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AB320046

Mouse TNF beta ELISA Kit

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Mouse TNF beta ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse TNF beta with a sensitivity of 2.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Tnfb, Tnfsf1, Lta, Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1

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Sandwich ELISA - Mouse TNF beta ELISA Kit (AB320046)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF beta ELISA Kit (AB320046)

Interpolated concentration of native TNF beta was measured in duplicate at different sample concentrations. Undiluted samples are 50% treated mouse spleen supernatant (treated with PMA 40 ng/mL + calcium ionomycin 500 ng/mL for 3d) and 50% untreated mouse spleen supernatant.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse TNF beta ELISA Kit (AB320046)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF beta ELISA Kit (AB320046)

Interpolated concentration of native TNF beta was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% mouse spleen supernatant (treated with PMA 40 ng/mL + calcium ionomycin 500 ng/mL for 3d).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS

Sandwich ELISA - Mouse TNF beta ELISA Kit (AB320046)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF beta ELISA Kit (AB320046)

Example of mouse TNF beta standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse TNF beta ELISA Kit (AB320046)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNF beta ELISA Kit (AB320046)

Example of mouse TNF beta standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant, Heparin Plasma

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.7 pg/mL

Range

21.094 - 1350 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse TNF beta SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TNF beta protein in Serum, Cit plasma, EDTA Plasma, Hep Plasma, and Cell culture supernatant. Quantitate Mouse TNF beta with 2.7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]
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Recovery

[ { "sample": "Serum", "range": "85 - 95 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "92 - 92 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "85 - 89 %", "average": "= 86" }, { "sample": "Heparin Plasma", "range": "96 - 100 %", "average": "= 98" }, { "sample": "Cell culture supernatant", "range": "91 - 111 %", "average": "= 100" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (By similarity). In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.
See full target information Lta
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com