AB317546

Mouse Thrombopoietin ELISA Kit

SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Mouse Thrombopoietin ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Thrombopoietin with a sensitivity of 18.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Thrombopoietin, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ML, MGDF, Thpo

5 Images

Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB317546)

sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB317546)

Example of mouse Thrombopoietin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB317546)

Interpolated concentration of native Thrombopoietin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 25%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB317546)

Interpolated concentration of native Thrombopoietin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB317546)

Example of mouse Thrombopoietin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed

sELISA

Supplier Data

Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB317546)

Standard curve comparison between Mouse Thrombopoietin SimpleStep ELISA kit (ab317546) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cell culture media

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 18.8 pg/mL

Range

37.5 - 2400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse Thrombopoietin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thrombopoietin protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Mouse Thrombopoietin with 18.8 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.9" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "95 - 107 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "92 - 117 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "91 - 97 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "88 - 105 %", "average": "= 97" }, { "sample": "Cell culture media", "range": "98 - 105 %", "average": "= 102" } ]

style

left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab317546-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB317546-CMP08" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317546-CMP06" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB317546-CMP07" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317546-CMP05" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB317546-CMP04" }, { "size":"20 x 1 Vial", "name":"Mouse Thrombopoietin Lyophilized Recombinant Protein", "number":"AB317546-CMP03" }, { "size":"10 x 600 µL", "name":"10X Mouse Thrombopoietin Capture Antibody", "number":"AB317546-CMP01" }, { "size":"10 x 600 µL", "name":"10X Mouse Thrombopoietin Detector Antibody", "number":"AB317546-CMP02" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB317546-CMP10" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317546-CMP09" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab317546-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB317546-CMP08" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317546-CMP06" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB317546-CMP07" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB317546-CMP04" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317546-CMP05" }, { "size":"1 x 600 µL", "name":"10X Mouse Thrombopoietin Capture Antibody", "number":"AB317546-CMP01" }, { "size":"2 x 1 Vial", "name":"Mouse Thrombopoietin Lyophilized Recombinant Protein", "number":"AB317546-CMP03" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB317546-CMP11" }, { "size":"1 x 600 µL", "name":"10X Mouse Thrombopoietin Detector Antibody", "number":"AB317546-CMP02" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317546-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab317546-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB317546-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317546-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB317546-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317546-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Thrombopoietin Lyophilized Recombinant Protein", "number":"AB317546-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB317546-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Thrombopoietin Capture Antibody", "number":"AB317546-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Thrombopoietin Detector Antibody", "number":"AB317546-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317546-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317546-CMP10", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombopoietin also known as TPO is an important glycoprotein hormone with a mass of about 70 kDa. It is primarily produced in the liver but can also be found in the kidneys and bone marrow stromal cells. Thrombopoietin binds to and activates its receptor c-Mpl which is present on hematopoietic progenitor cells. This protein has a significant role in regulating the production of blood platelets by stimulating the proliferation and differentiation of megakaryocytes the precursor cells for platelets.

Biological function summary

Thrombopoietin functions in hematopoiesis by supporting the maturation of megakaryocytes. It operates independently but can interact with other factors to optimize platelet production. Although it does not form a part of a large protein complex it is essential for the proper function of hematopoietic progenitors across several stages of differentiation. Its activity ensures the maintenance of platelet levels in the bloodstream therefore preventing conditions like thrombocytopenia.

Pathways

Thrombopoietin plays a critical role in the megakaryocyte/platelet lineage pathway. It directly influences the JAK-STAT (Janus kinase-signal transducer and activator of transcription) signaling pathway which is vital for driving the cell cycle and promoting cell survival. Thrombopoietin shares a functional connection with EPO (erythropoietin) which is involved in red blood cell production although they act on different lineage-specific progenitors.

Thrombopoietin is intricately linked to conditions like thrombocytopenia where there are abnormally low platelet counts and thrombocythemia characterized by elevated platelet levels. Dysregulation of Thrombopoietin production or its receptor activity can lead to these disorders. It has a relationship with other hematopoietic growth factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which can also influence the disorders of blood cell lines. Understanding its signaling pathways provides insights into therapeutic targets for diseases involving platelet imbalances.

style

left-column

Product protocols

style

left-column

Target data

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

See full target information Thpo

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Thrombopoietin ELISA Kit

Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB317546)

Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB317546)

Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB317546)

Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB317546)

Sandwich ELISA - Mouse Thrombopoietin ELISA Kit (AB317546)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Assay Kits

Primary Antibodies

Assay Kits

Product promise