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AB193729

Mouse TNFSF4 ELISA Kit

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(1 Publication)

Mouse TNFSF4 ELISA Kit is a Sandwich ELISA for the measurement of Mouse TNFSF4 in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

CD252, Ox40l, Txgp1l, Tnfsf4, Tumor necrosis factor ligand superfamily member 4, OX40 ligand, OX40L

1 Images
Sandwich ELISA - Mouse TNFSF4 ELISA Kit (AB193729)
  • sELISA

Unknown

Sandwich ELISA - Mouse TNFSF4 ELISA Kit (AB193729)

Example of typical mouse TNFSF4 standard curve

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 30 pg/mL

Range

30 - 8000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TNFSF4 Mouse ELISA Kit (ab193729) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TNFSF4 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse TNFSF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures TNFSF4 present in the samples. The wells are washed and biotinylated anti-mouse TNFSF4 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of TNFSF4 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "99 - 137 %", "average": "= 116.8" }, { "sample": "Plasma", "range": "87 - 122 %", "average": "= 103.3" }, { "sample": "Serum", "range": "85 - 127 %", "average": "= 106.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193729-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193729-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193729-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193729-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193729-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated mouse TNFSF4 Microplate (12 strips x 8 wells)", "number":"AB193729-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Mouse TNFSF4 detection antibody", "number":"AB193729-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"mouse TNFSF4 standards (lyophilized)", "number":"AB193729-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193729-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB193729-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OX40L also known as TNFSF4 is a member of the tumor necrosis factor (TNF) ligand family. It is a type II transmembrane protein with a molecular weight of about 32 kDa. OX40L is expressed mainly on activated antigen-presenting cells such as B cells dendritic cells and macrophages. The protein interacts specifically with its receptor OX40 (CD134) which is found on activated T cells mainly CD4+ T cells. These interactions are important for the regulation of immune responses.
Biological function summary

OX40L acts as a costimulatory signal for T cells. It does not work alone; it functions as part of the immune complex involved in enhancing T cell proliferation cytokine production and survival. OX40L enhances the immune response making it a critical modulator of immunity. This protein interaction is essential for promoting an effective adaptive immune response influencing T cell memory and maintaining immune system balance.

Pathways

OX40L is involved in the TNF receptor pathway which plays an important role in cell survival and inflammation. It influences the NF-κB signaling pathway which regulates immune response and cell proliferation. OX40L interactions also involve the Akt pathway important for T cell survival and function. The related proteins in these pathways include notably the OX40 protein and CD317 which participate in immune signaling and regulation.

OX40L is implicated in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. The interaction between OX40L and OX40 increases T cell activation contributing to inflammation and disease progression. OX40L's excessive activity has links to these pathologies making it a potential therapeutic target. Another related protein within disease contexts is CD317 connected to immune modulation and control of viral infections and offering further opportunities for targeted treatment strategies involving OX40L.
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Product protocols

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Target data

Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
See full target information Tnfsf4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 41:185-199 PubMed30773478

2019

DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangqi Meng,Chunbin Duan,Hengyuan Pang,Qun Chen,Bo Han,Caijun Zha,Magafurov Dinislam,Pengfei Wu,Ziwei Li,Shihong Zhao,Ruijia Wang,Lin Lin,Chuanlu Jiang,Jinquan Cai
View all publications
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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