JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB208348

Mouse TNF alpha ELISA Kit

4

(4 Reviews)

|

(238 Publications)

Mouse TNF alpha ELISA kit is a single-wash, 90-minute SimpleStep ELISA® designed to quantify mouse TNF alpha with a sensitivity of 9.1 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA -450 nm readout; works on any plate reader
- Different formats for different needs – 384-well format for higher throughput
- Broad sample compatibility – works with serum, plasma (heparin, citrate, EDTA), and cell culture supernatants
- Cited in over 235 publications

View Alternative Names

Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

14 Images
Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)
  • sELISA

Lab

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

Interpolated concentrations of spiked TNF alpha in mouse plasma samples.

The concentrations of TNF alpha were measured in duplicates, interpolated from the TNF alpha standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF alpha concentration was determined to be 1.68 ng/mL in plasma (citrate), 1.81 ng/mL in plasma (EDTA) and 1.47 ng/mL in plasma (heparin).

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

Example of mouse TNF alpha standard curve in Sample Diluent 25BS.

The TNF alpha standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

Interpolated concentrations of native TNF alpha in mouse RAW 264.7 LPS stimulated and unstimulated cell culture supernatant samples.

The concentrations of TNF alpha were measured in duplicates, interpolated from the TNF alpha standard curves and corrected for sample dilution. Undiluted samples are as follows : RAW 264.7 LPS stimulated supernatant 50% and RAW 264.7 LPS unstimulated supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF alpha concentration was determined to be 6346.4 pg/mL in RAW 264.7 LPS stimulated supernatant and 83.7 pg/mL in RAW 264.7 LPS unstimulated supernatant. RAW 264.7 cells were cultured in HGDMEM with 100 μg/mL Kanamycin and 2 mM L-glutamine. Cells were starved for 24 hours and treated in the presence and absence of 5 μg/mL of LPS.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

Example of mouse TNF alpha standard curve in Sample Diluent 25BS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

Assay sensitivity.

The calculated minimal detectable dose (MDD) is determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentrations. The MDD is dependent on the Sample Diluent buffer used.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

Interpolated concentrations of spiked mouse TNF alpha in mouse serum and cell culture samples and native mouse TNF alpha in mouse RAW 264.7 LPS stimulated cell culture supernatants.

The concentrations of TNF alpha were measured in duplicates, interpolated from the TNF alpha standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, cell culture 100%, and RAW 264.7 LPS stimulated cell culture supernatants 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNF alpha concentration was determined to be 1.63 ng/mL in serum, 1.00 ng/mL in cell culture and 6.07 ng/mL in RAW 264.7 LPS stimulated cell culture supernatants.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

Example of mouse TNF alpha standard curve in Sample Diluent NS.

The TNF alpha standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

Example of mouse TNF alpha standard curve in Sample Diluent 10BS.

The TNF alpha standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

Example of mouse TNF alpha standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

Example of mouse TNF alpha standard curve in Sample Diluent 10BS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

Example of mouse TNF alpha standard curve in Sample Diluent 25BS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

Example of mouse TNF alpha standard curve in Sample Diluent 10BS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB208348)

Example of mouse TNF alpha standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 9.1 pg/mL

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

You may be interested in:

AB241672

Mouse TNF alpha Antibody Pair - BSA and Azide free

0

0 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column

Product details

Mouse TNF alpha ELISA Kit ab208348 is a rapid single-wash 90-min ELISA kit to measure cell culture supernatant, Serum, plasma. This SimpleStep ELISA® sensitivity is 9.1 pg/mL.

How the assay works

SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant mouse TNF alpha protein in serum, plasma, and cell culture supernatant samples only.
Recombinant mouse TNF RI, TNF RII, OPG, CD40 receptor, IL1 beta, IL-16, and IL-5 were prepared at 100 ng/mL and assayed for cross reactivity. No cross-reactivity was observed. Recombinant human TNF alpha was prepared at 100 ng/mL, 25 ng/mL, and 3 ng/mL and assayed for cross reactivity. On average, 3.0% cross-reactivity was observed with a standard deviation of 0.7%.
Recombinant mouse TNF RI, TNF RII, OPG, CD40 receptor, IL1 beta, IL-16, and IL-5 and recombinant human TNF alpha were prepared at 100 ng/mL and tested for interference. No interference with was observed.
This immunoassay is calibrated against a highly purified mouse TNF alpha. The NIBSC/WHO unclassified purified mouse TNF alpha preparation 88/532 was evaluated in this kit. The dose response curve of the unclassified standard 88/532 parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep mouse TNF alpha kit to approximate NIBSC 88/532 units, use the equation below.
NIBSC (88/532) approximate value (units/mL) = 2.0 x SimpleStep mouse TNF alpha value (pg/mL)

Mouse TNF alpha ELISA Kit ab208348 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse TNF alpha ELISA Kit ab208348

Mouse TNF alpha ELISA Kit ab208348 has been used to assess inflammation in different physio-pathological contexts such as liver disease(1), aging(2), and Alzheimer's disease(3).

References:

(1)Ai-Lei Xu et al. 2023, PMID: 37902450,
(2)Yang Bao et al. 2023, PMID: 37954634,
(3)Ramon Velazquez et al. 2023, PMID: 37548694

Save your precious samples

Our 384-well Mouse TNF alpha ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Mouse TNF alpha Antibody Pair - BSA and Azide free ab241672
- Anti-TNF alpha antibody [EPR16803-2] - BSA and Azide free (Capture) ab242949
- Anti-TNF alpha antibody [EPR16803-84] - BSA and Azide free (Detector) ab242977

Related and recommended products

Mouse TNF alpha ELISA kit ab208348 is commonly used to measure TNF alpha as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:

- Mouse IL-6 ELISA kit ab222503
- Mouse IFN gamma ELISA kit ab282874
- Mouse IL-1 beta ELISA kit ab197742

Tumor necrosis factor, or TNF alpha, is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. Furthermore, TNF alpha is a ligand of the TNF superfamily which plays a central role in inflammation, apoptosis, proliferation, invasion, angiogenesis, metastasis, and morphogenesis. It is mainly secreted by macrophages, and can induce cell death of certain tumor cell lines. Rat and Human TNF alpha are 95% and 79% identical to Mouse TNF alpha, respectively. TNF alpha is expressed on macrophages and endothelial, epithelial, and tumor cells as a 26 kDa transmembrane protein. TNF-alpha is cleaved by proteolytic processing into six chains: (1) TNF membrane form, (2) Intracellular domain 1 (ICD1), (3) Intracellular domain 2 (ICD2), (4) C-domain 1, (5) C-domain 2 and (6) TNF soluble form. ICD1 and ICD2 are released into the cytosol, while C-domain 1 and C-domain 2 are released into the extracellular space. Furthermore, the membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. Signaling from TNF-alpha differs depending on the type of ligand initiating the signaling event (intracellular, membrane, or soluble). As an example, the membrane form of TNF-alpha appears to mediate anti-tumorigenic therapeutic responses whereas the soluble ligand is linked to inflammation and proliferation. Likewise, the TNF intracellular domain (ICD) form induces IL-12 production in dendritic cells.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "85 - 115 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "98 - 101 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "85 - 103 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "85 - 88 %", "average": "= 87" }, { "sample": "Cell culture media", "range": "109 - 115 %", "average": "= 111" } ]
style
left-column

What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab208348-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB208348-CMP06" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB208348-CMP12" }, { "size":"1 x 600 µL", "name":"10X Mouse TNF alpha Capture Antibody", "number":"AB208348-CMP08" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB208348-CMP11" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB208348-CMP03" }, { "size":"1 x 20 mL", "name":"Sample Diluent NBS", "number":"AB208348-CMP04" }, { "size":"2 x 1 Vial", "name":"Mouse TNF alpha Lyophilized Recombinant Protein", "number":"AB208348-CMP02" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB208348-CMP10" }, { "size":"1 x 600 µL", "name":"10X Mouse TNF alpha Detector Antibody", "number":"AB208348-CMP09" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB208348-CMP07" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB208348-CMP13" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab208348-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB208348-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse TNF alpha Capture Antibody", "number":"AB208348-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB208348-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB208348-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent NBS", "number":"AB208348-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB208348-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse TNF alpha Lyophilized Recombinant Protein", "number":"AB208348-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB208348-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse TNF alpha Detector Antibody", "number":"AB208348-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB208348-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB208348-CMP05", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed : 25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (PubMed : 32741026).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information Tnf
style
left-column

Publications (238)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 24:505-519 PubMed37902450

2024

Silencing METTL14 alleviates liver injury in non-alcoholic fatty liver disease by regulating mitochondrial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Jun Yan,Long Han,Zi-Lin Zou,Ai-Lei Xu

NPJ systems biology and applications 10:5 PubMed38218856

2024

The antidepressive mechanism of Longya Lilium combined with Fluoxetine in mice with depression-like behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Huina Ma,Hehua Huang,Chenyu Li,Shasha Li,Juefang Gan,Chunrong Lian,Yanwu Ling

Scientific reports 13:22409 PubMed38104182

2023

Chia seeds oil ameliorate chronic immobilization stress-induced neurodisturbance in rat brains via activation of the antioxidant/anti-inflammatory/antiapoptotic signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Norhan E Khalifa,Ahmed E Noreldin,Asmaa F Khafaga,Mohamed El-Beskawy,Eman Khalifa,Ali H El-Far,Abdel-Hasseb A Fayed,Abdeldayem Zakaria

The European journal of neuroscience 59:36-53 PubMed37985440

2023

Dexmedetomidine alleviates hippocampal neuronal loss and cognitive decline in rats undergoing open surgery under sevoflurane anaesthesia by suppressing CCAAT/enhancer-binding protein beta.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Fu,Xianghai Zhao,Yingna Li,Xinwen Fan,Zeqing Huang

Journal of inflammation research 16:5095-5109 PubMed38026263

2023

LPA Alleviates Septic Acute Lung Injury via Protective Endothelial Barrier Function Through Activation of PLC-PKC-FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Ruifeng Bai,Jianqiu Pei,Shengqiang Pei,Xiangfeng Cong,Jerold Chun,Fang Wang,Xi Chen

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 18:628-639 PubMed37919457

2023

GPNMB Ameliorates Neuroinflammation Via the Modulation of AMPK/NFκB Signaling Pathway After SAH in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Li,Yuansheng Zhang,Qixiong Lu,Li Lei,Jingshu Du,Xiaoyang Lu

Parasitology research 122:3213-3231 PubMed37874393

2023

Effects of metformin on parasitological, pathological changes in the brain and liver and immunological aspects during visceral toxocariasis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Amina M Salama,Rasha A Elmahy,Hoda A Ibrahim,Alaa Ibrahim Mohamed Amer,Asmaa Fawzy Eltantawy,Dina I Elgendy

Biomedical reports 19:100 PubMed37954634

2023

Dexmedetomidine attenuates the neuroinflammation and cognitive dysfunction in aged mice by targeting the SNHG14/miR‑340/NF‑κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guangbao He,Hongwei Ni,Kai Wang,Hongmei Gao,Yu Li,Jiaqiu Gu,Xin Ni,Zhiming Wang,Yang Bao

Molecular medicine (Cambridge, Mass.) 29:137 PubMed37858064

2023

Resveratrol reduces ROS-induced ferroptosis by activating SIRT3 and compensating the GSH/GPX4 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xingjie Wang,Tianli Shen,Jie Lian,Kai Deng,Chao Qu,Enmeng Li,Gan Li,Yiwei Ren,Zijun Wang,Zhengdong Jiang,Xuejun Sun,Xuqi Li

Heliyon 9:e20124 PubMed37771539

2023

IL-1β-pretreated bone mesenchymal stem cell-derived exosomes alleviate septic endoplasmic reticulum stress via regulating SIRT1/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinsheng Cheng,Shikai Wang,Zhipeng Li,Di He,Jianguo Wu,Weiwei Ding
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
websiteProtocolBooklet
en
style
right-column

Associated Products

Select an associated product type
Alternative Product
Assay Kits

AB100747

Mouse TNF alpha ELISA Kit

elisa-kits

mouse-tnf-alpha-elisa-kit-ab100747

4

(1 reviews)

Alternative Product
Assay Kits

AB108910

Mouse TNF alpha ELISA Kit

elisa-kits

mouse-tnf-alpha-elisa-kit-ab108910

0

(0 reviews)

Alternative Product
Assay Kits

AB46105

Mouse TNF alpha ELISA Kit

elisa-kits

mouse-tnf-alpha-elisa-kit-ab46105

4

(2 reviews)

Alternative Product
Assay Kits

AB229393

Mouse TNF alpha ELISA Kit, Fluorescent

elisa-kits

mouse-tnf-alpha-elisa-kit-fluorescent-ab229393

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com