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AB213895

Mouse TNFRSF7 ELISA Kit (CD27)

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Mouse TNFRSF7 ELISA Kit (CD27) is a Sandwich ELISA for the measurement of Mouse TNFRSF7 (CD27) in Mouse in Cell Culture Media, Biofluids samples.

View Alternative Names

CD27, Tnfrsf7, Cd27, CD27 antigen, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7

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Sandwich ELISA - Mouse TNFRSF7 ELISA Kit (CD27) (AB213895)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TNFRSF7 ELISA Kit (CD27) (AB213895)

Mouse TNFRSF7 ELISA Kit (CD27) (ab213895) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Proteins & Peptides

AB316470

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Mouse TNFRSF7 Enzyme-Linked Immunosorbent Assay (ELISA) kit (CD27) (ab213895) is designed for the quantitative detection of Mouse TNFRSF7 in cell culture supernatants and serum.

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for TNFRSF7/CD27 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: T21-R182) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF7/CD27 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TNFRSF7/CD27 amount of sample captured in plate.

CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "658 pg/mL", "standardDeviation": "39.5", "coefficientOfVariability": "6" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "1856 pg/mL", "standardDeviation": "89.1", "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "2731 pg/mL", "standardDeviation": "180.3", "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "533 pg/mL", "standardDeviation": "22.4", "coefficientOfVariability": "4.2" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "1626 pg/mL", "standardDeviation": "56.91", "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "2563 pg/mL", "standardDeviation": "138.4", "coefficientOfVariability": "5.4" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD27 also known as Tumor Necrosis Factor Receptor Superfamily Member 7 (TNFRSF7) is a protein chiefly involved in T cell activation and proliferation. It belongs to the TNF-receptor superfamily and weighs approximately 50 kDa. CD27 is expressed mainly on T lymphocytes and to a lesser extent on B cells and natural killer cells. Researchers often study CD27 and its ligand CD70 to understand immune responses. Tools such as anti-CD27 percp 7h21 monoclonal antibodies and APC-conjugated forms help detect this protein in various assays.
Biological function summary

One finds CD27 at the heart of cellular immune responses. It does not function alone; instead it typically forms part of a receptor-ligand complex with CD70 which influences T cell and B cell activities. This interaction promotes cell survival and plays a role in the activation of NF-kB and MAPK8/JNK signaling pathways which are essential for immune response regulation. These pathways ensure that the body's immune cells can efficiently respond to pathogens.

Pathways

CD27 actively integrates into the NF-kB and mTOR signaling pathways which are important for regulating immune and inflammatory responses. Through these pathways CD27 works closely with proteins like TRAF2 and TRAF3 which are adapter molecules that transmit signals from the receptor. These signaling pathways provide a framework for understanding how CD27 and associated proteins work together to control immune cell behavior facilitating coordinated immune defense mechanisms.

CD27 has significant implications in autoimmune diseases and cancer. For example aberrant activation of CD27 may contribute to conditions like lymphoma and autoimmune diseases such as lupus. The CD70-CD27 signaling axis when dysregulated can lead to uncontrolled cell proliferation or apoptosis linking it to these pathologies. Understanding the interplay between CD27 and proteins like Bcl-2 which influences apoptosis helps pave the way for therapeutic strategies targeting these diseases.
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Product protocols

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Target data

Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells (PubMed : 11062504). Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (By similarity). In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis (PubMed : 15723067). Negatively regulates the function of Th2 lymphocytes in the adipose tissue (PubMed : 38386557).
See full target information Cd27
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websiteProtocolBooklet
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