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AB285242

Mouse Total cholesterol ELISA Kit

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(5 Publications)

Mouse Total cholesterol ELISA Kit is a sandwich ELISA designed to quantify Mouse Total cholesterol

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 78 - 5000 pg/mL
1 Images
Sandwich ELISA - Mouse Total cholesterol ELISA Kit (AB285242)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Total cholesterol ELISA Kit (AB285242)

Mouse Total Cholesterol ELISA Kit.

Typical Standard Curve : These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich

Results type

Qualitative

Range

78 - 5000 pg/mL

Assay time

90m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Total cholesterol ELISA Kit ab285242 is a sandwich ELISA to measure Mouse Total cholesterol in serum, plasma, cell culture supernatant, tissue homogenate, other biological fluids.

How the assay works

The entire process can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Total cholesterol ELISA Kit ab285242 protocol summary

1. Add standards or samples to appropriate wells. Incubate
2. Wash
3. Add HRP conjugate reagent to all wells. Incubate
4. Wash
5. Add Chromogen solutions to each well. Incubate.
6. Add stop solution and read at 450 nm.

This product is manufactured by BioVision, an Abcam company and was previously called K4440 QuickDetect™ Total cholesterol (Mouse) ELISA Kit. K4440-100 is the same size as the 96 test size of ab285242.

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab285242-96Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Micro ELISA Plate", "number":"AB285242-CMP08", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Microplate Sealers", "number":"AB285242-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"20X Wash buffer", "number":"AB285242-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Standard diluent", "number":"AB285242-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Stop Solution", "number":"AB285242-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"HRP-Conjugate Reagent", "number":"AB285242-CMP03", "productcode":"" }, { "size":"1 x 0.4 mL", "name":"Standard (5000 pg/ml)", "number":"AB285242-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Chromogen Solution B", "number":"AB285242-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Special diluent", "number":"AB285242-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Chromogen Solution A", "number":"AB285242-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cholesterol sometimes referred to as fchol is a lipid molecule with a molecular mass of approximately 386.65 Daltons. It is an essential component of cell membranes and is found in the plasma membranes of cells throughout the body. Cholesterol primarily resides in the membranes where it modulates fluidity and mechanical properties. Its expression occurs in various tissues but it is abundant in the liver which plays an important role in its synthesis and regulation.
Biological function summary

Cholesterol is important in maintaining the structural integrity and organization of membranes. It is also part of lipid rafts which are specialized microdomains within the cell membrane that facilitate cellular signaling. Cholesterol interacts with proteins and receptors embedded in the membrane influencing their function. It does not form a complex itself but affects complexes within the membrane like lipid rafts and signaling platforms.

Pathways

Cholesterol is a critical component of the steroid biosynthesis pathway and the bile acid synthesis pathway. It serves as a precursor for steroid hormones including cortisol and sex hormones as well as bile acids which are essential for digestion and lipid absorption. Proteins such as HMG-CoA reductase and the low-density lipoprotein receptor (LDLR) are key players related to cholesterol in these pathways regulating its synthesis and uptake in cells.

Cholesterol is closely associated with atherosclerosis and cardiovascular disease. Elevated levels of low-density lipoprotein (LDL) cholesterol contribute to plaque formation in arteries increasing the risk of heart attack and stroke. On the other hand high-density lipoprotein (HDL) cholesterol helps remove excess cholesterol from tissues including the arterial walls. Statins a class of drugs that lower LDL cholesterol levels target HMG-CoA reductase and are commonly used in treating hypercholesterolemia and cardiovascular diseases.
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Product protocols

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Target data

See full target information Cholesterol
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1484278 PubMed39654627

2024

Osteopontin neutralization increases vitamin D receptors on NKT cells and ameliorates liver fibrosis by promoting their activity.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Amer,Ahmad Salhab,Enas Hussini,Rasha Shweiki,Iman Zahran,Mohammad Far

Frontiers in pharmacology 14:1219709 PubMed37614321

2023

The immune and metabolic treatment approach of using testosterone on mice models of liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Amer,Ahmad Salhab,Hadeel Snobar,Yazan Alhabil

Research (Washington, D.C.) 2022:0001 PubMed39285950

2022

PGAM5-Mediated PHB2 Dephosphorylation Contributes to Diabetic Cardiomyopathy by Disrupting Mitochondrial Quality Surveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Rongjun Zou,Jun Tao,Jie He,Chaojie Wang,Songtao Tan,Yu Xia,Xing Chang,Ruibing Li,Ge Wang,Hao Zhou,Xiaoping Fan

Scientific reports 12:13712 PubMed35962139

2022

Nicotinamide mononucleotide ameliorates adriamycin-induced renal damage by epigenetically suppressing the NMN/NAD consumers mediated by Twist2.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuhiro Hasegawa,Yusuke Sakamaki,Masanori Tamaki,Shu Wakino

Frontiers in pharmacology 13:875549 PubMed35833020

2022

Theabrownin and Polysaccharide Improve Lipid Metabolism Modulation of Bile Acid and Fatty Acid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Jieyi Wang,Dan Zheng,Fengjie Huang,Aihua Zhao,Junliang Kuang,Zhenxing Ren,Tianlu Chen,Jing Lei,Jingchao Lin,Xiaoning Wang,Wei Jia,Guoxiang Xie,Xiaojiao Zheng
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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