AB224880

Mouse TLR2 ELISA Kit

Recombinant
SimpleStep
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(4 Publications)

Mouse TLR2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse TLR2 with a sensitivity of 88 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD282, Toll-like receptor 2, Tlr2

5 Images

Sandwich ELISA - Mouse TLR2 ELISA Kit (AB224880)

sELISA

Supplier Data

Sandwich ELISA - Mouse TLR2 ELISA Kit (AB224880)

Example of Mouse TLR2 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse TLR2 ELISA Kit (AB224880)

Interpolated concentrations of spike TLR2 in cell culture media sample.

The concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted sample is 50% cell culture media. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse TLR2 ELISA Kit (AB224880)

Example of Mouse TLR2 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse TLR2 ELISA Kit (AB224880)

Interpolated concentrations of native TLR2 in Mouse cell and tissue extract samples.

The concentrations of TLR2 were measured in duplicate and interpolated from the TLR2 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TLR2 concentration was determined to be 11.1 pg/μg in J774A.1 Cell Extract and 1.75 pg/μg in Spleen Tissue Extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse TLR2 ELISA Kit (AB224880)

Interpolated concentrations of spike TLR2 in Mouse serum and plasma samples.

The concentrations of TLR2 were measured in duplicates, interpolated from the TLR2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 88 pg/mL

Range

312.5 - 20000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mouse TLR2 ELISA Kit (ab224880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TLR2 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TLR2 with 88 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Toll-Like Receptor 2 (TLR2) is a transmembrane receptor, expressed on a wide variety of cell types including microglia, monocytes, macrophages, and dendritic cells. TLR2 cooperates with multiple other proteins (TLR1, LY96, TLR6, and other) to mediate an immune response to bacterial lipoproteins. Cytokine secretion in response to these bacterium is found to occur in a TLR2 dependent fashion. ab224880 recognizes the extracellular domain of the transmembrane receptor.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.1" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]

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Recovery

[ { "sample": "Serum", "range": "89 - 98 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "91 - 103 %", "average": "= 97" }, { "sample": "Cell culture extracts", "range": "91 - 112 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "88 - 91 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "96 - 103 %", "average": "= 99" }, { "sample": "Cell culture media", "range": "98 - 102 %", "average": "= 101" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab224880-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB224880-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB224880-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB224880-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB224880-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB224880-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB224880-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse TLR2 Capture Antibody", "number":"AB224880-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB224880-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse TLR2 Lyophilized Recombinant Protein", "number":"AB224880-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB224880-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB224880-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse TLR2 Detector Antibody", "number":"AB224880-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Toll-like receptor 2 (TLR2) also called CD282 is a protein that detects molecules from bacteria and fungi. It has a mass of about 90 kDa. TLR2 is expressed on the surface of immune cells such as monocytes macrophages and neutrophils. This receptor recognizes pathogen-associated molecular patterns (PAMPs) and activates the immune response.

Biological function summary

TLR2 initiates signaling pathways that lead to the production of cytokines and chemokines helping to control infection. It forms dimers with other TLRs like TLR1 or TLR6 to expand its recognition range of microbial components. TLR2's activity is important for balancing pathogen recognition and avoiding overactive immune response. On macrophages TLR2 activation enhances their phagocytic ability.

Pathways

TLR2 plays roles in innate immune signaling pathways and inflammation pathways. It contributes significantly to the NF-kB and MAPK pathways which regulate inflammation and immune response. TLR2 often cooperates with proteins like MyD88 and TRAF6 in these pathways leading to downstream activation of transcription factors and production of pro-inflammatory molecules.

TLR2 is associated with inflammatory and infectious diseases including sepsis and tuberculosis. During tuberculosis interactions between TLR2 and its ligands trigger immune responses that are important for bacterial control. Additionally TLR2’s overactivation links to sepsis by causing excessive inflammation. Researchers study TLR2 for potential therapeutic targeting in these diseases assessing how modulation can improve outcomes or reduce detrimental inflammation.

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Product protocols

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Target data

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity) (PubMed : 15690042). May also promote apoptosis in response to lipoproteins (By similarity). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2 : TLR6 : CD14 : CD36 in response to diacylated lipopeptides and TLR2 : TLR1 : CD14 in response to triacylated lipopeptides (By similarity). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (PubMed : 17486091). Acts as the major receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PhoS1 (pstS1), in conjunction with TLR1 and for some but not all lipoproteins CD14 and/or CD36. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed : 19362712). Recombinant MPT83 from M.tuberculosis stimulates secretion of cytokines (TNF-alpha, IL-6 and IL-12p40) by mouse macrophage cell lines in a TLR2-dependent fashion, which leads to increased host innate immunity responses against the bacterium (PubMed : 22174456). Lung macrophages which express low levels of TLR2 respond poorly to stimulation by M.tuberculosis LpqH (PubMed : 19362712). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM (PubMed : 27220037). Interacts with TICAM2 (By similarity).. (Microbial infection) Mediates activation of bone marrow-derived dendritic cells and macrophages, and production of pro-inflammatory cytokines, such as IL12 (IL12B/IL12A), triggered by Toxoplasma gondii micronemal protein 4 (MIC4) and micronemal protein 1 (MIC1).

See full target information Tlr2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 63:5 PubMed34985498

2022

Microbial Signatures in The Rodent Eyes With Retinal Dysfunction and Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ram Prasad,Bright Asare-Bediko,Angela Harbour,Jason L Floyd,Dibyendu Chakraborty,Yaqian Duan,Regina Lamendella,Justin Wright,Maria B Grant

Journal of healthcare engineering 2022:1473597 PubMed35035808

2022

Study on Toll-Like Receptor 2-Mediated Inflammation-Induced Familial Hypertension Combined with Hyperlipemia and Its Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Liu,Chunjing Li,Qiuyang Wang,Haiyan Hu,Chunhong Li,Jiuguang Qian

Cells 10: PubMed33924148

2021

Bone Marrow-Derived IL-1Ra Increases TNF Levels Poststroke.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Ulrich von Linstow,Sofie Mozart Hindkjær,Pernille Vinther Nielsen,Matilda Degn,Kate Lykke Lambertsen,Bente Finsen,Bettina Hjelm Clausen

Cell reports 33:108233 PubMed33027646

2020

Hyperactivity of Innate Immunity Triggers Pain via TLR2-IL-33-Mediated Neuroimmune Crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Junting Huang,Maria A Gandini,Lina Chen,Said M'Dahoma,Patrick L Stemkowski,Hyunjae Chung,Daniel A Muruve,Gerald W Zamponi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse TLR2 ELISA Kit

Sandwich ELISA - Mouse TLR2 ELISA Kit (AB224880)

Sandwich ELISA - Mouse TLR2 ELISA Kit (AB224880)

Sandwich ELISA - Mouse TLR2 ELISA Kit (AB224880)

Sandwich ELISA - Mouse TLR2 ELISA Kit (AB224880)

Sandwich ELISA - Mouse TLR2 ELISA Kit (AB224880)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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