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AB100746

Mouse TIMP2 ELISA Kit

5

(2 Reviews)

|

(3 Publications)

Mouse TIMP2 ELISA Kit is a Sandwich ELISA for the measurement of Mouse TIMP2 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Timp-2, Timp2, Metalloproteinase inhibitor 2, Tissue inhibitor of metalloproteinases 2, TIMP-2

2 Images
Sandwich ELISA - Mouse TIMP2 ELISA Kit (AB100746)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TIMP2 ELISA Kit (AB100746)

Representative Standard Curve using ab100746

Sandwich ELISA - Mouse TIMP2 ELISA Kit (AB100746)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TIMP2 ELISA Kit (AB100746)

Representative Standard Curve using ab100746

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 80 pg/mL

Range

0.0823 - 60 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TIMP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TIMP2 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Mouse TIMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TIMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "82 - 105 %", "average": "= 95.36" }, { "sample": "Serum", "range": "80 - 112 %", "average": "= 92.65" }, { "sample": "Plasma", "range": "81 - 103 %", "average": "= 93.42" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100746-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100746-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100746-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100746-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TIMP2 Microplate (12 x 8 wells)", "number":"AB100746-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100746-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100746-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse TIMP2", "number":"AB100746-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse TIMP2 Standard (lyophilized)", "number":"AB100746-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"260X HRP-Streptavidin Concentrate", "number":"AB100746-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIMP2 also known as Tissue Inhibitor of Metalloproteinases 2 mechanistically inhibits the activity of matrix metalloproteinases (MMPs). It binds to MMPs regulating the extracellular matrix's remodeling. The protein has a molecular mass of around 21 kDa. TIMP2 is expressed in various tissues including the brain liver and kidneys where it maintains a balance between matrix degradation and production.
Biological function summary

TIMP2 plays a significant role in tissue maintenance and repair by controlling extracellular matrix turnover. It forms complexes with MMPs such as MMP-2 inhibiting their proteolytic activities. The protein is also involved in cell proliferation and differentiation affecting processes like angiogenesis and wound healing. Its regulatory function aids in maintaining tissue homeostasis and preventing excessive matrix breakdown.

Pathways

TIMP2 is integral to the matrix metalloproteinase pathway and the regulation of endothelial cell migration. The protein interacts closely with other MMPs influencing their activation and modulation. Through these pathways TIMP2 holds an important position in cellular migration and signaling ensuring precise matrix remodeling in response to physiological needs.

TIMP2 has significant links with cancer and fibrosis. Dysregulation of TIMP2 expression or its imbalance with MMPs can contribute to tumor progression and metastasis due to uncontrolled matrix degradation. Additionally abnormal TIMP2 levels associate with fibrotic diseases where excessive tissue deposition occurs. TIMP2's interaction with proteins like MMP-9 and MMP-2 highlights its involvement in these pathological conditions.
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Product protocols

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Target data

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
See full target information Timp2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 42:e111139 PubMed36382711

2022

Fecal microbiota transplantation enhances cell therapy in a rat model of hypoganglionosis by SCFA-induced MEK1/2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Donghao Tian,Wenyao Xu,Weikang Pan,Baijun Zheng,Weili Yang,Wanying Jia,Yong Liu,Malgorzata A Garstka,Ya Gao,Hui Yu

International journal of molecular sciences 21: PubMed32481745

2020

Cdc42-Dependent Transfer of mir301 from Breast Cancer-Derived Extracellular Vesicles Regulates the Matrix Modulating Ability of Astrocytes at the Blood-Brain Barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Golnaz Morad,Cassandra C Daisy,Hasan H Otu,Towia A Libermann,Simon T Dillon,Marsha A Moses

Molecular psychiatry 23:2302-2313 PubMed30254235

2018

The hippocampal extracellular matrix regulates pain and memory after injury.

Applications

Unspecified application

Species

Unspecified reactive species

Maral Tajerian,Victor Hung,Huy Nguyen,Gail Lee,Lydia-Marie Joubert,Andrey Victorovich Malkovskiy,Bende Zou,Simon Xie,Ting-Ting Huang,J David Clark
View all publications
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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