Mouse TIMP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse TIMP1 with a sensitivity of 6.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Frontiers in pharmacology 14:11760732023Protective effect of Briq. ethanolic extract against UVB-induced skin aging and photodamage in hairless mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Ji Gu,Hee-Weon Lee,Guijae Yoo,Donghwan Kim,In-Wook Choi,Yoonsook Kim,Sang Keun HaPubMed 37351505
- Turkish journal of surgery 38:255-2652022Anti-inflammatory effects of oral and intraperitoneal administration of cerium oxide nanoparticles on experimental hepatic ischemia-reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkile Zengin,Açelya Erikçi,Gökçen Telli,Bülent Gümüşel,Kemal Kösemehmetoğlu,Gülberk Uçar,Mustafa Cem AlgınPubMed 36846057
- Molecular psychiatry 23:2302-23132018The hippocampal extracellular matrix regulates pain and memory after injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaral Tajerian et. al.PubMed 30254235
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 15.6 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 6.2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14 (By similarity). Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.
Alternative names
Timp, Timp-1, Timp1, Metalloproteinase inhibitor 1, Collagenase inhibitor 16C8 fibroblast, Erythroid-potentiating activity, TPA-S1, TPA-induced protein, Tissue inhibitor of metalloproteinases 1, EPA, TIMP-1
What's included?
Recommended products
Mouse TIMP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse TIMP1 with a sensitivity of 6.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 15.6 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 6.2 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 6.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 111 | Range 110 - 113 % |
Sample type Cell culture extracts | Average % = 104 | Range 90 - 119 % |
Sample type Cell culture media | Average % = 107 | Range 106 - 108 % |
Sample type Citrate plasma | Average % = 112 | Range 109 - 113 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mouse TIMP1 ELISA Kit (ab196265) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TIMP1 protein in cell culture supernatant, serum, plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TIMP1 with 6.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Tissue metalloproteinase inhibitor 1 (TIMP1or Metalloproteinase inhibitor 1) inhibits matrix metalloproteinases (MMP's) which cause degradation of the extracellular matrix. TIMP1 inhibits MMP's by forming a non-covalent bond complex at their catalytic zinc cofactor site and irreversibly inactivating them. TIMP1 also promotes cell proliferation and has some anti-apoptotic functions.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tissue Inhibitor of Metalloproteinase 1 (TIMP1) is a protein with a molecular mass of approximately 28 kDa. The protein inhibits metalloproteinases by binding to them preventing the breakdown of the extracellular matrix. This action regulates extracellular environment and tissue remodeling. TIMP1 is also known by the name Erythroid-Potentiating Activity (EPA). It is expressed in various tissues including the liver and the brain and can be found in high levels in the blood serum.
- Biological function summary
TIMP1 plays several essential roles beyond inhibiting metalloproteinases. It supports cell proliferation and influences apoptosis. It is part of a larger TIMP family and does not function as part of a complex per se but interacts closely with matrix metalloproteinases (MMPs). By regulating MMP activity TIMP1 balances processes like tissue growth and inflammatory responses which are important for normal development and repair.
- Pathways
TIMP1 participates in the regulation of the matrix metalloproteinase pathway impacting tissue homeostasis and repair. It closely associates with proteins such as MMP-9 and MMP-2 within this pathway. TIMP1 also plays a role in cytokine signaling pathways which influence immune responses by interacting with other signaling molecules and receptors that govern these pathways.
- Associated diseases and disorders
TIMP1's regulation of MMPs links it to cancer and cardiovascular disease progression. Overexpression of TIMP1 associates with tumor growth and metastasis where it can modulate interactions with other proteins like MMP-9 leading to altered tissue invasion and angiogenesis. Additionally TIMP1 imbalance connects to tissue fibrosis in cardiovascular disorders working in tandem with proteins such as MMP-2 which affects the structural remodeling and function of tissues within the cardiovascular system.
Product promise
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6 product images
Sandwich ELISA - Mouse TIMP1 ELISA Kit (ab196265)
Example of TIMP1 standard curve prepared in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Mouse TIMP1 ELISA Kit (ab196265)
Interpolated concentrations of TIMP1 in Mouse serum and plasma (citrate).
The concentrations of TIMP1 were measured in duplicate and interpolated from the TIMP1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP1 concentration was determined to be 4,495 pg/mL in Mouse serum and 4,210 pg/mL in Mouse plasma.
Sandwich ELISA - Mouse TIMP1 ELISA Kit (ab196265)
Titration of L929 Extract diluted 62.5 µg/mL to 4 µg/mL in Sample Diluent NS.
Background subtracted data values (mean +/- SD, n = 2) are graphed.
Sandwich ELISA - Mouse TIMP1 ELISA Kit (ab196265)
Comparison of secreted TIMP1 in unstimulated and PMA/PHA-stimulated L929 Cells.
L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. TIMP1 was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the TIMP1 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 4.1 ng/mL unstimulated, 18.5 ng/mL stimulated. There was no detectable signal in media.
Sandwich ELISA - Mouse TIMP1 ELISA Kit (ab196265)
Interpolated concentration of native TIMP1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 10% mouse serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Mouse TIMP1 ELISA Kit (ab196265)
Example of mouse TIMP1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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