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AB285235

Mouse Troponin I ELISA Kit

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(3 Publications)

Mouse Troponin I ELISA Kit is a sandwich ELISA designed to quantify Mouse Troponin I with a sensitivity of 18.75 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 31.25 - 2000 pg/mL

View Alternative Names

Tnni1

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Sandwich ELISA - Mouse Troponin I ELISA Kit (AB285235)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Troponin I ELISA Kit (AB285235)

Mouse Troponin I ELISA Kit.

Typical standard curve and data is provided below for reference only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Other biological fluids

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 18.75 pg/mL

Range

31.25 - 2000 pg/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

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Product details

Mouse Troponin I ELISA Kit ab285235 is a sandwich ELISA to measure Mouse Troponin I in serum, plasma, other biological fluids with a sensitivity of 18.75 pg/ml.

How the assay works

The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to mouse Troponin I. Standards or samples were added to the micro ELISA plate that reacts with biotinylated detection antibody specific for mouse Troponin I and Avidin-Horseradish Peroxidase (HRP) conjugate which on the addition of substrate form blue color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of human Troponin I. The concentration of mouse Troponin I in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse Troponin I ELISA Kit ab285235 protocol summary

1. Add standards and samples to appropriate wells.Incubate
2. Wash
3. Add Biotinylated Detection Antibody. Incubate
4. Wash
5. Add HRP Conjugate. Incubate
6. Wash
7. Add Substrate Reagent. Incubate
8. Add stop solution and read at 450 nm

This product is manufactured by BioVision, an Abcam company and was previously called E4736 Troponin I (Mouse) ELISA Kit. E4736-100 is the same size as the 96 test size of ab285235.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
See full target information Tnni1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed38456501

2024

Macrophage-enriched Sectm1a promotes efficient efferocytosis to attenuate ischemia/reperfusion-induced cardiac injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Wang,Wa Du,Yutian Li,Hui-Hui Yang,Yu Zhang,Rubab Akbar,Hannah Morgan,Tianqing Peng,Jing Chen,Sakthivel Sadayappan,Yueh-Chiang Hu,Yanbo Fan,Wei Huang,Guo-Chang Fan

Nature communications 14:3603 PubMed37330510

2023

A pharmacoproteomic landscape of organotypic intervention responses in Gram-negative sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Tirthankar Mohanty,Christofer A Q Karlsson,Yashuan Chao,Erik Malmström,Eleni Bratanis,Andrietta Grentzmann,Martina Mørch,Victor Nizet,Lars Malmström,Adam Linder,Oonagh Shannon,Johan Malmström

The Journal of pathology 260:276-288 PubMed37185821

2023

IFN-γ and androgens disrupt mitochondrial function in murine myocytes.

Applications

Unspecified application

Species

Unspecified reactive species

John M Fenimore,Danielle A Springer,Maria E Romero,Elijah F Edmondson,Dan W McVicar,Sudhirkumar Yanpallewar,Michael Sanford,Thea Spindel,Elizabeth Engle,Thomas J Meyer,Julio C Valencia,Howard A Young
View all publications
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websiteProtocolBooklet
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