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AB157724

Mouse Transferrin ELISA Kit

4

(1 Review)

|

(8 Publications)

Mouse Transferrin ELISA Kit is a sandwich ELISA designed to quantify Mouse Transferrin with a sensitivity of 0.6935 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 3.13 - 100 ng/mL

View Alternative Names

Trf, Tf, Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin

1 Images
Sandwich ELISA - Mouse Transferrin ELISA Kit (AB157724)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Transferrin ELISA Kit (AB157724)

Representative standard curve using ab157724 Transferrin Mouse ELISA Kit.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 0.6935 ng/mL

Range

3.13 - 100 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse Transferrin ELISA Kit ab157724 is a sandwich ELISA to measure Mouse Transferrin in serum, plasma with a sensitivity of 0.6935 ng/ml.

How the assay works

In this assay the Transferrin present in samples reacts with the anti-Transferrin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-Transferrin antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Transferrin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Transferrin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Transferrin in the test sample. The quantity of Transferrin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Transferrin ELISA Kit ab157724 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
6. Immediately begin recording the color development
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab157724-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Mouse Transferrin ELISA Microplate", "number":"AB157724-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Mouse Transferrin Calibrator (lyophilized)", "number":"AB157724-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB157724-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB157724-CMP04", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP-conjugated anti-mouse Transferrin antibody", "number":"AB157724-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB157724-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB157724-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transferrin also known as serotransferrin or siderophilin is a glycoprotein with a mass of approximately 80 kDa. It is primarily synthesized in the liver and subsequently secreted into the bloodstream. Transferrin has an important role in iron transport and maintains iron homeostasis by binding and delivering iron to various tissues throughout the body. It can bind two ferric ions (Fe3+) in association with an anion usually bicarbonate. In biological fluids transferrin exists in serum plasma and other extracellular fluids.
Biological function summary

Transferrin facilitates the transportation of iron ions. It delivers iron to cells by binding to transferrin receptors on cell surfaces forming a complex that gets internalized via receptor-mediated endocytosis. Inside the endosomes acidic conditions cause iron to release from transferrin enabling its utilization in cellular processes like DNA synthesis and electron transport. Transferrin itself acts independently and does not form part of a larger protein complex. Variants of transferrin include mouse transferrin bovine transferrin and biotinylated transferrin each with similar function across different species.

Pathways

Transferrin operates centrally in iron metabolism and homeostasis pathways. It functions in coordination with the transferrin receptor 1 (TfR1) which facilitates cellular uptake of the transferrin-iron complex. Additionally transferrin plays a role in the hepcidin regulatory pathway. Hepcidin modulates iron homeostasis by decreasing iron absorption in the intestine and controlling iron release from macrophages and hepatocytes. Transferrin's ability to bind iron connects it to other iron-containing proteins such as ferritin which stores excess iron in cells.

Several iron-related conditions can impact transferrin function including anemia and hemochromatosis. Anemia often occurs when there is insufficient iron delivery leading to inadequate hemoglobin synthesis and reduced oxygen transport. Aberrant transferrin receptor activity affects iron uptake in such conditions. Hemochromatosis characterized by iron overload can occur due to mutations in genes like HFE leading to changes in hepcidin regulation and increased intestinal iron absorption. Transferrin levels and saturation are clinical indicators used to assess iron status in such diseases.
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Product protocols

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Target data

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
See full target information Tf
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of neurochemistry 165:76-94 PubMed36583241

2023

Ceruloplasmin-deficient mice show changes in PTM profiles of proteins involved in messenger RNA processing and neuronal projections and synaptic processes.

Applications

Unspecified application

Species

Unspecified reactive species

Birgitte Villadsen,Camilla Thygesen,Manuela Grebing,Stefan J Kempf,Marie B Sandberg,Pia Jensen,Stefanie H Kolstrup,Helle H Nielsen,Martin R Larsen,Bente Finsen

Brain sciences 12: PubMed36291201

2022

Identification and Characterization of Elevated Expression of Transferrin and Its Receptor TfR1 in Mouse Models of Depression.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Chang,Mengxin Ma,Liping Chen,Zhihong Song,Zhe Zhao,Wei Shen,Huihui Jiang,Yan Wu,Ming Fan,Haitao Wu

Circulation research 127:651-663 PubMed32450779

2020

Iron-Deficiency and Estrogen Are Associated With Ischemic Stroke by Up-Regulating Transferrin to Induce Hypercoagulability.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaopeng Tang,Mingqian Fang,Ruomei Cheng,Zhiye Zhang,Yuming Wang,Chuanbin Shen,Yajun Han,Qiumin Lu,Yingrong Du,Yingying Liu,Zhaohui Sun,Liping Zhu,James Mwangi,Min Xue,Chengbo Long,Ren Lai

The European respiratory journal 55: PubMed32184317

2020

Crucial role for lung iron level and regulation in the pathogenesis and severity of asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Md Khadem Ali,Richard Y Kim,Alexandra C Brown,Jemma R Mayall,Rafia Karim,James W Pinkerton,Gang Liu,Kristy L Martin,Malcolm R Starkey,Amber L Pillar,Chantal Donovan,Prabuddha S Pathinayake,Olivia R Carroll,Debbie Trinder,Hock L Tay,Yusef E Badi,Nazanin Z Kermani,Yi-Ke Guo,Ritambhara Aryal,Sharon Mumby,Stelios Pavlidis,Ian M Adcock,Jessica Weaver,Dikaia Xenaki,Brian G Oliver,Elizabeth G Holliday,Paul S Foster,Peter A Wark,Daniel M Johnstone,Elizabeth A Milward,Philip M Hansbro,Jay C Horvat

Cell research 30:119-132 PubMed31811276

2019

Transferrin plays a central role in coagulation balance by interacting with clotting factors.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaopeng Tang,Zhiye Zhang,Mingqian Fang,Yajun Han,Gan Wang,Sheng Wang,Min Xue,Yaxiong Li,Li Zhang,Jian Wu,Biqing Yang,James Mwangi,Qiumin Lu,Xiaoping Du,Ren Lai

Hepatology (Baltimore, Md.) 69:2136-2149 PubMed30582749

2019

Low Serum Hepcidin Is Associated With Reduced Short-Term Survival in Adults With Acute Liver Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Igor Spivak,Jyoti Arora,Caitlyn Meinzer,Valerie Durkalski-Mauldin,William M Lee,Christian Trautwein,Robert J Fontana,Pavel Strnad

Nutrition & metabolism 16:13 PubMed30820238

2019

Dietary iron interacts with genetic background to influence glucose homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Mario A Miranda,Celine L St Pierre,Juan F Macias-Velasco,Huyen Anh Nguyen,Heather Schmidt,Lucian T Agnello,Jessica P Wayhart,Heather A Lawson

Nature communications 6:6494 PubMed25751021

2015

MtDNA mutagenesis impairs elimination of mitochondria during erythroid maturation leading to enhanced erythrocyte destruction.

Applications

Unspecified application

Species

Unspecified reactive species

K J Ahlqvist,S Leoncini,A Pecorelli,S B Wortmann,S Ahola,S Forsström,R Guerranti,C De Felice,J Smeitink,L Ciccoli,R H Hämäläinen,A Suomalainen
View all publications
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