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Mouse TREM2 ELISA Kit - Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse TREM2 - Extracellular in Cerebral Spinal Fluid, Cell culture supernatant samples.

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Images

Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (AB309115), expandable thumbnail
  • Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (AB309115), expandable thumbnail
  • Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (AB309115), expandable thumbnail
  • Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (AB309115), expandable thumbnail
  • Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (AB309115), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Sensitivity

= 3.2 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:11241283). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia (PubMed:27477018, PubMed:29518356). Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1 (PubMed:27477018, PubMed:29518356). Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia (PubMed:27477018). Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin (By similarity). Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade (PubMed:28077724). Required for microglial phagocytosis of apoptotic neurons (PubMed:24990881). Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain (PubMed:15728241, PubMed:25631124, PubMed:28592261, PubMed:29752066). Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide (PubMed:28483841, PubMed:29663649, PubMed:29859094, PubMed:30232263). It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB (PubMed:29663649). During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling (PubMed:28592261). Plays a role in microglial MTOR activation and metabolism (PubMed:28802038). Regulates age-related changes in microglial numbers (PubMed:25631124, PubMed:29752066, PubMed:30548312). Triggers activation of the immune responses in macrophages and dendritic cells. Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (PubMed:18957693). In dendritic cells, receptor of SEMA6D with PLEXNA1 as coreceptor and mediates up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed:16715077). Involved in the positive regulation of osteoclast differentiation (PubMed:16418779).

Alternative names

What's included?

1 x 384 Tests
Components
10X Mouse TREM2 (Extracellular) Capture Antibody
1 x 600 µL
10X Mouse TREM2 (Extracellular) Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
384 well CaptSure™ microplates
1 x 1 Unit
Antibody Diluent 4BR
1 x 6 mL
Mouse TREM2 (Extracellular) Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
Stop Solution
2 x 12 mL
TMB Development Solution
2 x 12 mL

Recommended products

Mouse TREM2 ELISA Kit - Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Mouse TREM2 - Extracellular in Cerebral Spinal Fluid, Cell culture supernatant samples.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Mouse

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 3.2 pg/mL

General recovery

99%

Precision

Intra assay

Sample

Supernatant

n

8

mean

-

SD

-

C.V.

1.4

Inter assay

Sample

Supernatant

n

3

mean

-

SD

-

C.V.

2.1

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 99

Range

91 - 106 %

Sample type

Cerebral Spinal Fluid

Average %

= 101

Range

96 - 106 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Mouse TREM2 (Extracellular) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TREM2 (Extracellular) protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Mouse TREM2 (Extracellular) with 3.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TREM2 also known as Triggering Receptor Expressed on Myeloid Cells 2 functions as a receptor with an important role in the immune system. This protein weighing about 230-290 kDa mostly expresses in myeloid cells which include macrophages monocytes and microglia. It serves as an important player in signaling for the activation of these cells. Researchers often study TREM2's functions through various species including cynomolgus monkeys to understand its implications better.

Biological function summary

TREM2 significantly influences immune responses by participating in cellular clearance functions such as phagocytosis. It forms a receptor complex with DAP12 which transduces signals leading to the activation of immune responses. TREM2 aids in regulating inflammatory responses and ensures the maintenance of tissue homeostasis in healthy and diseased states. Its role extends to the control of lipid metabolism particularly in the central nervous system.

Pathways

Scientific studies link TREM2 to the immune-inflammatory pathway and the neurodegenerative pathway. Within these pathways TREM2 interacts notably with proteins such as DAP12 and SYK. Through these interactions TREM2 contributes to signaling cascades that modulate inflammation and neurodegenerative processes within the brain supporting cellular communication and survival.

Associated diseases and disorders

TREM2's functionality connects significantly with Alzheimer's disease and various inflammatory conditions. In Alzheimer's disease mutations in TREM2 alter its normal activity potentially increasing neuroinflammation and advancing disease progression. Additionally collaborations between TREM2 and ApoE proteins further highlight its involvement in lipid regulation within neurodegenerative conditions. Understanding TREM2’s role can pave the way for targeted therapeutic approaches in these disorders.

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5 product images

  • Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (ab309115), expandable thumbnail

    Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (ab309115)

    Example of mouse TREM2 standard curve in 96-well vs 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (ab309115), expandable thumbnail

    Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (ab309115)

    Interpolated concentration of native TREM2 was measured in duplicate at different sample concentrations. Undiluted samples are 20% mouse cerebrospinal fluid. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

  • Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (ab309115), expandable thumbnail

    Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (ab309115)

    Interpolated concentration of native mouse TREM2 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1% J774A.1 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

  • Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (ab309115), expandable thumbnail

    Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (ab309115)

    Example of mouse TREM2 standard curve. Background-subtracted data values (mean +/- SD) are graphed. 

  • Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (ab309115), expandable thumbnail

    Sandwich ELISA - Mouse TREM2 ELISA Kit - Extracellular (ab309115)

    Interpolated concentration of native TREM2 was measured in duplicate at different sample concentrations. Undiluted samples are 1% J774A.1 cell culture supernatant.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. 

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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