Mouse TNF alpha ELISA Kit is a sandwich ELISA designed to quantify Mouse TNF alpha with a sensitivity of 60 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 93.75 - 6000 pg/mL
- Cited in over 70 publications
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- Neurotoxicity research 41:615-6262023Effect of Cannabidiol in LPS-Induced Toxicity in Astrocytes: Possible Role for Cannabinoid Type-1 Receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHind Ibork,Sara El Idrissi,Simo Siyanda Zulu,Robert Miller,Lhoussain Hajji,Annabelle Manalo Morgan,Khalid Taghzouti,Oualid AbboussiPubMed 37782433
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e23021302023Palmitoyltransferase ZDHHC3 Aggravates Nonalcoholic Steatohepatitis by Targeting S-Palmitoylated IRHOM2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinxuan Xu,Jun Tan,Liancai Zhu,Chenxu Ge,Yi Zhang,Fufeng Gao,Xianling Dai,Qin Kuang,Jie Chai,Benkui Zou,Bochu WangPubMed 37544908
- Acta pharmaceutica Sinica. B 13:1071-10922023The deubiquitinating enzyme 13 retards non-alcoholic steatohepatitis blocking inactive rhomboid protein 2-dependent pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinxuan Xu,Jun Tan,Liancai Zhu,Chenxu Ge,Wei Dong,Xianling Dai,Qin Kuang,Shaoyu Zhong,Lili Lai,Chao Yi,Qiang Li,Deshuai Lou,Linfeng Hu,Xi Liu,Gang Kuang,Jing Luo,Jing Feng,Bochu WangPubMed 36970206
- The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology 34:525-5322023Rhein Exhibits Anti-Inflammatory Effects in Chronic Atrophic Gastritis via Nrf2 and MAPK Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Liu,Lei Shu,Jiayao Yang,Yi Liu,Shu Zhang,Nian Wang,Zhaohong ShiPubMed 36789982
- Oxidative medicine and cellular longevity 2022:83854562022Cefazolin/BMP-2-Loaded Mesoporous Silica Nanoparticles for the Repair of Open Fractures with Bone Defects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingkui Shen,Lulu Wang,Li Feng,Chuangye Xu,Yi Gao,Sijing Li,Yulan Wu,Guoxian PeiPubMed 36193077
- Allergologia et immunopathologia 50:84-902022Knockdown of EDA2R alleviates hyperoxia-induced lung epithelial cell injury by inhibiting NF-κB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Jia,Yi Jia,Fen Yang,Wenchao DuPubMed 36086968
- Computational and mathematical methods in medicine 2022:93360802022Effects of Tea Treatments against High-Fat Diet-Induced Disorder by Regulating Lipid Metabolism and the Gut Microbiota.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Wang,Jiaxing Liu,Siyao Sang,Xue Ao,Mingjie Su,Binwei Hu,Hui LiPubMed 35677179
- Journal of neuroinflammation 19:1222022Intermittent fasting reduces neuroinflammation in intracerebral hemorrhage through the Sirt3/Nrf2/HO-1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuhui Dai,Jialiang Wei,Hongchen Zhang,Peng Luo,Yuefan Yang,Xiaofan Jiang,Zhou Fei,Wenbin Liang,Jianli Jiang,Xia LiPubMed 35624490
- Bioengineered 13:12726-127372022Knockout of circRNA single stranded interacting protein 1 (circRBMS1) played a protective role in myocardial ischemia-reperfusion injury though inhibition of miR-2355-3p/Mammalian Sterile20-like kinase 1 (MST1) axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingping Liang,Huanhuan Jie,Qin Liu,Chang Li,Renjie Xiao,Xianliang Xing,Jing Sun,Shuchun Yu,Yanhui Hu,Guo-Hai XuPubMed 35611768
- Molecules (Basel, Switzerland) 27:2022Anti-Inflammatory Effects of Leaf Extract and Isolated Lignans in RAW 264.7 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSupachoke Mangmool,Chayaporn Limpichai,Khine Kyi Han,Vichai Reutrakul,Natthinee AnantachokePubMed 35630789
Key facts
- Detection method
- Colorimetric
- Sample types
- serum free media, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 93.75 - 6000 pg/mL
- Sensitivity
- < 60 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed:25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (PubMed:32741026). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Alternative names
Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a
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Mouse TNF alpha ELISA Kit is a sandwich ELISA designed to quantify Mouse TNF alpha with a sensitivity of 60 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 93.75 - 6000 pg/mL
- Cited in over 70 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- serum free media, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Mouse
- Range
- 93.75 - 6000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 60 pg/mL
- General recovery
- 93%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 92.46 | Range 84 - 104 % |
Sample type Plasma | Average % = 93.82 | Range 83 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's TNF alpha Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF alpha in plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Mouse TNFa levels are extremely low, and as such we expect "normal" levels to be undetectable when using our kit on plasma samples.
We have not been able to detect endogenous Mouse TNF alpha in normal serum with ab100747, only in serum spiked with Mouse TNF alpha.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab100747)
TNF-alpha was measured in cell culture medium from Raw control cells, or cells stimulated with PMA (10 ng x mL-1; 24 h) and LPS (1 ug x mL-1; 6 h). Results are shown with background signal subtracted (duplicates +/- SD).
Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab100747)
Representative Standard Curve using ab100747
Sandwich ELISA - Mouse TNF alpha ELISA Kit (ab100747)
Representative Standard Curve using ab100747
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