AB209880

Mouse Thrombomodulin ELISA Kit

Recombinant
SimpleStep
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(4 Publications)

Mouse Thrombomodulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Thrombomodulin with a sensitivity of 17.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD141, Thrombomodulin, TM, Fetomodulin, Thbd

3 Images

Sandwich ELISA - Mouse Thrombomodulin ELISA Kit (AB209880)

sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombomodulin ELISA Kit (AB209880)

Interpolated concentrations of native Thrombomodulin in mouse urine and mouse lung supernatant samples and spiked Thrombomodulin in cell culture media.

The concentrations of Thrombomodulin were measured in duplicates, interpolated from the Thrombomodulin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 5%, mouse lung supernatant 1 : 150, and cell culture media 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombomodulin concentration was determined to be 81.9 ng/mL in urine, 805.5 ng/mL in lung supernatant and 25.8 ng/mL in cell culture media. Mouse lung supernatant was cultured for 6 days in cell culture media plus 10% Fetal Bovine Serum and 5 μg/mL LPS.

sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombomodulin ELISA Kit (AB209880)

Example of mouse Thrombomodulin standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombomodulin ELISA Kit (AB209880)

Interpolated concentrations of native Thrombomodulin in mouse serum, plasma (citrate), plasma (EDTA), and plasma (heparin).

The concentrations of Thrombomodulin were measured in duplicates, interpolated from the Thrombomodulin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 5%, plasma (EDTA) 5%, and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombomodulin concentration was determined to be 111.2 ng/mL in serum, 88.6 ng/mL in plasma (citrate), 95.4 ng/mL in plasma (EDTA), and 119.6 ng/mL in plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 17.5 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse Thrombomodulin ELISA Kit (ab209880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thrombomodulin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Thrombomodulin with 17.5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Mouse Thrombomodulin, which is encoded by the THBD gene, is an endothelial cell membrane protein that inhibits coagulation. Specifically, Thrombomodulin forms a 1:1 complex with thrombin, and this complex converts protein C to activated protein C. Activated protein C is an anticoagulant that inactivates coagulation factors Va and VIIIa. Human and rat Thrombomodulin show 67% and 86% amino acid sequence identity, respectively, to mouse Thrombomodulin. In humans, Thrombomodulin plays a role in thrombosis, arteriosclerosis, and cancer.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Mouse Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Intra", "sample": "Mouse Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]

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Recovery

[ { "sample": "Serum", "range": "101 - 107 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "97 - 103 %", "average": "= 101" }, { "sample": "Urine", "range": "98 - 115 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "86 - 99 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "93 - 115 %", "average": "= 102" }, { "sample": "Cell culture media", "range": "91 - 98 %", "average": "= 94" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab209880-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB209880-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB209880-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB209880-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB209880-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Thrombomodulin Detector Antibody", "number":"AB209880-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB209880-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB209880-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB209880-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Thrombomodulin Capture Antibody", "number":"AB209880-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Thrombomodulin Lyophilized Recombinant Protein", "number":"AB209880-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombomodulin also known as CD141 is a glycoprotein with a mass of approximately 74 kDa. It is an integral membrane protein expressed on the surface of endothelial cells. Thrombomodulin acts mechanically by binding thrombin an enzyme involved in blood coagulation to modulate its activity. This binding substantially alters thrombin's substrate specificity turning it into an anticoagulant enzyme that activates protein C instead of a procoagulant enzyme.

Biological function summary

Thrombomodulin regulates blood coagulation and maintains hemostatic balance. It forms a complex with thrombin on endothelial cells facilitating the conversion of protein C to activated protein C (APC). Activated protein C plays a pivotal role in controlling blood clot formation by proteolytically inactivating Factors Va and VIIIa. This function is important for preventing excessive clotting while ensuring proper wound healing processes.

Pathways

Thrombomodulin works chiefly within the anticoagulation pathway. It interacts directly with the thrombin-protein C pathway to achieve the activation of protein C. Additionally it interfaces with other pathways sharing mutual components such as endothelial cell proliferation and inflammation modulation. Related proteins include thrombin and protein C along with protein S which serves as a cofactor for APC's anticoagulant actions.

Thrombomodulin dysregulation can lead to thrombosis and vascular inflammation. A deficiency or altered expression of thrombomodulin has been linked to thromboembolic disorders and atherosclerosis. In these conditions improper activation of protein C in the presence of decreased thrombomodulin function can exacerbate clot formation. Monitoring the levels of proteins like thrombin and protein C within these diseases can provide insights into endothelial dysfunction and potential therapeutic approaches.

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Product protocols

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Target data

Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis. Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway. Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (By similarity). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (PubMed : 38174561). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (By similarity).

See full target information Thbd

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34830227

2021

Intramuscular Exposure to a Lethal Dose of Ricin Toxin Leads to Endothelial Glycocalyx Shedding and Microvascular Flow Abnormality in Mice and Swine.

Applications

Unspecified application

Species

Unspecified reactive species

Anita Sapoznikov,Yoav Gal,Yentl Evgy,Moshe Aftalion,Shahaf Katalan,Tamar Sabo,Chanoch Kronman,Reut Falach

Nature communications 11:6094 PubMed33323937

2020

A therapeutic combination of two small molecule toxin inhibitors provides broad preclinical efficacy against viper snakebite.

Applications

Unspecified application

Species

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Laura-Oana Albulescu,Chunfang Xie,Stuart Ainsworth,Jaffer Alsolaiss,Edouard Crittenden,Charlotte A Dawson,Rowan Softley,Keirah E Bartlett,Robert A Harrison,Jeroen Kool,Nicholas R Casewell

Medical science monitor : international medical jo 25:4811-4818 PubMed31253757

2019

Role of Programmed Cell Death 4 (PDCD4)-Mediated Akt Signaling Pathway in Vascular Endothelial Cell Injury Caused by Lower-Extremity Ischemia-Reperfusion in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Huanhuan Chen,Haifeng Zhu,Jin Yang,Yuefeng Zhu,Jinhua Mei,Haigang Shen,Kai Liang,Xiangyu Zhang

Chest 154:317-325 PubMed29555594

2018

Ultrastructural Alteration of Pulmonary Capillary Endothelial Glycocalyx During Endotoxemia.

Applications

Unspecified application

Species

Unspecified reactive species

Risa Inagawa,Hideshi Okada,Genzou Takemura,Kodai Suzuki,Chihiro Takada,Hirohisa Yano,Yoshiaki Ando,Takahiro Usui,Yasuaki Hotta,Nagisa Miyazaki,Akiko Tsujimoto,Ryogen Zaikokuji,Atsumu Matsumoto,Tomonori Kawaguchi,Tomoaki Doi,Takahiro Yoshida,Shozo Yoshida,Keisuke Kumada,Hiroaki Ushikoshi,Izumi Toyoda,Shinji Ogura

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Thrombomodulin ELISA Kit

Sandwich ELISA - Mouse Thrombomodulin ELISA Kit (AB209880)

Sandwich ELISA - Mouse Thrombomodulin ELISA Kit (AB209880)

Sandwich ELISA - Mouse Thrombomodulin ELISA Kit (AB209880)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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