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AB100748

Mouse Thrombopoietin ELISA Kit

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(2 Publications)

Mouse Thrombopoietin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Thrombopoietin in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Thrombopoietin, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ML, MGDF, Thpo

2 Images
Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB100748)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB100748)

Representative Standard Curve using ab100748

Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB100748)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB100748)

Representative Standard Curve using ab100748

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 30 pg/mL

Range

27.4 - 20000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Thrombopoietin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Thrombopoietin in serum, plasma and cell culture supernates.

This assay employs an antibody specific for Mouse Thrombopoietin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thrombopoietin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Thrombopoietin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thrombopoietin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 103 %", "average": "= 96.23" }, { "sample": "Serum", "range": "82 - 103 %", "average": "= 93.45" }, { "sample": "Plasma", "range": "84 - 104 %", "average": "= 94.28" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100748-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100748-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100748-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100748-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100748-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100748-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Thrombopoietin Microplate (12 x 8 wells)", "number":"AB100748-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse Thrombopoietin", "number":"AB100748-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100748-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse Thrombopoietin Standard (lyophilized)", "number":"AB100748-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombopoietin also known as TPO is an important glycoprotein hormone with a mass of about 70 kDa. It is primarily produced in the liver but can also be found in the kidneys and bone marrow stromal cells. Thrombopoietin binds to and activates its receptor c-Mpl which is present on hematopoietic progenitor cells. This protein has a significant role in regulating the production of blood platelets by stimulating the proliferation and differentiation of megakaryocytes the precursor cells for platelets.
Biological function summary

Thrombopoietin functions in hematopoiesis by supporting the maturation of megakaryocytes. It operates independently but can interact with other factors to optimize platelet production. Although it does not form a part of a large protein complex it is essential for the proper function of hematopoietic progenitors across several stages of differentiation. Its activity ensures the maintenance of platelet levels in the bloodstream therefore preventing conditions like thrombocytopenia.

Pathways

Thrombopoietin plays a critical role in the megakaryocyte/platelet lineage pathway. It directly influences the JAK-STAT (Janus kinase-signal transducer and activator of transcription) signaling pathway which is vital for driving the cell cycle and promoting cell survival. Thrombopoietin shares a functional connection with EPO (erythropoietin) which is involved in red blood cell production although they act on different lineage-specific progenitors.

Thrombopoietin is intricately linked to conditions like thrombocytopenia where there are abnormally low platelet counts and thrombocythemia characterized by elevated platelet levels. Dysregulation of Thrombopoietin production or its receptor activity can lead to these disorders. It has a relationship with other hematopoietic growth factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which can also influence the disorders of blood cell lines. Understanding its signaling pathways provides insights into therapeutic targets for diseases involving platelet imbalances.
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Product protocols

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Target data

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
See full target information Thpo
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 29:657-671 PubMed36090760

2022

Chemically modified -transcribed mRNA encoding thrombopoietin stimulates thrombopoiesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Xiaodong Xi,Hang Yu,Liuyan Yang,Jinzhong Lin,Wen Yang,Junling Liu,Xuemei Fan,Yingjie Xu

Cell death & disease 12:955 PubMed34657146

2021

Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Yan,Xinxin Ge,Ningbo Pang,Honglei Ye,Liuxia Yuan,Bin Cheng,Kangxi Zhou,Mengnan Yang,Yueyue Sun,Suqin Zhang,Zhongren Ding,Jincai Luo,Changgeng Ruan,Kesheng Dai
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