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AB113320

Mouse Tweak ELISA Kit

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Mouse Tweak ELISA Kit is a Sandwich ELISA for the measurement of Mouse Tweak in Mouse in Biofluids, Cell Culture Media samples.

View Alternative Names

Tumor necrosis factor ligand superfamily member 12, TNF-related weak inducer of apoptosis, TWEAK, Tnfsf12

1 Images
Sandwich ELISA - Mouse Tweak ELISA Kit (AB113320)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse Tweak ELISA Kit (AB113320)

Representative Standard Curve using ab113320.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Mouse

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 0.3 pg/mL

Range

0.27 - 200 pg/mL

Assay Platform

Microplate

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Primary Antibodies

AB37170

Anti-TWEAK antibody

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Tweak Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Tweak in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Tweak coated on a 96-well plate. Standards and samples are pipetted into the wells and Tweak present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Tweak antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Tweak bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "68 - 83 %", "average": "= 75.69" }, { "sample": "Serum", "range": "71 - 87 %", "average": "= 79.66" }, { "sample": "Plasma", "range": "70 - 89 %", "average": "= 77.72" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab113320-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB113320-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB113320-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Tweak Microplate (12 strips x 8 wells)", "number":"AB113320-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB113320-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB113320-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Mouse Tweak", "number":"AB113320-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB113320-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse Tweak Standard (lyophilized)", "number":"AB113320-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB113320-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TWEAK also known as Tumor Necrosis Factor-like Weak Inducer of Apoptosis is a type II transmembrane cytokine with a mass of approximately 24 kDa. This protein identified by the human gene TNFSF12 is widely expressed in several tissues including the heart pancreas and skeletal muscle. TWEAK also exists in a soluble form which can arise through proteolytic cleavage. The protein plays various roles in cellular functions by interacting with its receptor Fn14 initiating signaling cascades that affect cell fate.
Biological function summary

TWEAK influences processes such as cell proliferation differentiation and apoptosis. It does not form part of a larger complex but functions independently to mediate cellular activities. The interaction with Fn14 its sole receptor leads to the activation of several downstream signaling pathways. TWEAK's involvement is prominent in angiogenesis inflammatory responses and tissue remodeling suggesting its significance in these biological processes.

Pathways

TWEAK has significant influence on the Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinases (MAPK) pathways. The interaction of TWEAK with NF-kB can enhance the expression of pro-inflammatory cytokines while its effect on MAPK regulates cell survival and death. TWEAK closely relates to proteins such as TNF-alpha in these pathways often working together to modulate immune responses and cellular environments.

TWEAK is associated with conditions such as rheumatoid arthritis and multiple sclerosis. Its elevated levels have been observed in the synovial tissues of patients with rheumatoid arthritis suggesting its potential role in promoting inflammation. Similarly in multiple sclerosis TWEAK contributes to neuroinflammatory activities. TNF-alpha also links to TWEAK through these diseases showing that both proteins often interact in inflammatory situations which may suggest possible targets for therapeutic interventions.
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Product protocols

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Target data

Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion (By similarity).
See full target information Tnfsf12
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websiteProtocolBooklet
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Product promise

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