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AB155461

Mouse TSLP ELISA Kit

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(5 Publications)

Mouse TSLP ELISA Kit is a Sandwich ELISA for the measurement of Mouse TSLP in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Thymic stromal lymphopoietin, Thymic stroma-derived lymphopoietin, Tslp

2 Images
Sandwich ELISA - Mouse TSLP ELISA Kit (AB155461)
  • sELISA

Unknown

Sandwich ELISA - Mouse TSLP ELISA Kit (AB155461)

Representative standard curve using ab155461 - Assay Diluent B.

Sandwich ELISA - Mouse TSLP ELISA Kit (AB155461)
  • sELISA

Unknown

Sandwich ELISA - Mouse TSLP ELISA Kit (AB155461)

Representative standard curve using ab155461 - Assay Diluent A.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 1 pg/mL

Range

0.82 - 200 pg/mL

Assay Platform

Microplate

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Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's TSLP Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse TSLP in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for mouse TSLP coated on a 96- well plate. Standards and samples are pipetted into the wells and TSLP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TSLP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TSLP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 125 %", "average": "= 126.4" }, { "sample": "Serum", "range": "105 - 129 %", "average": "= 117.5" }, { "sample": "Plasma", "range": "86 - 122 %", "average": "= 98.55" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155461-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB155461-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155461-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155461-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155461-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse TSLP", "number":"AB155461-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"TSLP Microplate (12 x 8 wells)", "number":"AB155461-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse TSLP Standard (lyophilized)", "number":"AB155461-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155461-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB155461-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSLP or thymic stromal lymphopoietin is a cytokine protein weighing around 27 kDa. It is also known as a hematopoietic cytokine and functions in the signaling processes of the immune system. TSLP is expressed in epithelial cells most notably in the skin lungs and gut. This cytokine holds significance due to its role in immune responses acting through its receptor complex made of TSLP receptor (TSLPR) and the interleukin-7 receptor alpha chain (IL-7Rα).
Biological function summary

TSLP plays a role in mediating immune system responses. It induces the differentiation of T-helper 2 (Th2) cells promoting a specific type of immune response. TSLP does not appear to be part of a larger protein complex but it often functions in association with the TSLPR and IL-7Rα receptor complex. It influences the activity and maturation of dendritic cells which are important for initiating immune responses.

Pathways

A mechanical understanding of TSLP reveals its involvement in both the JAK-STAT and NF-kB signaling pathways. It interacts with proteins like JAK2 and STAT5 which are important for the downstream signaling cascade leading to inflammation and immune responses. These pathways play important roles in the immune system's ability to detect and respond to environmental triggers.

TSLP has relevance in conditions like asthma and atopic dermatitis. It promotes inflammation that contributes to these allergic diseases. The dysregulation of TSLP expression can exacerbate these conditions. In asthma TSLP interacts with proteins such as interleukin-33 (IL-33) to enhance inflammatory responses in airway tissues. In atopic dermatitis connections with interleukin-4 (IL-4) are noted as both cytokines contribute to the inflammatory processes involved in the disease pathology. These associations make TSLP a potential target for therapeutic interventions in such disorders.
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Product protocols

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Target data

Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells (By similarity).
See full target information Tslp
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Clinical, cosmetic and investigational dermatology 17:1093-1105 PubMed38765196

2024

Effect of Chloroquine on Type 2 Inflammatory Response in MC903-Induced Atopic Dermatitis Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjing Wei,Huixue Yang,Zhengchao Shao,Haoyue Wan,Yiheng Wang,Wenqi Chen

Annals of translational medicine 10:980 PubMed36267759

2022

Inhibitory effects of isoliquiritin on an atopic dermatitis model through the CD177/JAK2/STAT pathway and .

Applications

Unspecified application

Species

Unspecified reactive species

Qing Wu,Xiumei Mo,Ying Lin,Junfeng Liu,Siqi Ye,Yu Zhang,Xingxing Fan,Dacan Chen,Fenggen Yan

Annals of translational medicine 10:209 PubMed35280398

2022

Polyinosinic:polycytidylic acid aggravates calcipotriol-induced atopic dermatitis-like skin lesions in mice by increasing the expression of thymic stromal lymphopoietin.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyue Wan,Huixue Yang,Mingjing Wei,Wenqi Chen

Scientific reports 10:8417 PubMed32439906

2020

Caffeoyl-Pro-His amide relieve DNCB-Induced Atopic Dermatitis-Like phenotypes in BALB/c mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sunhyae Jang,Jungyoon Ohn,Ji Won Kim,So Min Kang,Dasom Jeon,Chan Yeong Heo,Yoon-Sik Lee,Ohsang Kwon,Kyu Han Kim

The Journal of investigative dermatology 140:1524-1532 PubMed32004565

2020

PAR2 Mediates Itch via TRPV3 Signaling in Keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Zhao,Admire Munanairi,Xian-Yu Liu,Jie Zhang,Linghan Hu,Meiqin Hu,Dingfang Bu,Lingling Liu,Zhiqiang Xie,Brian S Kim,Yong Yang,Zhou-Feng Chen
View all publications
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websiteProtocolBooklet
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Product promise

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