Mouse TIMP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse TIMP1 with a sensitivity of 6.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Timp, Timp-1, Timp1, Metalloproteinase inhibitor 1, Collagenase inhibitor 16C8 fibroblast, Erythroid-potentiating activity, TPA-S1, TPA-induced protein, Tissue inhibitor of metalloproteinases 1, EPA, TIMP-1
- ELISA
Supplier Data
ELISA - Mouse TIMP1 ELISA Kit (AB196265)
- sELISA
Supplier Data
Sandwich ELISA - Mouse TIMP1 ELISA Kit (AB196265)
Interpolated concentrations of TIMP1 in Mouse serum and plasma (citrate).
The concentrations of TIMP1 were measured in duplicate and interpolated from the TIMP1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP1 concentration was determined to be 4,495 pg/mL in Mouse serum and 4,210 pg/mL in Mouse plasma.
- sELISA
Supplier Data
Sandwich ELISA - Mouse TIMP1 ELISA Kit (AB196265)
Comparison of secreted TIMP1 in unstimulated and PMA/PHA-stimulated L929 Cells.
L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. TIMP1 was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the TIMP1 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed : 4.1 ng/mL unstimulated, 18.5 ng/mL stimulated. There was no detectable signal in media.
- sELISA
Supplier Data
Sandwich ELISA - Mouse TIMP1 ELISA Kit (AB196265)
Example of TIMP1 standard curve prepared in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse TIMP1 ELISA Kit (AB196265)
Titration of L929 Extract diluted 62.5 µg/mL to 4 µg/mL in Sample Diluent NS.
Background subtracted data values (mean +/- SD, n = 2) are graphed.
- sELISA
Lab
Sandwich ELISA - Mouse TIMP1 ELISA Kit (AB196265)
Example of mouse TIMP1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Mouse TIMP1 ELISA Kit (AB196265)
Interpolated concentration of native TIMP1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 10% mouse serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Reactivity data
Product details
Mouse TIMP1 ELISA Kit ab196265 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse TIMP1 in cell culture extracts, cell culture supernatant, plasma, serum. This SimpleStep sensitivity is 6.2 pg/mL.
How the assay works
Mouse TIMP1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse TIMP1 ELISA Kit ab196265 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-TIMP1 antibody [EPR16616-207] - BSA and Azide free (Capture) ab242911
- Anti-TIMP1 antibody [EPR16616-204] - BSA and Azide free (Detector) ab242911
Tissue metalloproteinase inhibitor 1 (TIMP1or Metalloproteinase inhibitor 1) inhibits matrix metalloproteinases (MMP's) which cause degradation of the extracellular matrix. TIMP1 inhibits MMP's by forming a non-covalent bond complex at their catalytic zinc cofactor site and irreversibly inactivating them. TIMP1 also promotes cell proliferation and has some anti-apoptotic functions.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.
Pathways
TIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (3)
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Frontiers in pharmacology 14:1176073 PubMed37351505
2023
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Turkish journal of surgery 38:255-265 PubMed36846057
2022
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Molecular psychiatry 23:2302-2313 PubMed30254235
2018
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Product promise
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