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AB193730

Mouse TRAIL ELISA Kit

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(1 Publication)

Mouse TRAIL ELISA Kit is a Sandwich ELISA for the measurement of Mouse TRAIL in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

CD253, Trail, Tnfsf10, Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL

2 Images
Sandwich ELISA - Mouse TRAIL ELISA Kit (AB193730)
  • sELISA

Unknown

Sandwich ELISA - Mouse TRAIL ELISA Kit (AB193730)

Example of typical mouse TRIAL standard curve using 1x Assay diluent B

Sandwich ELISA - Mouse TRAIL ELISA Kit (AB193730)
  • sELISA

Unknown

Sandwich ELISA - Mouse TRAIL ELISA Kit (AB193730)

Example of typical mouse TRIAL standard curve using assay diluent A

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2 pg/mL

Range

2 - 600 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TRAIL (TNF-related apoptosis-inducing ligand) Mouse ELISA Kit (ab193730) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TRAIL in serum, plasma and cell culture supernatants. (GeneCards: TNFRSF10D)

This assay employs an antibody specific for mouse TRAIL coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures TRAIL present in the samples. The wells are washed and biotinylated anti-mouse TRAIL antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of TRAIL bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "115 - 129 %", "average": "= 122.5" }, { "sample": "Plasma", "range": "76 - 104 %", "average": "= 88.25" }, { "sample": "Serum", "range": "91 - 104 %", "average": "= 97.38" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193730-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193730-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193730-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Mouse TRAIL detection antibody", "number":"AB193730-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"mouse TRAIL standards (lyophilized)", "number":"AB193730-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193730-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated mouse TRAIL Microplate", "number":"AB193730-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin Concentrate", "number":"AB193730-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193730-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB193730-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRAIL also known as TNF-related apoptosis-inducing ligand plays an important role in inducing apoptosis. This protein has a mass of around 32 kDa and is expressed across various tissues including immune cells. TRAIL interacts with specific receptors on the cell surface to initiate a signaling cascade that results in programmed cell death. It is an attractive therapeutic target because of its selective ability to induce apoptosis in cancer cells while sparing most normal cells.
Biological function summary

TRAIL's function extends beyond inducing cell death. It is an important member of the tumor necrosis factor (TNF) superfamily which regulates immune responses and inflammation. TRAIL can form a homotrimeric complex allowing it to bind effectively to death receptors on target cells specifically DR4 and DR5 triggering a series of downstream signaling events. This interaction is significant in modulating immune surveillance and tumor suppression.

Pathways

TRAIL is integral to both intrinsic and extrinsic apoptosis pathways. Through these pathways TRAIL is closely related to FADD and caspase-8 which are important for apoptosis induction. The extrinsic pathway involves binding to TRAIL death receptors leading to DISC formation while the intrinsic pathway involves mitochondrial events mediated by members of the Bcl-2 protein family.

TRAIL has a significant connection with cancer and autoimmune diseases. TRAIL plays a dual role in cancer where it acts as a tumor suppressor but can also promote tumor progression if cancer cells become resistant to its effects. In cancer TRAIL’s interaction with proteins like FLIP can alter the apoptotic potential of the cells. In autoimmune diseases TRAIL's dysregulation can contribute to tissue damage due to increased immune activity highlighting its complex role in maintaining immune balance.
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Product protocols

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Target data

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
See full target information Tnfsf10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular medicine (Berlin, Germany) 97:1247-1261 PubMed31230087

2019

Preclinical studies of a death receptor 5 fusion protein that ameliorates acute liver failure.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Chen,Pu Wang,Qingmei Zhang,Meng Xia,Guizhong Zhang,Junxin Li,Enyun Shen,Youhai H Chen,Xiaochun Wan
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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