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AB316259

Mouse TSLP ELISA Kit

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Mouse TSLP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse TSLP with a sensitivity of 5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Thymic stromal lymphopoietin, Thymic stroma-derived lymphopoietin, Tslp

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Sandwich ELISA - Mouse TSLP ELISA Kit (AB316259)
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Supplier Data

Sandwich ELISA - Mouse TSLP ELISA Kit (AB316259)

Serum from nine individual healthy female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Prostaglandin E2 concentration was determined to be 287 pg/mL with a range of 92 – 585 pg/mL.

Sandwich ELISA - Mouse TSLP ELISA Kit (AB316259)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TSLP ELISA Kit (AB316259)

Standard curve comparison between Mouse TSLP SimpleStep ELISA kit (ab316259) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows comparable sensitivity.

Sandwich ELISA - Mouse TSLP ELISA Kit (AB316259)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse TSLP ELISA Kit (AB316259)

Interpolated concentration of native TSLP was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 12.5%, plasma (EDTA) 25%, plasma (heparin) 50% and lung tissue supernatant 100%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Cell culture supernatant, Citrate plasma, Heparin Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 5 pg/mL

Range

14.063 - 900 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse TSLP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TSLP protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Mouse TSLP with 5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" } ]
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Recovery

[ { "sample": "Serum", "range": "115 - 117 %", "average": "= 116" }, { "sample": "Citrate plasma", "range": "115 - 128 %", "average": "= 121" }, { "sample": "EDTA Plasma", "range": "81 - 123 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "104 - 131 %", "average": "= 114" }, { "sample": "Cell culture supernatant", "range": "101 - 126 %", "average": "= 110" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab316259-96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB316259-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB316259-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse TSLP Lyophilized Recombinant Protein", "number":"AB316259-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse TSLP Capture Antibody", "number":"AB316259-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316259-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB316259-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB316259-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB316259-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB316259-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse TSLP Detector Antibody", "number":"AB316259-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSLP or thymic stromal lymphopoietin is a cytokine protein weighing around 27 kDa. It is also known as a hematopoietic cytokine and functions in the signaling processes of the immune system. TSLP is expressed in epithelial cells most notably in the skin lungs and gut. This cytokine holds significance due to its role in immune responses acting through its receptor complex made of TSLP receptor (TSLPR) and the interleukin-7 receptor alpha chain (IL-7Rα).
Biological function summary

TSLP plays a role in mediating immune system responses. It induces the differentiation of T-helper 2 (Th2) cells promoting a specific type of immune response. TSLP does not appear to be part of a larger protein complex but it often functions in association with the TSLPR and IL-7Rα receptor complex. It influences the activity and maturation of dendritic cells which are important for initiating immune responses.

Pathways

A mechanical understanding of TSLP reveals its involvement in both the JAK-STAT and NF-kB signaling pathways. It interacts with proteins like JAK2 and STAT5 which are important for the downstream signaling cascade leading to inflammation and immune responses. These pathways play important roles in the immune system's ability to detect and respond to environmental triggers.

TSLP has relevance in conditions like asthma and atopic dermatitis. It promotes inflammation that contributes to these allergic diseases. The dysregulation of TSLP expression can exacerbate these conditions. In asthma TSLP interacts with proteins such as interleukin-33 (IL-33) to enhance inflammatory responses in airway tissues. In atopic dermatitis connections with interleukin-4 (IL-4) are noted as both cytokines contribute to the inflammatory processes involved in the disease pathology. These associations make TSLP a potential target for therapeutic interventions in such disorders.
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Product protocols

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Target data

Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells (By similarity).
See full target information Tslp
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websiteProtocolBooklet
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