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AB245727

Mouse uPA ELISA Kit

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Mouse uPA ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse uPA with a sensitivity of 31.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Urokinase-type plasminogen activator, U-plasminogen activator, uPA, Plau

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ELISA - Mouse uPA ELISA Kit (AB245727)
  • ELISA

Supplier Data

ELISA - Mouse uPA ELISA Kit (AB245727)
Sandwich ELISA - Mouse uPA ELISA Kit (AB245727)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse uPA ELISA Kit (AB245727)

Interpolated concentrations of native uPA in mouse serum, plasma samples. The concentrations of uPA were measured in duplicates, interpolated from the uPA standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 25%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean uPA concentration was determined to be 7166 pg/mL in serum, 6222 pg/mL in plasma (citrate) and 7060 pg/mL in plasma (EDTA), and 4907 pg/mL in plasma (heparin).

Sandwich ELISA - Mouse uPA ELISA Kit (AB245727)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse uPA ELISA Kit (AB245727)

Interpolated concentrations of native uPA in mouse urine. The concentrations of uPA were measured in duplicates, interpolated from the uPA standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean uPA concentration was determined to be 272.6 ng/mL in urine.

Sandwich ELISA - Mouse uPA ELISA Kit (AB245727)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse uPA ELISA Kit (AB245727)

Interpolated concentrations of spiked uPA in cell culture media. The concentrations of uPA were measured in duplicates, interpolated from the uPA standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse uPA ELISA Kit (AB245727)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse uPA ELISA Kit (AB245727)

Example of mouse uPA standard curve in Sample Diluent NS. The uPA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

= 31.7 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Mouse uPA ELISA Kit ab245727 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse uPA in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 31.7 pg/mL.

How the assay works

Mouse uPA SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse uPA ELISA Kit ab245727 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-uPA antibody [EPR22151-5] - BSA and Azide free (Capture) ab259584
- Anti-uPA antibody [EPR22151-66] - BSA and Azide free (Detector) ab259584
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]
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Recovery

[ { "sample": "Serum", "range": "103 - 118 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "108 - 120 %", "average": "= 113" }, { "sample": "Urine", "range": "101 - 124 %", "average": "= 112" }, { "sample": "Cell culture media", "range": "91 - 99 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "105 - 114 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "104 - 106 %", "average": "= 106" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab245727-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB245727-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB245727-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB245727-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB245727-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB245727-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse uPA Capture Antibody", "number":"AB245727-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse uPA Lyophilized Recombinant Protein", "number":"AB245727-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB245727-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB245727-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse uPA Detector Antibody", "number":"AB245727-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
See full target information Urokinase-type plasminogen activator
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websiteProtocolBooklet
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