Mouse Thrombospondin 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Thrombospondin 1 with a sensitivity of 0.054 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.203 - 13 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.054 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions (By similarity). Multifunctional, involved in inflammation, angiogenesis, wound healing, reactive oxygen species (ROS) signaling, nitrous oxide (NO) signaling, apoptosis, senescence, aging, cellular self-renewal, stemness, and cardiovascular and metabolic homeostasis (By similarity). Negatively modulates dendritic cell activation and cytokine release, as part of an autocrine feedback loop, contributing to the resolution of inflammation and immune homeostasis (By similarity). Ligand for receptor CD47 (By similarity). Modulates nitrous oxide (NO) signaling via CD47, hence playing a role as a pressor agent, supporting blood pressure (PubMed:20610415). Plays a role in endothelial cell senescence, acting via CD47, by increasing the abundance and activation of NADPH oxidase NOX1, and so generating excess ROS (PubMed:29042481). Inhibits stem cell self-renewal, acting via CD47 signaling, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (PubMed:23591719). Negatively modulates wound healing, acting via CD47 (PubMed:18156939). Ligand for receptor CD36 (By similarity). Involved in inducing apoptosis in podocytes in response to elevated free fatty acids, acting via CD36 (PubMed:25835637). Plays a role in suppressing angiogenesis, acting, depending on context, via CD36 or CD47 (By similarity). Promotes cellular senescence in a TP53-CDKN1A-RB1 signaling-dependent manner (PubMed:29042481). Ligand for immunoglobulin-like cell surface receptor SIRPA (By similarity). Involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production, acting via interaction with SIRPA (By similarity). Plays a role in metabolic dysfunction in diet-induced obesity, perhaps acting by exacerbating adipose inflammatory activity; its effects may be mediated, at least in part, through enhanced adipocyte proliferation (PubMed:23757408). Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (PubMed:22682248). May be involved in age-related conditions, including metabolic dysregulation, during normal aging (PubMed:29042481, PubMed:32679764).
Alternative names
Tsp1, Thbs1, Thrombospondin-1, Glycoprotein G
What's included?
Recommended products
Mouse Thrombospondin 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Thrombospondin 1 with a sensitivity of 0.054 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 0.203 - 13 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.054 ng/mL
- General recovery
- 98%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 8 | mean - | SD - | C.V. 5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Plasma | n 3 | mean - | SD - | C.V. 7.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 98 | Range 95 - 100 % |
Sample type EDTA Plasma | Average % = 110 | Range 104 - 120 % |
Sample type Citrate plasma | Average % = 99 | Range 89 - 106 % |
Sample type Heparin Plasma | Average % = 100 | Range 97 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse Thrombospondin 1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thrombospondin 1 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma. Quantitate Mouse Thrombospondin 1 with 0.054 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thrombospondin 1 also known as TSP1 is a multi-functional glycoprotein involved in cellular processes like adhesion migration and tumorigenesis. It has a molecular weight of approximately 450 kDa. TSP1 is produced by multiple cell types including endothelial cells and fibroblasts and it is widely expressed in the extracellular matrix of both normal and tumor tissues. As a member of the thrombospondin family it interacts with cell receptors like integrins and CD36 to mediate its diverse functions.
- Biological function summary
TSP1 is significant in modulating angiogenesis immune response and apoptosis. It acts as an anti-angiogenic factor by inhibiting endothelial cell proliferation and migration. TSP1 is a part of a larger complex where it interacts with other matrix proteins and growth factors to fine-tune cellular behavior within tissues. Through its binding domains it inhibits matrix metalloproteinases helping regulate matrix composition and tissue remodeling.
- Pathways
TSP1 influences both the TGF-beta and NF-kB signaling pathways. In the TGF-beta pathway TSP1 activates latent TGF-beta contributing to tissue repair and fibrosis processes. It works synergistically with proteins like SMADs in these pathways. In the NF-kB pathway TSP1 modulates immune responses and inflammatory processes. Its interaction with CD36 and integrins in these pathways underlines its versatile role in cellular signaling.
- Associated diseases and disorders
TSP1 has been investigated in cancer progression and cardiovascular diseases. In cancer TSP1's anti-angiogenic properties suppress tumor vascularization which can slow tumor growth. Aberrant TSP1 expression levels have also been linked to atherosclerosis development as it interacts with endothelial cells and inflammatory molecules like TNF-alpha. In both these contexts TSP1 engages with proteins such as integrins and CD36 to exert its influence on disease progression.
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4 product images
Sandwich ELISA - Mouse Thrombospondin 1 ELISA Kit (ab317551)
Example of mouse Thrombospondin 1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Thrombospondin 1 ELISA Kit (ab317551)
Interpolated concentration of native Thrombospondin 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 1:2,000, plasma (citrate) 1:100, plasma (EDTA) 1:200, and plasma (heparin) 1:500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse Thrombospondin 1 ELISA Kit (ab317551)
Example of mouse Thrombospondin 1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse Thrombospondin 1 ELISA Kit (ab317551)
Interpolated concentration of native Thrombospondin 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:200 plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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