and antithrombin deficiencies aggravate thrombotic conditions. Research into thrombin inhibitors aids in developing therapeutic strategies for these disorders.
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Product protocols

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Target data

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity). Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces (By similarity). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells (By similarity).
See full target information F2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:5500 PubMed37679321

2023

Astrocyte reactivity and inflammation-induced depression-like behaviors are regulated by Orai1 calcium channels.

Applications

Unspecified application

Species

Unspecified reactive species

Michaela M Novakovic,Kirill S Korshunov,Rogan A Grant,Megan E Martin,Hiam A Valencia,G R Scott Budinger,Jelena Radulovic,Murali Prakriya

Theranostics 11:4251-4261 PubMed33754059

2021

Dabigatran activates inflammation resolution by promoting fibrinogen-like protein 2 shedding and RvD5 production.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Lei,Yu Zhou,Huakan Zhao,Yu Chen,Guifang Yan,Lei Wu,Yanquan Xu,Jiangang Zhang,Xiao Zhang,Jingchun Wang,Dingshan Li,Yongsheng Li

Cell death & disease 10:100 PubMed30718498

2019

β-arrestin-2 in PAR-1-biased signaling has a crucial role in endothelial function via PDGF-β in stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Hideaki Kanki,Tsutomu Sasaki,Shigenobu Matsumura,Satoru Yokawa,Toshiro Yukami,Munehisa Shimamura,Manabu Sakaguchi,Tadahide Furuno,Takahiro Suzuki,Hideki Mochizuki
View all publications
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websiteProtocolBooklet
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