AB214091

Mouse Tissue Factor ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(9 Publications)

Mouse Tissue Factor ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Tissue Factor with a sensitivity of 3.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

CD142, Cf-3, Cf3, F3, Tissue factor, TF, Coagulation factor III

4 Images

Sandwich ELISA - Mouse Tissue Factor ELISA Kit (AB214091)

sELISA

Supplier Data

Sandwich ELISA - Mouse Tissue Factor ELISA Kit (AB214091)

Example of mouse Tissue Factor standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Tissue Factor ELISA Kit (AB214091)

Interpolated concentrations of native Tissue Factor in mouse brain cell culture supernatant and mouse urine samples.

The concentrations of Tissue Factor were measured in duplicates, interpolated from the Tissue Factor standard curves and corrected for sample dilution. Undiluted samples are as follows : mouse brain cell culture supernatant 100%, mouse urine 100%. For mouse urine, dilutions beyond 2 fold were not analysed due to O.D. values below the O.D. values of the MDD. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 79 pg/mL in mouse brain cell culture supernatant and 11 pg/mL in mouse urine.

sELISA

Supplier Data

Sandwich ELISA - Mouse Tissue Factor ELISA Kit (AB214091)

Interpolated concentrations of native Tissue Factor in mouse cell and tissue extract samples based on a 75 μg/mL extract load.

The concentrations of Tissue Factor were measured in duplicate and interpolated from the Tissue Factor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 713 pg/mL in Mouse Brain Tissue Extract, 206 pg/mL in mouse C2C12 Cell Extract and 824 pg/mL in NIH/3T3 Cell Extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse Tissue Factor ELISA Kit (AB214091)

Example of mouse Tissue Factor standard curve Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture extracts, Tissue Extracts, Cell culture supernatant

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 3.7 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Mouse Tissue Factor ELISA Kit (ab214091) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Tissue Factor protein in cell culture extracts, cell culture supernatant, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Tissue Factor with 3.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Tissue factor plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Tissue Factor initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The tissue factor-VIIa complex activates factors IX or X by specific limited protolysis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "87 - 98 %", "average": "= 92" }, { "sample": "Cell culture extracts", "range": "117 - 122 %", "average": "= 119" }, { "sample": "Urine", "range": "97 - 99 %", "average": "= 99" }, { "sample": "Mouse brain extract", "range": "107 - 110 %", "average": "= 109" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab214091-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB214091-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB214091-CMP13", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse Tissue Factor Capture Antibody (Lyophilized)", "number":"AB214091-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB214091-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Tissue Factor Lyophilized Recombinant Protein", "number":"AB214091-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB214091-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB214091-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Tissue Factor Detector Antibody", "number":"AB214091-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB214091-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB214091-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB214091-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB214091-CMP04", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Factor also known as coagulation factor III or CD142 is an important player in the blood coagulation process. This protein with a molecular mass of approximately 47 kDa is a transmembrane glycoprotein. It is expressed widely in various tissues including the lungs heart and brain and plays an important role in initiating the coagulation cascade when exposed to blood. In endothelial cells tissue factor is generally not present unless these cells are in a state of disturbance supporting its role as a primary defense mechanism against vascular injury.

Biological function summary

Tissue factor acts as a cofactor for factor VIIa forming a complex that rapidly catalyzes the activation of factor X to factor Xa. This catalytic action ultimately leads to fibrin formation which is essential for clotting. Tissue Factor (TF) is part of the extrinsic pathway of the coagulation cascade and is fundamental in the generation of a hemostatic response to tissue injury. By regulating thrombin generation through its interaction with other coagulation factors tissue factor plays an important role in maintaining hemostatic balance.

Pathways

Tissue factor is integral in the extrinsic pathway of coagulation which works closely with the intrinsic pathway to ensure effective hemostasis. It complexes with factor VIIa and triggers downstream effects involving proteins like factor X and thrombin. Tissue factor forms a bridge between cellular injury responses and the blood coagulation system linking these two significant physiological processes. The interaction with these proteins highlights its central role in hemostatic pathways and highlights its significance in the coagulation network.

Tissue factor is associated with conditions like thrombosis and cardiovascular diseases where its expression can become dysregulated. Abnormal activation of tissue factor contributes directly to the pathological clot formation seen in thrombosis. It is also implicated in cancer-related coagulopathy where it can facilitate tumor-associated thrombosis by interacting with cancer cell surfaces. Additionally in such diseases the interplay between tissue factor and thrombin becomes important as it affects disease progression and the severity of clot-related complications.

style

left-column

Product protocols

style

left-column

Target data

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF : VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

See full target information F3

style

left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Research and practice in thrombosis and haemostasis 8:102325 PubMed38404939

2024

Evaluation of the ability of commercial enzyme-linked immunosorbent assays to measure mouse tissue factor.

Applications

Unspecified application

Species

Unspecified reactive species

Ana T A Sachetto,Nigel Mackman

Cell 186:4851-4867.e20 PubMed37848036

2023

Serotonin reduction in post-acute sequelae of viral infection.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea C Wong,Ashwarya S Devason,Iboro C Umana,Timothy O Cox,Lenka Dohnalová,Lev Litichevskiy,Jonathan Perla,Patrick Lundgren,Zienab Etwebi,Luke T Izzo,Jihee Kim,Monika Tetlak,Hélène C Descamps,Simone L Park,Stephen Wisser,Aaron D McKnight,Ryan D Pardy,Junwon Kim,Niklas Blank,Shaan Patel,Katharina Thum,Sydney Mason,Jean-Christophe Beltra,Michaël F Michieletto,Shin Foong Ngiow,Brittany M Miller,Megan J Liou,Bhoomi Madhu,Oxana Dmitrieva-Posocco,Alex S Huber,Peter Hewins,Christopher Petucci,Candice P Chu,Gwen Baraniecki-Zwil,Leila B Giron,Amy E Baxter,Allison R Greenplate,Charlotte Kearns,Kathleen Montone,Leslie A Litzky,Michael Feldman,Jorge Henao-Mejia,Boris Striepen,Holly Ramage,Kellie A Jurado,Kathryn E Wellen,Una O'Doherty,Mohamed Abdel-Mohsen,Alan L Landay,Ali Keshavarzian,Timothy J Henrich,Steven G Deeks,Michael J Peluso,Nuala J Meyer,E John Wherry,Benjamin A Abramoff,Sara Cherry,Christoph A Thaiss,Maayan Levy

Thrombosis and haemostasis 123:1017-1033 PubMed37168007

2023

Monocyte Tissue Factor Expression: Lipopolysaccharide Induction and Roles in Pathological Activation of Coagulation.

Applications

Unspecified application

Species

Unspecified reactive species

Ana T A Sachetto,Nigel Mackman

Stem cell research & therapy 12:72 PubMed33468250

2021

MSC-NTF (NurOwn®) exosomes: a novel therapeutic modality in the mouse LPS-induced ARDS model.

Applications

Unspecified application

Species

Unspecified reactive species

Haggai Kaspi,Jonathan Semo,Nathalie Abramov,Chen Dekel,Stacy Lindborg,Ralph Kern,Chaim Lebovits,Revital Aricha

Cell communication and signaling : CCS 18:104 PubMed32641132

2020

Phospho-Tyr705 of STAT3 is a therapeutic target for sepsis through regulating inflammation and coagulation.

Applications

Unspecified application

Species

Unspecified reactive species

Shunyao Xu,Xiaojun Pan,Lingjie Mao,Hao Pan,Wenwei Xu,Yufeng Hu,Xueshu Yu,Zhiqiang Chen,Songzan Qian,Yincai Ye,Yueyue Huang,Jingye Pan

TH open : companion journal to thrombosis and haem 4:e66-e76 PubMed32309772

2020

Assessment of Thrombotic and Bleeding Tendency in Two Mouse Models of Chronic Kidney Disease: Adenine-Diet and 5/6th Nephrectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Camélia Makhloufi,Lydie Crescence,Roxane Darbousset,Nathalie McKay,Ziad A Massy,Christophe Dubois,Laurence Panicot-Dubois,Stéphane Burtey,Stéphane Poitevin

Cell host & microbe 27:556-570.e6 PubMed32142632

2020

TMEM173 Drives Lethal Coagulation in Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Ling Zeng,Min Xie,Jiao Liu,Borong Zhou,Runliu Wu,Lizhi Cao,Guido Kroemer,Haichao Wang,Timothy R Billiar,Herbert J Zeh,Rui Kang,Jianxin Jiang,Yan Yu,Daolin Tang

Thrombosis and haemostasis 118:1470-1480 PubMed30060257

2018

Elevating Endogenous Sphingosine-1-Phosphate (S1P) Levels Improves Endothelial Function and Ameliorates Atherosclerosis in Low Density Lipoprotein Receptor-Deficient (LDL-R-/-) Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Renata Feuerborn,Manuela Besser,Francesco Potì,Ralph Burkhardt,Gabriele Weißen-Plenz,Uta Ceglarek,Manuela Simoni,Richard L Proia,Hendrik Freise,Jerzy-Roch Nofer

International journal of molecular medicine 40:1869-1880 PubMed29039441

2017

Rivaroxaban attenuates thrombosis by targeting the NF-κB signaling pathway in a rat model of deep venous thrombus.

Applications

Unspecified application

Species

Unspecified reactive species

Junhao Ma,Xinxi Li,Yang Wang,Zhenwei Yang,Jun Luo

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse Tissue Factor ELISA Kit

Sandwich ELISA - Mouse Tissue Factor ELISA Kit (AB214091)

Sandwich ELISA - Mouse Tissue Factor ELISA Kit (AB214091)

Sandwich ELISA - Mouse Tissue Factor ELISA Kit (AB214091)

Sandwich ELISA - Mouse Tissue Factor ELISA Kit (AB214091)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Complementary Products

Assay Kits

Secondary Antibodies

Assay Kits

Assay Kits

Assay Kits

Product promise