Mouse uPA Receptor/U-PAR ELISA Kit

Product details

The Mouse uPA Receptor/U-PAR Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213896) is designed for the quantitative detection of Mouse uPAR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for uPAR has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: L24-T297) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for uPA Receptor/U-PAR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse uPA Receptor/U-PAR amount of sample captured in plate.

The urokinase-type plasminogen activator receptor (uPA Receptor/U-PAR) is a key molecule in the regulation of cell surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes. The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosylphosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator. uPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer. PLAUR is located at chromosome 19q13.1-q13.2.1.