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Mouse TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse TNF alpha in Mouse in serum free media, Plasma, Cell culture supernatant samples.
Alternative names=Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tnfa, Tnf, Tnfsf2
- Detection method
Colorimetric
- Sample types
serum free media, Plasma, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
93.75 - 6000 pg/mL
- Sensitivity
< 60 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed:25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity).The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
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Mouse TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse TNF alpha in Mouse in serum free media, Plasma, Cell culture supernatant samples.
Alternative names=Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tnfa, Tnf, Tnfsf2
Key facts
- Detection method
Colorimetric
- Sample types
serum free media, Plasma, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Mouse
- Range
93.75 - 6000 pg/mL
- Assay Platform
Microplate
- Sensitivity
< 60 pg/mL
- General recovery
93%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 92.46 | Range 84 - 104 % |
Sample type Plasma | Average % = 93.82 | Range 83 - 102 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's TNF alpha Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF alpha in plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Mouse TNFa levels are extremely low, and as such we expect "normal" levels to be undetectable when using our kit on plasma samples.
We have not been able to detect endogenous Mouse TNF alpha in normal serum with ab100747, only in serum spiked with Mouse TNF alpha.
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