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AB46105

Mouse TNF alpha ELISA Kit

4

(2 Reviews)

|

(15 Publications)

Mouse TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse TNF alpha in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

1 Images
Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB46105)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF alpha ELISA Kit (AB46105)

Representative Standard Curve using ab46105

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

< 25 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TNF alpha mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TNF alpha in mouse serum, buffered solutions and cell culture medium.

A monoclonal antibody specific for TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.

This kit will recognize both endogenous and recombinant mouse TNF alpha.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 10, "mean": "528.7 pg/mL", "standardDeviation": "37.3", "coefficientOfVariability": "7" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 10, "mean": "132.2 pg/mL", "standardDeviation": "10.8", "coefficientOfVariability": "8.1" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "553.5 pg/mL", "standardDeviation": "11.2", "coefficientOfVariability": "2" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "124 pg/mL", "standardDeviation": "6.4", "coefficientOfVariability": "5.1" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed : 25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (PubMed : 32741026).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information Tnf
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:977 PubMed40452015

2025

Cholesterol-induced colorectal cancer progression and its mitigation through gut microbiota remodeling and simvastatin treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Xie,Wenjing Wang,Haiming Zhang,Shaohui Zhao,Na Zhang,Ying Gao,Quanxia Liu,Xiaomei Chen

Cellular and molecular life sciences : CMLS 81:4 PubMed38070059

2023

Myeloid SENP3 deficiency protects mice from diet and age-induced obesity via regulation of YAP1 SUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yangjing Jiang,Min Liang,Long Chen,Jian Wang,Yijie Huang,Huanhuan Huo,Danrui Xiao,Yunwen Hu,Zi Wang,Qingqi Ji,Yanjie Li,Zhaohua Cai,Ben He

Experimental and therapeutic medicine 23:303 PubMed35340877

2022

Sevoflurane preconditioning prevents acute renal injury caused by ischemia-reperfusion in mice via activation of the Nrf2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Xi Wang,Zhen-Ru Zhao,Ying Bai,Ya-Xing Li,Xiao-Ning Gao,Sen Zhang,Yan-Bin Sun

iScience 24:103262 PubMed34755095

2021

Role of adipocyte Na,K-ATPase oxidant amplification loop in cognitive decline and neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Komal Sodhi,Rebecca Pratt,Xiaoliang Wang,Hari Vishal Lakhani,Sneha S Pillai,Mishghan Zehra,Jiayan Wang,Lawrence Grover,Brandon Henderson,James Denvir,Jiang Liu,Sandrine Pierre,Thomas Nelson,Joseph I Shapiro

Psychopharmacology 238:1979-1990 PubMed33774704

2021

Do proinflammatory cytokines play a role in clozapine-associated glycometabolism disorders?

Applications

Unspecified application

Species

Unspecified reactive species

Tongtong Zhao,Kai Zhang,Yelei Zhang,Yating Yang,Xiaoshuai Ning,Yu Hu,Xiaoyue Li,Yulong Zhang,Lei Xia,Zhenhua Ren,Huanzhong Liu

Frontiers in endocrinology 11:91 PubMed32218769

2020

A Novel Indoline Derivative Ameliorates Diabesity-Induced Chronic Kidney Disease by Reducing Metabolic Abnormalities.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Permyakova,Asaad Gammal,Liad Hinden,Michal Weitman,Marta Weinstock,Joseph Tam

International journal of molecular sciences 20: PubMed31842453

2019

Effects of Farnesiferol B on Ischemia-Reperfusion-Induced Renal Damage, Inflammation, and NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhang,Xianjun Fu,Ting Gui,Tianqi Wang,Zhenguo Wang,Gerd A Kullak-Ublick,Zhibo Gai

Molecular medicine reports 20:1321-1332 PubMed31173225

2019

Artesunate exerts protective effects against ulcerative colitis via suppressing Toll‑like receptor 4 and its downstream nuclear factor‑κB signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Xuan Chen,Xiao-Qi Zhang,Cheng-Gong Yu,Shu-Ling Huang,Ying Xie,Xiao-Tan Dou,Wen-Jia Liu,Xiao-Ping Zou

Frontiers in pharmacology 9:372 PubMed29725297

2018

1-Deoxynojirimycin (DNJ) Ameliorates Indomethacin-Induced Gastric Ulcer in Mice by Affecting NF-kappaB Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xuehua Piao,Shuangdi Li,Xiaodan Sui,Lianyi Guo,Xingmei Liu,Hongmei Li,Leming Gao,Shusheng Cai,Yanrong Li,Tingting Wang,Baohai Liu

Frontiers in pharmacology 9:347 PubMed29695964

2018

Root Extract of Ameliorates DSS-Induced Ulcerative Colitis by Affecting NF-kappaB Signaling Pathway in a Mouse Model via Synergistic Effects of Polydatin, Resveratrol, and Emodin.

Applications

Unspecified application

Species

Unspecified reactive species

Baohai Liu,Shuangdi Li,Xiaodan Sui,Lianyi Guo,Xingmei Liu,Hongmei Li,Leming Gao,Shusheng Cai,Yanrong Li,Tingting Wang,Xuehua Piao
View all publications
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